1887

Abstract

We have synthesized and mapped a cDNA library representing the one major dsRNA element associated with hypovirulence in strain NB58 of the chestnut blight fungus, , which was isolated from recovering chestnut trees in New Jersey, U.S.A. The linear dsRNA has a size of approximately 12.5 kbp and is polyadenylated at the 3′ terminus of one strand. Molecular hybridization experiments indicate that there is sequence similarity between the NB58 dsRNA and dsRNAs from European isolates of , but not among dsRNAs of NB58 and those associated with other North American isolates. Hybridization experiments with mapped cDNA clones representing different regions of the 12.5 kbp dsRNA indicate that the termini and the 3′-proximal two-thirds (relative to the plus strand) are more conserved among NB58 and the European isolates than the rest of the 5′-proximal one-third. Nucleotide sequence analysis of the termini of NB58 dsRNA suggests common organizational features between it and the dsRNA from French-derived strain EP713.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-3-681
1992-03-01
2022-01-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/3/JV0730030681.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-3-681&mimeType=html&fmt=ahah

References

  1. Anagnostakis S. L. 1981; Stability of double-stranded RNA components of Endothia parasitica through transfer and subculture. Experimental Mycology 5:236–242
    [Google Scholar]
  2. Anagnostakis S. L. 1987; Chestnut blight: the classical problem of an introduced pathogen. Mycologia 79:23–37
    [Google Scholar]
  3. Anagnostakis S. L., Day P. R. 1979; Hypovirulence conversion in Endothia parasitica . Phytopathology 69:1226–1229
    [Google Scholar]
  4. Choi G. H., Pawlyk D. M., Nuss D. L. 1991a; The autocatalytic protease p29 encoded by a hypovirulence-associated virus of the chestnut blight fungus resembles the potyvirus-encoded protease HC-Pro. Virology 183:747–752
    [Google Scholar]
  5. Choi G. H., Shapira R., Nuss D. L. 1991b; Co-translational autoproteolysis involved in gene expression from a double-stranded RNA genetic element associated with hypovirulence of the chestnut blight fungus. Proceedings of the National Academy of Sciences, U.S.A 88:1167–1171
    [Google Scholar]
  6. Day P. R., Dodds J. A., Elliston J. E., Jaynes R. A., Anagnostakis S. L. 1977; Double-stranded RNA in Endothia parasitica . Phytopathology 67:1393–1396
    [Google Scholar]
  7. Dodds J. A. 1980; Association of type 1 viral dsRNA with clubshaped particles in hypovirulent strains of Endothia parasitica . Virology 107:1–12
    [Google Scholar]
  8. Fulbright D. W. 1984; Effect of eliminating dsRNA in hypovirulent Endothia parasitica . Phytopathology 74:722–724
    [Google Scholar]
  9. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  10. Hansen D. R., Van Alfen N. K., Gillies K., Powell W. A. 1985; Naked dsRNA associated with hypovirulence of Endothia parasitica is packaged in fungal vesicles. Journal of General Virology 66:2605–2614
    [Google Scholar]
  11. Hillman B. I., Hearne P., Rochon D., Morris T. J. 1989a; Organization of tomato bushy stunt virus genome: characterization of the coat protein and the 3′ terminus. Virology 169:42–50
    [Google Scholar]
  12. Hillman B. I., Rae B. P., Tartaglia J., Nuss D. L. 1989b; Elucidating the structure and function of double-stranded RNA genetic elements associated with biological control of chestnut blight. In Molecular Biology of Plant-Pathogen Interaction . Proceedings of the UCLA Symposium 101:59–70
    [Google Scholar]
  13. Hillman B. I., Shapira R., Nuss D. L. 1990; Hypovirulence-associated suppression of host functions in Cryphonectria parasitica can be partially relieved by high light intensity. Phytopathology 80:950–956
    [Google Scholar]
  14. Hiremath S., L’Hostis B., Ghabrial S. A., Rhoads R. E. 1986; Terminal structure of hypovirulence-associated dsRNAs in the chestnut blight fungus Endothiaparasitica . Nucleic Acids Research 14:9877–9896
    [Google Scholar]
  15. Hiremath S., Nuss D., Ghabrial S. A., Rhoads R. E. 1988; Molecular cloning of hypovirulence-associated double-stranded RNA in Endothia parasitica and detection of sequence-related single-stranded RNA. Journal of General Virology 69:2441–2453
    [Google Scholar]
  16. Holmes D. S., Quigley M. 1981; A rapid boiling method for the preparation of bacterial plasmids. Analytical Biochemistry 114:193–197
    [Google Scholar]
  17. Kozak M. 1989; The scanning model for translation: an update. Journal of Cell Biology 108:229–241
    [Google Scholar]
  18. L’Hostis B., Hiremath S. T., Rhoads R. E., Ghabrial S. A. 1985; Lack of sequence homology between double-stranded RNA from European and American hypovirulent strains of Endothia parasitica . Journal of General Virology 66:351–355
    [Google Scholar]
  19. Morris T. J., Dodds J. A. 1979; Isolation and analysis of double-stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69:854–858
    [Google Scholar]
  20. Paul C. P., Fulbright D. W. 1988; Double-stranded RNA molecules from Michigan hypovirulent isolates of Endothiaparasitica vary in size and sequence homology. Phytopathology 78:751–755
    [Google Scholar]
  21. Queen C., Korn L. J. 1984; A comprehensive sequence analysis program for the IBM personal computer. Nucleic Acids Research 12:581–599
    [Google Scholar]
  22. Rae B. P., Hillman B. I., Tartaglia J., Nuss D. L. 1989; Characterization of double-stranded RNA genetic elements associated with biological control of chestnut blight: organization of terminal domains and identification of gene products. EMBO Journal 8:657–663
    [Google Scholar]
  23. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  24. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  25. Shapira R., Choi G. H., Hillman B. I., Nuss D. L. 1991a; The contribution of defective RNAs to the complexity of viral-encoded double-stranded RNA populations present in hypovirulent strains of the chestnut blight fungus, Cryphonectria parasitica . EMBO Journal 10:741–746
    [Google Scholar]
  26. Shapira R., Choi G. H., Nuss D. L. 1991b; Virus-like genetic organization and expression strategy for a double-stranded RNA genetic element associated with biological control of chestnut blight. EMBO Journal 10:731–739
    [Google Scholar]
  27. Tartaglia J., Paul C. P., Fulbright D. W., Nuss D. L. 1986; Structural properties of double-stranded RNAs associated with biological control of chestnut blight fungus. Proceedings of the National Academy of Sciences, U.S.A 83:9109–9113
    [Google Scholar]
  28. Van Alfen N. K. 1982; Biology and potential for disease control of hypovirulence of Endothia parasitica . Annual Review of Phytopathology 20:349–362
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-3-681
Loading
/content/journal/jgv/10.1099/0022-1317-73-3-681
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error