1887

Abstract

The nucleotide sequences of the F and M2 mRNAs of strain A51908 of bovine respiratory syncytial virus (BRSV) were determined by sequencing cDNA of an intracellular dicistronic mRNA. Comparison of the F mRNA sequence with those of other BRSV strains showed that there was extensive sequence identity at both the nucleotide (95% identity) and amino acid (94% identity) levels. Alignment of the nucleotide and encoded amino acid sequences of M2 mRNA of BRSV with those of human respiratory syncytial virus (HRSV) M2 mRNA showed 69% identity at the nucleotide level and 80% identity at the amino acid level. The general features of BRSV F and M2 proteins are similar to those described previously for the HRSV proteins. The M2 mRNA of BRSV also contained a second internal, overlapping open reading frame (ORF) similar to one reported for HRSV. The predicted products of the second ORFs of BRSV and HRSV shared 43% amino acid identity. As described for HRSV, the 3′-terminal end of the M2 mRNA overlaps with the 5′ end of the L gene by 68 nucleotides. The identity between the N-terminal regions of the L proteins of BRSV and HRSV is 75%. In addition, the intergenic sequence of the F-M2 gene junction of BRSV was determined.

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1992-03-01
2024-12-03
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References

  1. Baybutt H. N., Pringle C. R. 1987; Molecular cloning and sequencing of the F and 22K membrane protein genes of the RSS-2 strain of respiratory syncytial virus. Journal of General Virology 68:2789–2796
    [Google Scholar]
  2. Bohlendbr R. E., McCune M. W., Frey M. L. 1982; Bovine respiratory syncytial virus infection. Modern Veterinary Practice 63:613–618
    [Google Scholar]
  3. Collins P. L., Wertz G. W. 1985; The envelope-associated 22K protein of human respiratory syncytial virus: nucleotide sequence of the mRNA and a related polytranscript. Journal of Virology 54:65–71
    [Google Scholar]
  4. Collins J. K., Teegarden R. M., MacVean D. W., Smith G. H., Frank G. R., Salman S. 1988; Prevalence and specificity of antibodies to bovine respiratory syncytial virus in sera from feedlot and range cattle. American Journal of Veterinary Research 49:1316–1319
    [Google Scholar]
  5. Collins P. L., Dickens L. E., Buckler-White A., Olmsted R. A., Spriggs M. K., Camargo E., Coelingh K. V. W. 1986; Nucleotide sequence for the junctions of human respiratory syncytial virus reveal distinctive features of intergenic structure and gene order. Proceedings of the National Academy of Sciences, U.S.A 83:4594–4598
    [Google Scholar]
  6. Collins P. L., Olmsted R. A., Spriggs M. K., Johnson P. R., Buckler-White A. J. 1987; Gene overlap and site-specific attenuation of transcription of the viral polymerase L gene of human respiratory syncial virus. Proceedings of the National Academy of Sciences, U.S.A 84:5134–5138
    [Google Scholar]
  7. Collins P. L., Hill M. G., Johnson P. R. 1990; The two open reading frames of the 22K mRNA of human respiratory syncytial virus: sequence comparison of antigenic subgroups A and B and expression in vitro . Journal of General Virology 71:3015–3020
    [Google Scholar]
  8. Elango N., Satake M., Venkatesan S. 1985; mRNA sequence of three respiratory syncytial virus genes encoding two nonstructural proteins and a 22K structural protein. Journal of Virology 55:101–110
    [Google Scholar]
  9. Galinski M. S. 1991; Paramyxoviridae: transcription and replication. In Advances in Virus Research vol 39 pp 129–162 Edited by Maramorosch K., Murphy F. A. San Diego: Academic Press;
    [Google Scholar]
