1887

Abstract

RNA of more than 40 hantavirus isolates, originating from rodents and humans of widely separated geographical areas, was copied to cDNA using reverse transcriptase and amplified by polymerase chain reaction (PCR). A genus-reactive oligonucleotide primer pair, flanking a 365 bp region of the G2 glycoprotein gene, was chosen for genus-reactive PCR. DNA products were digested with 20 restriction endonucleases and cleavage patterns were analysed. For strains of known sequence, the restriction patterns observed were consistent with those predicted from sequence data, demonstrating that the amplified products originated from target virus RNA. Further analyses suggested that all amplified viruses could be easily typed into one of five restriction patterns using only five enzymes. The categories identified by restriction analysis of PCR-amplified cDNA corresponded with serogroups established by plaque-reduction neutralization tests. This method may greatly simplify the identification of new hantavirus isolates.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-3-567
1992-03-01
2024-12-13
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/3/JV0730030567.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-3-567&mimeType=html&fmt=ahah

References

  1. Antic D., Lim B.-U., Kang C. Y. 1991; Molecular characterization of the M genomic segment of the Seoul 80-39 virus: nucleotide and amino acid sequence comparisons with other hantaviruses reveal the evolutionary pathway. Virus Research 19:47–57
    [Google Scholar]
  2. Antoniades A., Grekas D., Rossi C. A., LeDuc J. W. 1987; Isolation of a hantavirus from a severely ill patient with hemorrhagic fever with renal syndrome in Greece. Journal of Infectious Diseases 156:1010–1013
    [Google Scholar]
  3. Arikawa J., Lapenotiere H. F., Iacono-Connors L., Wang M., Schmaljohn C. S. 1990; Coding properties of the S and the M genome segments of Sapporo rat virus: comparison to other causative agents of hemorrhagic fever with renal syndrome. Virology 176:114–125
    [Google Scholar]
  4. Baek L. J., Yanagihara R., Gibbs C. J. Jr, Miyazaki M., Gajdusek D. C. 1988; Leakey virus: a new hantavirus isolated from Mus musculus in the United States. Journal of General Virology 69:3129–3132
    [Google Scholar]
  5. Carey D. E., Reuben R., Panicker K. N., Shope R. E., Myers R. M. 1971; Thottapalayam virus: a presumptive arbovirus isolated from a shrew in India. Indian Journal of Medical Research 59:1758–1760
    [Google Scholar]
  6. Childs J., Korch G., Glass G., LeDuc J., Shah K. 1987; Epizootiology of hantavirus infections in Baltimore: isolation of a virus from Norway rats and characteristics of infected rat populations. American Journal of Epidemiology 126:55–68
    [Google Scholar]
  7. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. 1979; Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294–5299
    [Google Scholar]
  8. Chomczynski P., Sacchi N. 1987; Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162:156–159
    [Google Scholar]
  9. Dantas J. R. Jr, Okuno Y., Tanishita O., Takahashi Y., Takahashi M., Kurata T., Lee H. W., Yamanishi K. 1987; Viruses of hemorrhagic fever with renal syndrome (HFRS) grouped by immunoprecipitation and hemagglutination inhibition. Intervirology 27:161–165
    [Google Scholar]
  10. Elwell M. R., Ward G. S., Tingpalapong M., LeDuc J. W. 1985; Serologic evidence of Hantaan-like virus in rodents and man in Thailand. Southeast Asian Journal of Tropical Medicine and Public Health 16:349–354
    [Google Scholar]
