1887

Abstract

The complete nucleotide sequence of the large (L) protein gene of simian virus 5 (SV5) was determined from cDNA of the genomic RNA and mRNA, and found to be 6804 bases in length, exclusive of a poly(A) tract. The sequence contained an open reading frame of 6765 nucleotides encoding 2255 amino acids. Results of dot matrix comparisons of the L protein of SV5 with those of human parainfluenza type 3 virus and Sendai virus indicated that there are five conserved domains, and that each domain contains characteristic sequence(s). The L protein of SV5 was detected in purified virions using antiserum directed against an oligopeptide corresponding to the N-terminal region.

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1992-04-01
2024-12-05
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References

  1. Galinski M. S., Mink M. A., Pons M. W. 1988; Molecular cloning and sequence analysis of the human parainfluenza 3 virus gene encoding the L protein. Virology 165:499–510
    [Google Scholar]
  2. Goswami K. K. A., Lange L. S., Mitchell D. N., Cameron K. R., Russell W. C. 1984; Does simian virus 5 infect humans?. Journal of General Virology 65:1295–1303
    [Google Scholar]
  3. Hamaguchi M., Yoshida T., Nishikawa K., Naruse H., Nagai Y. 1983; Transcriptive complex of Newcastle disease virus. I. Both L and P proteins are required to constitute an active complex. Virology 128:105–117
    [Google Scholar]
  4. Hiebert S. W., Paterson R. G., Lamb R. A. 1985; Hemagglu-tinin-neuraminidase protein of the paramyxovirus simian virus 5: nucleotide sequence of the mRNA predicts an N-terminal mem-brane anchor. Journal of Virology 54:1–6
    [Google Scholar]
  5. Ito Y., Tsurudome M., Hishiyama M., Yamada A. 1987; Immunological interrelationships among human and non-human paramyxoviruses revealed by immunoprecipitation. Journal of General Virology 68:1289–1297
    [Google Scholar]
  6. Kamer G., Argos P. 1984; Primary structural comparison of RNA-dependent polymerases from plant, animal and bacterial viruses. Nucleic Acids Research 12:7269–7282
    [Google Scholar]
  7. Kawano M., Bando H., Yuasa T., Kondo K., Tsurudome M., Komada H., Nishio M., Ito Y. 1990a; Sequence determination of the hemagglutinin-neuraminidase (HN) gene of human para-influenza type 2 virus and the construction of a phylogenetic tree for HN proteins of all the paramyxoviruses that are infectious to humans. Virology 174:308–313
    [Google Scholar]
  8. Kawano M., Bando H., Ohgimoto S., Okomato K., Kondo K., Tsurudome M., Nishio M., Ito Y. 1990b; Complete nucleotide sequence of the matrix gene of human parainfluenza type 2 virus and expression of the M protein in bacteria. Virology 179:857–861
    [Google Scholar]
  9. Kawano M., Okamoto K., Bando H., Kondo K., Tsurudome M., Komada H., Nishio M., Ito Y. 1991; Characterizations of the human parainfluenza 2 virus gene encoding the L protein and the intergenic sequences. Nucleic Acids Research 19:2739–2776
    [Google Scholar]
  10. Kondo K., Bando H., Tsurudome M., Kawano M., Nishio M., Ito Y. 1990; Sequence analysis of the phosphoprotein (P) genes of human parainfluenza type 4A and 4B viruses and RNA editing at transcript of the P genes: the number of G residues added is imprecise. Virology 178:321–326
    [Google Scholar]
  11. Paterson R. G., Harris T. J., Lamb R. A. 1984; Fusion protein of the paramyxovirus simian virus 5: nucleotide sequence of mRNA predicts a highly hydrophobic glycoprotein. Proceedings of the National Academy of Sciences, U.S.A 81:6706–6710
    [Google Scholar]
  12. Poch O., Blumberg B. M., Bougueleret L., Tordo N. 1990; Sequence comparison of five polymerases (L proteins) of unsegmented negative-strand RNA viruses: theoretical assignment of functional domains. Journal of General Virology 71:1153–1162
    [Google Scholar]
  13. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  14. Sheshberadaran H., Lamb R. A. 1990; Sequence characterization of the membrane protein gene of paramyxovirus simian virus 5. Virology 176:234–243
    [Google Scholar]
  15. Shioda T., Iwasaki K., Shibuta H. 1986; Determination of the complete nucleotide sequence of the Sendai virus genome RNA and the predicted amino acid sequence of the F, HN and L proteins. Nucleic Acids Research 14:1545–1563
    [Google Scholar]
  16. Spriggs M. K., Johnson P. R., Collins P. L. 1987; Sequence analysis of the matrix protein gene of human parainfluenza virus type 3: extensive sequence homology among paramyxoviruses. Journal of General Virology 68:1491–1497
    [Google Scholar]
  17. Thomas S. M., Lamb R. A., Paterson R. G. 1988; Two mRNAs that differ by two nontemplated nucleotides encode the amino coterminal proteins P and V of the paramyxovirus SV5. Cell 54:891–902
    [Google Scholar]
  18. Tsurudome M., Nishio M., Komada H., Bando H., Ito Y. 1989; Extensive antigenic diversity among human parainfluenza type 2 virus isolates and immunological relationships among paramyxoviruses revealed by monoclonal antibodies. Virology 171:38–48
    [Google Scholar]
  19. Tsurudome M., Bando H., Nishio M., Iwamoto Y., Kawano M., Kondo K., Komada H., Ito Y. 1990; Antigenic and structural properties of a paramyxovirus simian virus 41 (SV41) reveal a close relationship with human parainfluenza type 2 virus. Virology 179:738–748
    [Google Scholar]
  20. Tsurudome M., Oki N., Higuchi Y., Miyahara K., Yoshimoto M., Mutsuga N., Kitada S., Ogawa M., Miyahara Y., Okamoto K., Kawano M., Komada H., Matsumura H., Kusagawa S., Nishio M., Ito Y. 1991; Molecular relationships between human parainfluenza virus type 2, and simian viruses 41 and 5: determination of nucleoprotein gene sequences of simian viruses 41 and 5. Journal of General Virology 72:2289–2292
    [Google Scholar]
  21. Vidal S., Curran J., Kolakofsky D. 1990; Editing of the Sendai virus P/C mRNA by G insertion occurs during mRNA synthesis via a virus–encoded activity. Journal of Virology 64:239–246
    [Google Scholar]
  22. Yusoff K., Miller S. N., Chambers P., Emmerson P. T. 1987; Nucleotide sequence analysis of the L gene of Newcastle disease virus: homologies with Sendai and vesicular stomatitis viruses. Nucleic acids Research 15:3961–3976
    [Google Scholar]
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