1887

Abstract

The prototype Puumala virus (PV) Sotkamo strain small (S) and medium (M) RNA genome segments were amplified by the polymerase chain reaction (PCR), cloned and sequenced. The S segment is 1830 nucleotides long with an open reading frame coding for 433 amino acids. The identity to the PV Hällnäs strain was 83% at the nucleotide and 96% at the amino acid level. The M segment in the Sotkamo strain is 3616 nucleotides long and contains one open reading frame of 1148 amino acids with 83% nucleotide and 94% amino acid identity to the Hällnäs strain. Most amino acid changes were conservative and the five predicted glycosylation sites are identical. The amino acid identity to the prototype hantavirus, Hantaan virus, was 62 and 54% for S and M segments, respectively. The coding region of the S segment was further amplified by PCR, ligated to pEX vectors and expressed in as a β-galactosidase fusion protein and was seen to be specifically detected by nephropathia epidemica sera in immunoblotting.

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1992-04-01
2022-09-30
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References

  1. Antic D., Byung-Uk L., Kang Y. 1991a; Molecular characterization of the M genomic segment of the Seoul 80-39 virus; nucleotide and amino acid sequence comparisons with other hanta viruses reveal the evolutionary pathway. Virus Research 19:47–58
    [Google Scholar]
  2. Antic D., Byung-Uk L., Kang Y. 1991b; Nucleotide sequence and coding capacity of the large (L) genomic RNA segment of Seoul 80-39 virus, a member of the hanta virus genus. Virus Research 19:59–66
    [Google Scholar]
  3. Arikawa J., Schmaljohn A. L., Dalrymple J. M., Schmaljohn C. S. 1989; Characterization of Hantaan virus envelope glycoprotein antigenic determinants defined by monoclonal antibodies. Journal of General Virology 70:615–624
    [Google Scholar]
  4. Arikawa J., Lapenotiere H. F., Iacono-Connors L., Wang M., Schmaljohn C. S. 1990; Coding properties of the S and M segments of Sapporo rat: comparison to other causative agents of hemorrhagic fever with renal syndrome. Virology 176:114–125
    [Google Scholar]
  5. Battles J. K., Dalrymple J. M. 1988; Genetic variation among geographic isolates of Rift Valley fever virus. American Journal of Tropical Medicine and Hygiene 39:617–631
    [Google Scholar]
  6. Beaty B. J., Calisher C. H. 1991; Bunyaviridae-natural history. In Current Topics in Microbiology and Immunology, Bunyaviridae vol 169 pp 000–00 Edited by Kolakofsky D. New York, Berlin: Heidelberg:Springer-Verlag;
    [Google Scholar]
  7. Brummer-Korvenkontio M., Vaheri A., Hovi T., von Bonsdorff C.-H., Vuorimies J., Manni T., Penttinen K., Oker-Blom N., Lahdevirta J. 1980; Nephropathia epidemica: detection of antigen in bank voles and serologic diagnosis of human infection. Journal of Infectious Diseases 141:131–134
    [Google Scholar]
  8. Brummer-Korvenkontio M., Henttonen H., Vaheri A. 1982; Hemorrhagic fever with renal syndrome in Finland: ecology and virology of nephropathia epidemica. Scandinavian Journal of Infectious Diseases supplement 3688–91
    [Google Scholar]
  9. Calisher C. H. 1990; Editor’s comments. Archives of Virology supplement 11–3
    [Google Scholar]
  10. Chung C. T., Niemalä S. T., Miller R. H. 1989; One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proceedings of the National Academy of Sciences, U,. S,. A 86:2172–2175
    [Google Scholar]
  11. Cohen S. N., Chang A. C. Y., Hsu L. 1972; Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escheri-chia coli by R-factor DNA. Proceedings of the National Academy of Sciences, U,. S,. A 69:2110–2115
    [Google Scholar]
  12. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  13. Elliott R. M. 1990; Molecular biology of the Bunyaviridae. Journal of General Virology 71:501–522
    [Google Scholar]
  14. Giebel L. B., Stohwasser R., Zöller L., Bautz E. K. F., Darai G. 1989; Determination of the coding capacity of the M genome segment of nephropathia epidemica virus strain Hällnäs B1 by molecular cloning and nucleotide sequence analysis. Virology 172:498–505
    [Google Scholar]
  15. Giebel L. B., Zöller L., Bautz E. K. F., Darai G. 1990; Rapid detection of genomic variations in different strains of hanta viruses by polymerase chain reaction techniques and nucleotide sequence analysis. Virus Research 16:127–136
    [Google Scholar]
  16. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  17. Hedman K., Brummer-Korvenkontio M., Vaheri A. 1991; Rapid diagnosis of hanta virus disease by an avidity assay for IgG antibodies. Lancet (in press)
    [Google Scholar]
  18. Isegawa Y., Fujirawa Y., Ohshima A., Fukunaga R., Murakami H., Yamanishi K., Sokawa Y. 1990; Nucleotide sequence of the M genome segment of hemorrhagic fever with renal syndrome virus strain B-l. Nucleic Acids Research 18:4936
    [Google Scholar]
  19. Karabatsos N. (editor) 1985 International Catalogue of Arboviruses 3rd edn San Antonio: American Society of Tropical Medicine and Hygiene;
    [Google Scholar]
  20. Kinnunen L., Huovilainen A., Pöyry T., Hovi T. 1990; Rapid molecular evolution of wild-type 3 poliovirus during infection in individual hosts. Journal of General Virology 71:317–324