  10. Johnson P. R., Collins P. L. 1988; The fusion glycoproteins of human respiratory syncytial virus of subgroups A and B: sequence conservation provides a structural basis for antigenic relatedness. Journal of General Virology 69:2623–2628
    [Google Scholar]
  11. Lerch R. A., Stott E. J., Wertz G. W. 1989; Characterization of bovine respiratory syncytial virus proteins and mRNAs and generation of cDNA clones to the viral mRNAs. Journal of Virology 63:833–840
    [Google Scholar]
  12. Lerch R. A., Anderson K., Wertz G. W. 1990; Nucleotide sequence analysis and expression from recombinant vectors demonstrate that the attachment protein G of bovine respiratory syncytial virus is distinct from that of human respiratory syncytial virus. Journal of Virology 64:5559–5569
    [Google Scholar]
  13. Lerch R. A., Anderson K., Amann V. L., Wertz G. W. 1991; Nucleotide sequence analysis of the bovine respiratory syncytial virus fusion protein mRNA and expression from a recombinant vaccinia virus. Virology 181:118–131
    [Google Scholar]
  14. Lopez J. A., Villanueva N., Melero J. A., Portela A. 1988; Nucleotide sequence of the fusion and phosphoprotein genes of human respiratory syncytial (RS) virus Long strain: evidence of subtype genetic heterogeneity. Virus Research 10:249–262
    [Google Scholar]
  15. Mallipeddi S. K., Samal S. K., Mohanty S. B. 1990; Analysis of polypeptides synthesized in bovine respiratory syncytial virus infected cells. Archives of Virology 115:23–36
    [Google Scholar]
  16. Nicholas J. A., Rubino K. L., Levely M. E., Adams E. G., Collins P. L. 1990; Cytolytic Tlymphocyte responses to respiratory syncytial virus: effector cell phenotype and target proteins. Journal of Virology 64:4232–4241
    [Google Scholar]
  17. Openshaw P. J. M., Anderson K., Wertz G. W., Askonas B. A. 1990; The 22,000-kilodalton protein of respiratory syncytial virus is a major target for Kd-restricted cytotoxic T lymphocytes from mice primed by infection. Journal of Virology 64:1683–1689
    [Google Scholar]
  18. Örvell C., Norrby E., Mufson M. A. 1987; Preparation and characterization of monoclonal antibodies directed against five structural components of human respiratory syncytial virus subgroup B. Journal of General Virology 68:3125–3135
    [Google Scholar]
  19. Paccaud M. F., Jacquier C. 1970; A respiratory syncytial virus of bovine origin. Archiv fur die gesamte Virusforschung 30:327–342
    [Google Scholar]
  20. Rose G. D., Roy S. 1980; Hydrophobic basis of packing in globular proteins. Proceedings of the National Academy of Sciences, U.S.A 77:4643–4647
    [Google Scholar]
  21. Samal S. K., Zamora M. 1991; Nucleotide sequence analysis of a matrix and small hydrophobic protein dicistronic mRNA of bovine respiratory syncytial virus demonstrates extensive sequence divergence of the small hydrophobic protein from that of human respiratory syncytial virus. Journal of General Virology 72:1715–1720
    [Google Scholar]
  22. Samal S. K., Zamora M., McPhillips T. H., Mohanty S. B. 1991; Molecular cloning and sequence analysis of bovine respiratory syncytial virus mRNA encoding the major nucleocapsid protein. Virology 180:453–456
    [Google Scholar]
  23. Stott E. J., Taylor G. 1985; Respiratory syncytial virus: a brief review. Archives of Virology 84:1–52
    [Google Scholar]
  24. Taylor G., Stott E. J., Bew M., Fernie B. F., Cote P. J., Collins A. P., Hughes M., Jebbett J. 1984; Monoclonal antibodies protect against respiratory syncytial virus infection in mice. Immunology 52:137–142
    [Google Scholar]
  25. Walravens K., Kettmann R., Collard A., Coppe P., Burny A. 1990; Sequence comparison between the fusion protein of human and bovine respiratory syncytial viruses. Journal of General Virology 71:3009–3014
    [Google Scholar]
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