1887

Abstract

Human immunodeficiency virus type 1 (HIV-1) infection of the CD4 SupT and CEM cell lines, blocked in cell replication by the polymerase α inhibitor aphidicolin (APC), was studied. The APC-treated cells showed a lack of viral production, but the presence of single cell killing. High levels of unintegrated viral DNA forms were found in the infected APC-treated cells as compared with untreated cells. Moreover, an increased rate of viral replication occurred in the remaining viable cells following removal of APC. The results indicate that HIV-1 entry and reverse transcription can take place in cells blocked in the S phase of the cell cycle. Replication of infectious progeny virions appears to require cell division. Finally, accumulation of viral DNA in cells during APC treatment can result in cytopathological effects and subsequent enhancement of virus production.

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1992-04-01
2022-09-25
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References

  1. Besanky N. J., Butera S. T., Sinha S., Folks T. M. 1991; Unintegrated human immunodeficiency virus type 1 DNA in chronically infected cell lines is not correlated with surface CD4 expression. Journal of Virology 65:2695–2698
    [Google Scholar]
  2. Brahic M., Haase A. T. 1978; Detection of viral sequences of low reiteration frequency by in situ hybridization. Proceedings of the National Academy of Sciences, U.S.A 75:6125–6129
    [Google Scholar]
  3. Chinsky J., Soeiro R. 1981; Fv-1 host restriction of Friend leukemia virus: analysis of unintegrated proviral DNA. Journal of Virology 40:45–55
    [Google Scholar]
  4. Gowda S. D., Stein B. S., Mohagheghpour N., Benike C. J., Englemann E. G. 1989; Evidence that T cell activation is required for HIV-1 entry in CD4+ lymphocytes. Journal of Immunology 142:773–780
    [Google Scholar]
  5. Haase A. T., Stworing L., Narayan O., Griffin D., Price D. 1977; Slow persistent infection caused by visna virus: role of host restriction. Science 195:175–177
    [Google Scholar]
  6. Hoffman A. D., Banapour B., Levy J. A. 1985; Characterization of the AIDS-associated retrovirus reverse transcriptase and optimal conditions for its detection in virions. Virology 147:326–335
    [Google Scholar]
  7. Hsu T. W., Taylor J. M. 1982; Effect of aphidicolin on avian sarcoma virus replication. Journal of Virology 44:493–498
    [Google Scholar]
  8. Huberman J. A. 1981; New view of biochemistry of eucaryotic DNA replication revealed by aphidicolin, an unusual inhibitor of DNA polymerase alpha. Cell 23:647–648
    [Google Scholar]
  9. Humphries E. H., Glove C., Reichmann M. E. 1981; Rous sarcoma virus infection of synchronized cells establishes provirus integration during S-phase DNA synthesis prior to cellular division. Proceedings of the National Academy of Sciences, U.S.A 78:2601–2605
    [Google Scholar]
  10. Kaminsky L. S., McHugh L. T., Stites D., Volberding P., Henle G., Henle W., Levy J. A. 1985; High prevalence of antibodies to acquired immune deficiency syndrome (AIDS)-associated retrovirus (ARV) in AIDS and related conditions but not in other disease states. Proceedings of the National Academy of Sciences, U.S.A 82:5535–5539
    [Google Scholar]
  11. Keshet E., Temin H. M. 1979; Cell killing by spleen necrosis virus is correlated with a transient accumulation of spleen necrosis virus DNA. Journal of Virology 31:376–388
    [Google Scholar]
  12. Krishan A. 1975; Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining. Journal of Cellular Biology 66:188–193
    [Google Scholar]
  13. Levy J. A., Shimabukuro J. 1985; Recovery of AIDS-associated retroviruses from patients with AIDS or AIDS-related conditions and from clinically healthy individuals. Journal of Infectious Diseases 152:734–738
    [Google Scholar]
  14. Levy J. A., Hoffman A. D., Kramer S. M., Landis J. A., Shimabukuro J. M., Oshiro L. S. 1984; Isolation of lymphocytopathic retroviruses from San Francisco patients with AIDS. Science 225:840–842
    [Google Scholar]
  15. Levy J. A., Kaminsky L. S., Morrow W. J. W., Steimer K., Luciw P., Dina D., Hoxie J., Oshiro L. 1985; Infection by the retrovirus associated with the acquired immunodeficiency syndrome. Annals of Internal Medicine 103:694–699
    [Google Scholar]
  16. Luciw P. A., Potter S. J., Steimer K., Dina D., Levy J. A. 1984; Molecular cloning of AIDS-associated retrovirus. Nature, London 312:760–763