    [Google Scholar]
  21. Lähdevirta J., Savola J., Brummer-Korvenkontio M., Berndt R., Illikainen R., Vaheri A. 1984; Clinical and serological diagnosis of nephropathia epidemica, the mild type of haemorrhagic fever with renal syndrome. Journal of Infection 9:230–238
    [Google Scholar]
  22. Lee H. W., Lee P. W. 1976; Korean hemorrhagic fever. I. Demonstration of causative antigen and antibodies. Korean Journal of Internal Medicine 19:371–394
    [Google Scholar]
  23. Lee H. W., Lee P. W., Johnson K. M. 1978; Isolation of the etiologic agent of Korean hemorrhagic fever. Journal of Infectious Diseases 137:298–308
    [Google Scholar]
  24. Lee H. W., Lee P. W., Baek L. J., Chu Y. K. 1990; Geographical distribution of hemorrhagic fever with renal syndrome and hanta viruses. Archives of Virology Supplement 15–18
    [Google Scholar]
  25. Parrington M. A., Kang C. Y. 1990; Nucleotide sequence analysis of the S genomic segment of Prospect Hill virus: comparison with the prototype hanta virus. Virology 175:167–175
    [Google Scholar]
  26. Parrington M. A., Lee P.-W., Kang C. Y. 1991; Molecular characterization of the Prospect Hill virus M RNA segment: a comparison with the M RNA segments of other hanta viruses. Journal of General Virology 72:1845–1854
    [Google Scholar]
  27. Ruusala A., Persson R., Schmaljohn C. S., Pettersson R. 1991; Coexpression of the membrane glycoproteins G1 and G2 of Hantaan virus is required for targeting to the Golgi complex. Virology (in press)
    [Google Scholar]
  28. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning. A Laboratory Manual 2nd edn New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  29. Schmaljohn C. S. 1990; Nucleotide sequence of the L genome segment of Hantaan virus. Nucleic Acids Research 18:6738–6740
    [Google Scholar]
  30. Schmaljohn C. S., Hasty S. E., Dalrymple J. M., Leduc J. W., Lee H. W., von Bonsdorff C.-H., Brummer-Korvenkontio M., Vaheri A., Tsai T. F., Regnery H. L., Goldgaber D., Lee P. W. 1985; Antigenic and genetic properties of viruses linked to hemorrhagic fever with renal syndrome. Science 227:1041–1044
    [Google Scholar]
  31. Schmaljohn C. S., Jennings G. B., Hay J., Dalrymple J. M. 1986; Coding strategy of the S genome segment of Hantaan virus. Virology 155:633–643
    [Google Scholar]
  32. Schmaljohn C. S., Schmaljohn A. L., Dalrymple J. M. 1987; Hantaan virus M RNA: coding strategy, nucleotide sequence and gene order. Virology 157:31–39
    [Google Scholar]
  33. Schmaljohn C. S., Arikawa J., Hasty S. E., Rasmussen L., Lee H. W., Lee P. W., Dalrymple J. M. 1988; Conservation of antigenic properties and sequences encoding the envelope proteins of prototype Hantaan virus and two isolates from Korean haemorrhagic fever patients. Journal of General Virology 69:1949–1955
    [Google Scholar]
  34. Shirako Y., Niklasson B., Dalrymple J. M., Strauss E. G., Strauss J. H. 1991; Structure of the Ockelbo virus genome and its relationship to other Sindbis viruses. Virology 182:753–764
    [Google Scholar]
  35. Stanley K. K., Luzio J. P. 1984; Construction of a new family of high efficiency bacterial expression vectors: identification of cDNA clones coding for human liver proteins. EMBO Journal 3:1429–1434
    [Google Scholar]
  36. Stohwasser R., Giebel L. B., Zöller L., Bautz E. K. F., Darai G. 1990; Molecular characterization of the RNA S segment of nephropathia epidemica virus strain Hällnäs Bl. Virology 174:79–86
    [Google Scholar]
  37. Stohwasser R., Raab K., Darai G., Bautz E. K. F. 1991a; Primary structure of the large (L) RNA segment of nephropathia epidemica virus strain Hällnäs Bl coding for the viral RNA polymerase. Virology 183:386–391
    [Google Scholar]
  38. Stohwasser R., Giebel L. B., Raab K., Bautz E. K. F., Darai G. 1991b; Polymerase chain reaction for detection of hanta viruses. In Frontiers in Virology, vol. 1, Diagnosis of Human Viruses, Polymerase Chain Reaction (PCR) Technology Edited by Becker Y., Darai G. Berlin, Heidelberg, New York & Tokyo: Springer-Verlag; (in press)
    [Google Scholar]
  39. Tang Y. W., Ruo S. L., Sanchez A., Fischer-Hoch S. P., McCormick J. B., Xu Z. Y. 1990; Hantavirus strains isolated from rodentia and insectivora in rural China differentiated by polymerase chain reaction assay. Archives of Virology 115:37–46
    [Google Scholar]
  40. Tkachenko E. A., Bashkiritsev V. N., Van der Groen G., Dzagurova T. K., Ivanov A. P., Ryltseva E. V. 1984; Isolation in Vero E6 cells of hanta virus from Clethrionomys glareolus captured in Bashkiria area of the USSR. Annales de la Societe Beige de Medecine Tropicale 65:121–135
    [Google Scholar]
  41. Yanagihara R., Goldgaber D., Lee P. W., Amyx H. L., Gajdusek D. C., Gibbs C. J. Jr, Svedmyr A. 1984; Propagation of nephropathia epidemica virus in cell culture. Lancet i:1013
    [Google Scholar]
  42. Yoo D., Kang C. Y. 1987; Nucleotide sequence of the M segment of the genomic RNA of Hantaan virus 76-118. Nucleic Acids Research 15:6299–6300
    [Google Scholar]
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