RT Journal Article SR Electronic(1) A1 Ghiasi, Homayon A1 Kaiwar, Ravi A1 Nesburn, Anthony B. A1 Wechsler, Steven L.YR 1992 T1 Baculovirus-expressed glycoprotein H of herpes simplex virus type 1 (HSV-1) induces neutralizing antibody and delayed type hypersensitivity responses, but does not protect immunized mice against lethal HSV-1 challenge JF Journal of General Virology, VO 73 IS 3 SP 719 OP 722 DO https://doi.org/10.1099/0022-1317-73-3-719 PB Microbiology Society, SN 1465-2099, AB We have shown previously that herpes simplex virus type 1 (HSV-1) glycoprotein H (gH) expressed by a baculovirus recombinant is transported to the cell surface in the absence of other HSV-1 gene products, and that the expressed gH has an apparent M r similar to that of authentic HSV-1 gH. We report here that antibodies raised in mice to this baculovirus-expressed gH neutralize the infectivity of HSV-1 in vitro; this neutralizing activity was not complement-dependent. Mice vaccinated with gH also developed delayed type hypersensitivity (DTH) to HSV-1. This is the first report of expressed HSV-1 gH inducing neutralizing antibody or DTH responses in vaccinated animals. In contrast to the gH expressed in mammalian systems, the ability of this baculovirus-expressed gH to induce a neutralizing antibody response may be due to the inability of the mammalian expression system to transport gH to the cell surface. Despite inducing anti-HSV-1 neutralizing antibody and DTH responses, vaccination of mice with gH did not protect the mice against lethal intraperitoneal challenge with HSV-1., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-73-3-719