
Full text loading...
Protoplasts isolated from tomato, wild tomato, barley and chrysanthemum were electrotransfected with tobacco mosaic virus (TMV) RNA under almost the same optimum electric conditions: five square DC pulses of 50 µs duration at 500 to 800 V/cm, with the protoplasts suspended at 2 × 105/ml in 0.5 m-mannitol containing 100 µm-MgCl2 and 10 to 20 µg/ml TMV RNA. ELISAs of these transfected protoplasts showed that the yields and the growth curves of the virus were quite similar, indicating a lack of host specificity in the initially infected cells of these plants.
Article metrics loading...
Full text loading...
References
Data & Media loading...