1887

Abstract

Guinea-pigs were challenged with homologous or heterologous strains of foot-and-mouth disease virus (FMDV) following vaccination with baby hamster kidney (BHK) monolayer cell-adapted or BHK suspension cell-adapted strains of FMDV serotype A22 Iraq 24/64. The protection afforded by these vaccines was analysed as a function of antigen dose and the serum virus neutralization titres achieved. The results show that the level of neutralizing antibody induced that afforded 50% protection was similar for both vaccines in homologous or heterologous challenge situations. However, although the dose of antigen required to achieve this titre against homologous virus was similar for the two vaccines, approximately 20-fold more of the suspension cell-adapted virus was required to elicit a protective titre against heterologous challenge compared to the dose of monolayer cell-adapted virus required. A synthetic peptide representing the amino acid sequence 135 to 167 of VP1, which is identical in the A22 Iraq 24/64 variant viruses, was shown to induce protection against both homologous and heterologous virus challenge.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-3-727
1992-03-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/3/JV0730030727.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-3-727&mimeType=html&fmt=ahah

References

  1. Acharya R., Fry E., Stuart D., Fox G., Rowlands D., Brown F. 1989; The three dimensional structure of foot and mouth disease virus at 2.9Å resolution. Nature, London 327:709–716
    [Google Scholar]
  2. Arrowsmith A. E. M. 1975; Variation among strains of type A foot- and-mouth disease virus in the Eastern Mediterranean region 1964–1972. Journal of Hygiene 75:387–97
    [Google Scholar]
  3. Bahnemann H. G. 1973; The inactivation of FMDV by ethyleneimine and propyleneimine. Zentralblatt fur Veterinaermedizin Reihe B 20:356–360
    [Google Scholar]
  4. Baxt B., Becker Y. 1990; The effect of peptides containing the arginine-glycine-aspartic acid sequence on the adsorption of foot and mouth disease virus to tissue culture cells. Virus Genes 4:73–80
    [Google Scholar]
  5. Bittle J. L., Houghten R. A., Alexander H., Shinnick T. M., Sutcliffe J. G., Lerner R. A., Rowlands D. J., Brown F. 1982; Protection against foot-and-mouth disease by immunisation with a chemically synthesised peptide predicted from the viral nucleotide sequence. Nature, London 298:30–33
    [Google Scholar]
  6. Black L., Pullen L., Boge A. 1985; Guinea pig protection test as indicator of potency of oil emulsion foot and mouth disease vaccines. Research in Veterinary Science 39:207–211
    [Google Scholar]
  7. Bolwell C., Brown A. L., Barnett P. V., Campbell R. O., Clarke B. E., Parry N. R., Ouldridge E. J., Brown F., Rowlands D. J. 1989a; Host cell selection of antigenic variants of foot-and-mouth disease virus. Journal of General Virology 70:45–57
    [Google Scholar]
  8. Bolwell C., Clarke B. E., Parry N. R., Ouldridge E. J., Brown F., Rowlands D. J. 1989b; Epitope mapping of foot and mouth disease virus with neutralizing monoclonal antibodies. Journal of General Virology 70:59–68
    [Google Scholar]
  9. Brown F., Cartwright B. 1963; Purification of radioactive foot- and-mouth disease virus. Nature, London 199:1168–1170
    [Google Scholar]
  10. Capstick P. B., Garland A. J., Masters R. C., Chapman W. G. 1966; Some functional and morphological alterations occurring during and after the adaptation of BHK 21 clone 13 cells to suspension culture. Experimental Cell Research 44:119–128
    [Google Scholar]
  11. Cox D. R. 1989 Analysis of Binary Data Edited by Cox D. R., Snell E. J. London: Chapman and Hall;
    [Google Scholar]
  12. Fox G., Parry N. R., Barnett P. V., McGinn B., Rowlands D. J., Brown F. 1989; The cell attachment site on foot and mouth disease virus includes the amino acid sequence RGD (arginine-glycine-aspartic acid). Journal of General Virology 70:625–637
    [Google Scholar]
  13. Francis M. J., Fry C. M., Rowlands D. J., Brown F., Bittle J. L., Houghten R. A., Lerner R. A. 1985; Immunological priming with synthetic peptides of foot-and-mouth disease virus. Journal of General Virology 66:2347–2354
    [Google Scholar]
  14. Parry N., Fox G., Rowlands D., Brown F., Fry E., Acharya R., Logan D., Stuart D. 1990; Structural and serological evidence for a novel mechanism of antigenic variation in foot and mouth disease virus. Nature, London 347:569–572
    [Google Scholar]
  15. Pay T. W. F., Hingley P. J. 1986; The use of serum neutralization antibody assay for the determination of the potency of foot and mouth disease vaccines in cattle. Developments in Biological Standardization 64:153–161
    [Google Scholar]
  16. Rweyemamu M. M., Booth J. C., Head M., Pay T. W. F. 1978; Microneutralization tests for serological typing and subtyping of foot-and-mouth disease virus strains. Journal of Hygiene 81:107–123
    [Google Scholar]
  17. Rweyemamu M. M., Ouldridge E. J., Head M., Purse F. 1984; Evaluation of the antigenic variation within type A foot-and-mouth disease virus isolates from Asia. Journal of Biological Standardization 12:191–194
    [Google Scholar]
  18. Surovoi A. Y., Ivanov V. T., Chepurkin A. V., Ivanyushchenkov V. N., Dryagalin N. N. 1989; Is the Arg-Gly-Asp sequence the binding site of foot and mouth disease virus with the cell receptor?. Soviet Journal of Bio-Organic Chemistry 14:572–580
    [Google Scholar]
  19. Thomas A. A. M., Woortmeijer R. K., Puijk W., Barteling S. J. 1988; Antigenic sites on foot and mouth disease virus type A10. Journal of Virology 62:2782–2789
    [Google Scholar]
  20. Williams D. A. 1986; Interval estimation of the median lethal dose. Biometrics 42:641–645
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-3-727
Loading
/content/journal/jgv/10.1099/0022-1317-73-3-727
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error