  11. Giebel L. B., Stohwasser R., Zöller L., Bautz E. K. F., Darai G. 1989; Determination of coding capacity of the M genome segment of nephropathia epidemica virus strain Hällnas Bl by molecular cloning and nucleotide sequence analysis. Virology 172:498–505
    [Google Scholar]
  12. Gligic A., Obradovic M., Stojanovic R., Vujosevic N., Ovcaric A., Frusic M., Gibbs C. J. Jr, Calisher C. H., Gajdusek D. C. 1989; Epidemic hemorrhagic fever with renal syndrome in Yugoslavia. American Journal of Tropical Medicine and Hygiene 41:102–108
    [Google Scholar]
  13. Kitamura T., Morita C., Komatsu T., Sugiyama K., Arikawa J., Shiga S., Takeda H., Akao Y., Imaizumi K., Oya A., Hashimoto N., Urasawa S. 1983; Isolation of virus causing hemorrhagic fever with renal syndrome (HFRS) through a cell culture system. Japanese Journal of Medical Sciences and Biology 36:17–25
    [Google Scholar]
  14. LeDuc J. W., Smith G. A., Bagley L. R., Hasty S. E., Johnson K. M. 1982; Preliminary evidence that Hantaan or a closely related virus is enzootic in domestic rodents. New England Journal of Medicine 307:624
    [Google Scholar]
  15. LeDuc J. W., Smith G. A., Pinheiro F. P., Vasconcelos P. F. C., Rosa E. S. T., Maiztegui J. I. 1985; Isolation of a Hantaan-related virus from Brazilian rats and serologic evidence of its widespread distribution in South America. American Journal of Tropical Medicine and Hygiene 34:810–815
    [Google Scholar]
  16. LeDuc J. W., Smith G. A., Childs J. E., Pingeiro F. P. Maiztegui J. L., Niklasson B., Antoniades A., Robinson D. M., Khin M., Shortridge K. F., Wooster M. T., Elwell M. R., Ilbery P. L. T., Koech D., Rosa E. S. T., Rosen L. 1986; Global survey of antibody to Hantaan-related viruses among peridomestic rodents. Bulletin of the World Health Organization 64:139–144
    [Google Scholar]
  17. Lee H. W. 1990; Hemorrhagic fever with renal syndrome (Korean hemorrhagic fever). Annual Summary Report, Korea University College of Medicine
    [Google Scholar]
  18. Lee H. W., Lee P. W., Johnson K. M. 1978; Isolation of the etiologic agent of Korean hemorrhagic fever. Journal of Infectious Diseases 137:298–308
    [Google Scholar]
  19. Lee H. W., Lee P. W., Baek L. J., Song C. K., Seong I. W. 1981; Intraspecific transmission of Hantaan virus, etiologic agent of Korean hemorrhagic fever, in the rodent Apodemus agrarius . American Journal of Tropical Medicine and Hygiene 30:1106–1112
    [Google Scholar]
  20. Lee H. W., Baek L. J., Johnson K. M. 1983; Isolation of Hantaan virus, the etiologic agent of Korean hemorrhagic fever from wild urban rats. Journal of Infectious Diseases 146:638–644
    [Google Scholar]
  21. Lee P. W., Gibbs C. J. Jr, Gajdusek D. C., Hsiang C.-M., Hsiung G. D. 1980; Identification of epidemic haemorrhagic fever with renal syndrome in China with Korean haemorrhagic fever. Lancet i:1025–1026
    [Google Scholar]
  22. Lee P. W., Amyx H. L., Gajdusek D. C., Yanagihara R. T., Goldgaber D., Gibbs C. J. Jr 1982; New haemorrhagic fever with renal syndrome-related virus in indigenous wild rodents in United States. Lancet ii:1405
    [Google Scholar]
  23. Lee P. W., Amyx H. L., Yanagihara R., Gajdusek D. C., Goldgaber D., Gibbs C. J. Jr 1985a; Partial characterization of Prospect Hill virus isolated from meadow voles in the United States. Journal of Infectious Diseases 152:826–829
    [Google Scholar]
  24. Lee P. W., Gibbs C. J. Jr, Gajdusek D. C., Yanagihara R. 1985b; Serotypic classification of hantaviruses by indirect immuno-fluorescent antibody and plaque reduction neutralization tests. Journal of Clinical Microbiology 22:940–944
