Protoplasts isolated from tomato, wild tomato, barley and chrysanthemum were electrotransfected with tobacco mosaic virus (TMV) RNA under almost the same optimum electric conditions: five square DC pulses of 50 µs duration at 500 to 800 V/cm, with the protoplasts suspended at 2 × 10/ml in 0.5 -mannitol containing 100 µ-MgCl and 10 to 20 µg/ml TMV RNA. ELISAs of these transfected protoplasts showed that the yields and the growth curves of the virus were quite similar, indicating a lack of host specificity in the initially infected cells of these plants.


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