Bovine coronavirus uses -acetyl-9--acetylneuraminic acid as a receptor determinant to initiate the infection of cultured cells Free

Abstract

The importance of -acetyl-9--acetylneuraminic acid (Neu5,9Ac) as a receptor determinant for bovine coronavirus (BCV) on cultured cells was analysed. Pretreatment of MDCK I (Madin Darby canine kidney) cells with neuraminidase or acetylesterase rendered the cells resistant to infection by BCV. The receptors on a human (CaCo-2) and a porcine (LLC-PK1) epithelial cell line were also found to be sensitive to neuraminidase treatment. The susceptibility to infection by BCV was restored after resialylation of asialo-MDCK I cells with Neu5,9Ac. Transfer of sialic acid lacking a 9--acetyl group was ineffective in this respect. These results demonstrate that 9--acetylated sialic acid is used as a receptor determinant by BCV to infect cultured cells. The possibility is discussed that the initiation of a BCV infection involves the recognition of different types of receptors, a first receptor for primary attachment and a second receptor to mediate the fusion between the viral envelope and the cellular membrane.

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1992-04-01
2024-03-29
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References

  1. Boyle J. F., Weismiller D. G., Holmes K. V. 1987; Genetic resistance to mouse hepatitis virus correlates with absence of virus-binding activity on target tissues. Journal of Virology 61:185–189
    [Google Scholar]
  2. Carroll S. M., Paulson J. C. 1985; Differential infection of receptor-modified host cells by receptor-specific influenza viruses. Virus Research 3:165–179
    [Google Scholar]
  3. Herrler G., Klenk H.-D. 1987; The surface receptor is a major determinant of the cell tropism of influenza C virus. Virology 159:102–108
    [Google Scholar]
  4. Herrler G., Klenk H.-D. 1991; Structure and function of the HEF glycoprotein of influenza C virus. Advances in Virus Research 40:213–234
    [Google Scholar]
  5. Herrler G., Rott R., Klenk H.-D. 1985a; Neuraminic acid is involved in the binding of influenza C virus to erythrocytes. Virology 141:144–147
    [Google Scholar]
  6. Herrler G., Rott R., Klenk H.-D., Műller H.-P., Shukla A. K., Schauer R. 1985b; The receptor-destroying enzyme of influenza C virus is neuraminate-O-acetylesterase . EMBO Journal 4:1503–1506
    [Google Scholar]
  7. King B., Potts B. J., Brian D. A. 1985; Bovine coronavirus hemagglutinin protein. Virus Research 2:1010–1013
    [Google Scholar]
  8. Markwell M. A. K., Svennerholm L., Paulson J. C. 1981; Specific gangliosides function as host cell receptors for Sendai virus. Proceedings of the National Academy of Sciences, U,. S,. A 78:5406–5410
    [Google Scholar]
  9. Payne H. R., Storz J. 1988; Analysis of cell fusion induced by bovine coronavirus infection. Archives of Virology 103:27–33
    [Google Scholar]
  10. Rogers G. N., Herrler G., Paulson J. C., Klenk H.-D. 1986; Influenza C virus uses 9-O-acetyl-N-acetylneuraminic acid as a high affinity receptor determinant for attachment to cells. Journal of Biological Chemistry 261:5947–5951
    [Google Scholar]
  11. Sato K., Inaba Y., Kurogi H., Takahashi E., Satoda K., Omori T., Matumoto M. 1977; Hemagglutination by calf diarrhea coronavirus. Veterinary Microbiology 2:83–87
    [Google Scholar]
  12. Schultze B., Gross H.-J., Brossmer R., Klenk H.-D., Herrler G. 1990; Hemagglutinating encephalomyelitis virus attaches to N- acetyl-9-O-acetylneuraminic acid-containing receptors on erythrocytes: comparison with bovine coronavirus and influenza C virus. Virus Research 16:185–194
    [Google Scholar]
  13. Schultze B., Wahn K., Klenk H.-D., Herrler G. 1991a; Isolated HE protein from hemagglutinating encephalomyelitis virus and bovine coronavirus has receptor-destroying and receptor-binding activity. Virology 180:221–228
    [Google Scholar]
  14. Schultze B., Gross H.-J., Brossmer R., Herrler G. 1991b; The S protein of bovine coronavirus is a hemagglutinin recognizing 9-O-acetylated sialic acid as a receptor determinant. Journal of Virology 65:6232–6237
    [Google Scholar]
  15. Siddell S., Wege H., ter Meulen V. 1983; The biology of coronaviruses. Journal of General Virology 64:761–776
    [Google Scholar]
  16. Skehel J. J., Bayley P. M., Brown E. B., Martin S. R., Waterfield M. D., White J. M., Wi I. A., Wiley D. C. 1982; Changes in the conformation of influenza virus hemagglutinin at the pH optimum of virus-mediated membrane fusion. Proceedings of the National Academy of Sciences, U,. S,. A 79:968–972
    [Google Scholar]
  17. Sugiyama K., Amano Y. 1980; Hemagglutination and structural polypeptides of a new coronavirus associated with diarrhea in infant mice. Archives of Virology 66:95–105
    [Google Scholar]
  18. Taguchi F., Massa P. T., ter Meulen V. 1986; Characterization of a variant isolated from neural cell culture after infection of mouse coronavirus HJMV. Virology 155:267–270
    [Google Scholar]
  19. Vlasak R., Luytjes W., Spaan W., Palese P. 1988a; Human and bovine coronaviruses recognize sialic acid containing receptors similar to those of influenza C virus. Proceedings of the National Academy of Sciences, U,. S,. A 85:4526–4529
    [Google Scholar]
  20. Vlasak R., Luytjes W., Leider J., Spaan W., Palese P. 1988b; The E3 protein of bovine coronavirus is a receptor-destroying enzyme with acetylesterase activity. Journal of Virology 62:4686–4690
    [Google Scholar]
  21. Williams R. K., Jiang G.-S., Snyder S. W., Frana M. F., Holmes K. V. 1990; Purification of the 110-kilodalton glycoprotein receptor for mouse hepatitis virus (MHV)-A59 from mouse liver and identification of a nonfunctional, homologous protein in MHV-resistant SJL/J mice. Journal of Virology 64:3817–3823
    [Google Scholar]
  22. Williams R. K., Jiang G.-S., Holmes K. V. 1991; Receptor for mouse hepatitis virus is a member of the carcinoembryonic antigen family of glycoproteins. Proceedings of the National Academy of Sciences, U,. S,. A 88:5533–5536
    [Google Scholar]
  23. Yokomori L. La, Monica N., Makino S., Shieh C.-K., Lai M. M. C. 1989; Biosynthesis, structure, and biological activities of envelope protein gp65 of murine coronavirus. Virology 137:683–691
    [Google Scholar]
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