    [Google Scholar]
  17. Masuda T., Harada S. 1990; Unintegrated DNA in cells infected in vitro with human immunodeficiency virus (HIV): a new approach to suppression of virus release. Journal of Virological Methods 28:219–234
    [Google Scholar]
  18. Mullins J. I., Chen C. S., Hoover E. A. 1986; Disease-specific and tissue-specific production of unintegrated feline leukaemia virus variant DNA in feline AIDS. Nature, London 319:333–336
    [Google Scholar]
  19. Nilsen T., Baglioni C. 1979; Unusual base-pairing of newly synthesized DNA in HeLa cells. Journal of Molecular Biology 133:319–338
    [Google Scholar]
  20. Pang S., Koyanagi Y., Miles S., Wiley C., Vinters H. V., Chen I. S. Y. 1990; High levels of unintegrated HIV-1 DNA in brain tissue of AIDS dementia patients. Nature, London 343:85–89
    [Google Scholar]
  21. Pauza C. D., Galindo J. 1989; Persistent human immunodeficiency virus type 1 infection of monoblastoid cells leads to accumulation of self-integrated viral DNA and to production of defective virions. Journal of Virology 63:3700–3707
    [Google Scholar]
  22. Pedrali-Noy G., Spadari S., Miller-Fanres A., Miller A. O. A., Kruppa J., Koch G. 1980; Synchronization of HeLa cell cultures by inhibition of DNA polymerase with aphidicolin. Nucleic Acids Research 8:377–387
    [Google Scholar]
  23. Pellicer A., Wigler M., Axel R. 1984; The transfer and stable integration of the HSV thymidine kinase gene into mouse cells. Cell 14:133–141
    [Google Scholar]
  24. Price R. W., Brew B., Sidtis J., Rosenblum M., Scheck A. C., Cleary P. 1988; The brain in AIDS: central nervous system HIV-1 infection and AIDS dementia complex. Science 239:586–592
    [Google Scholar]
  25. Richten A., Ozer H., Desgroseillers L., Jolicoeur P. 1984; An X-linked gene affecting mouse cell DNA synthesis also affects production of unintegrated linear and supercoiled DNA of murine leukemia virus. Molecular and Cellular Biology 4:151–159
    [Google Scholar]
  26. Robinson H. L., Zinkus D. M. 1990; Accumulation of human immunodeficiency virus type 1 DNA in T cells: result of multiple infection events. Journal of Virology 64:4836–4841
    [Google Scholar]
  27. Shaw G. M., Hahn B. H., Arya S. K., Groopman J. E., Gallo R. C., Wong-Staal F. 1984; Molecular characterization of human T-cell leukemia (lymphotropic) virus type III in the acquired immune deficiency syndrome. Science 226:1165–1171
    [Google Scholar]
  28. Shaw G. M., Harper M. E., Hahn B. H. 1985; HTLV-III infection in brains of children and adults with AIDS encephalopathy. Science 227:177–181
    [Google Scholar]
  29. Springett G. M., Moen R. C., Anderson S., Blaese R., Anderson W. 1989; Infection efficiency of T lymphocytes with amphotropic retroviral vectors is cell cycle dependent. Journal of Virology 63:3865–3869
    [Google Scholar]
  30. Tang S. B., Levy J. A. 1990; Parameters involved in the cell fusion induced by the human immunodeficiency viruses. AIDS 4:409–415
    [Google Scholar]
  31. Tang S. B., Levy J. A. 1991; Inactivation of HIV-1 by trypsin and its use in demonstrating specific virus infection of cells. Journal of Virological Methods 33:39–46
    [Google Scholar]
  32. Tateno M., Levy J. A. 1988; MT-4 plaque formation can distinguish cytopathic subtypes of the human immunodeficiency virus (HIV). Virology 167:299–301
    [Google Scholar]
  33. Varmus H., Padgett T., Heasley S., Simon G., Bishop J. 1977; Cellular functions are required for the synthesis and integration of avian sarcoma virus specific DNA. Cell 11:307–319
    [Google Scholar]
  34. Watkins B. A., Dorn H. H., Kelly W. B., Armstrong R. C., Dubois-Dalcq M. 1990; Specific tropism of HIV-1 for microglial cells in primary human brain culture. Science 249:549–553
    [Google Scholar]
  35. Wiley C. A., Schrier R. D., Nelson J. A., Lampert P. W., Oldstone M. B. A. 1986; Cellular localization of human immunodeficiency virus infection within the brains of acquired immune deficiency syndrome patients. Proceedings of the National Academy of Sciences, U.S.A 83:7089–7093
    [Google Scholar]
  36. Yang W. K., Yang D. M., Kiggans J. O. 1980; Covalently closed circular DNAs of murine type C retrovirus: depressed formation in cells treated with cycloheximide early after infection. Journal of Virology 36:181–188
    [Google Scholar]
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