    [Google Scholar]
  25. Ni D.-S., Shen H.-K., Yu R.-H., Zhou L., Zhu C.-F., Zhang S.-Q. 1983; Identification of a strain of epidemic hemorrhagic fever (EHF) virus from patients’s sera. Chinese Medical Journal 63:65–68
    [Google Scholar]
  26. Niklasson B., LeDuc J. W. 1984; Isolation of the nephropathia epidemica agent in Sweden. Lancet i:1012–1013
    [Google Scholar]
  27. Schmaljohn C. S., Dalrymple J. M. 1983; Analysis of Hantaan virus RNA: evidence for a new genus of Bunyaviridae. Virology 131:482–491
    [Google Scholar]
  28. Schmaljohn C. S., Hasty S. E., Dalrymple J. M., LeDuc J. W., Lee H. W., von Bonsdorff C.-H., Brummer-Korvenkontio M., Vaheri A., Tsai T. F., Regnery H. L., Goldgaber D., Lee P.-W. 1985; Antigenic and genetic properties of viruses linked to hemorrhagic fever with renal syndrome. Science 227:1041–1044
    [Google Scholar]
  29. Schmaljohn C. S., Schmaljohn A. L., Dalrymple J. M. 1987; Hantaan virus M RNA: coding strategy, nucleotide sequence, and gene order. Virology 157:31–39
    [Google Scholar]
  30. Schmaljohn C. S., Arikawa J., Hasty S. E., Rasmussen L., Lee H. W., Lee P. W., Dalrymple J. M. 1988; Conservation of antigenic properties and sequences encoding the envelope proteins of prototype Hantaan virus and two virus isolates from Korean haemorrhagic fever patients. Journal of General Virology 69:1949–1955
    [Google Scholar]
  31. Song G. 1982; The etiological studies of epidemic hemorrhagic fever. I. Isolation and characterization of a virus strain using the Apodemus agrarius from the unendemic area. Acta academiae medicinae sinica 4:66–73
    [Google Scholar]
  32. Song G., Hang C.-S., Liao H.-X., Qiu X.-Z., Gao G.-Z., Du Y.-L., Zhao J.-N., Xu J.-K., Kong B.-X. 1982; Isolation of EHF-related agent from Rattus norvegicus captured in patients’ home in endemic area of the mild type of hemorrhagic fever. Acta microbiologica sinica 22:373–377
    [Google Scholar]
  33. Tkachenko E. A., Bashkirtsev V. N., van der Groen G., Dzagurova T. K., Ivanov A. P., Ryltseva E. V. 1984; Isolation in Vero-E6 cells of Hantavirus from Clethrionomys glareolus captured in the Bashkiria area of the U.S.S.R. Annales de la Société beige de médicine tropicale 64:425–426
    [Google Scholar]
  34. Tsai T., Bauer S., Sasso D., Whitfield S., McCormick J., Caraway T., McFarland L., Bradford H., Kurata T. 1985; Serological and virological evidence of a Hantaan virus-related enzootic in the United States. Journal of Infectious Diseases 152:126–136
    [Google Scholar]
  35. Xiao S.-Y., Yanagihara R., Godec M. S., Eldadah Z. A., Johnson B. K., Gajdusek D. C., Asher D. M. 1991; Detection of hantavirus RNA in tissues of experimentally infected mice using reverse transcriptase-directed polymerase chain reaction. Journal of Medical Virology 33:277–282
    [Google Scholar]
  36. Yamanishi K., Domar K., Kawamata J., Kurata T. 1983; Isolation of hemorrhagic fever with renal syndrome (HFRS) virus from a tumor specimen in a rat. Biken Journal 26:155–160
    [Google Scholar]
  37. Yanagihara R., Svedmyr A., Amyx H. L., Lee P., Goldgaber D., Gajdusek D. C., Gibbs C. J. Jr, Nystrom K. 1984; Isolation and propagation of nephropathia epidemica virus in bank voles. Scandinavian Journal of Infectious Diseases 16:225–228
    [Google Scholar]
  38. Yang Z.-Q., Zhu B.-L., Zhang M., Hu Z., Xiao S.-Y., Zhang T., Zheng Z., Xiang J., Huggins J. W. 1988; Isolation and identification of EHFV from plasma and urine of patients of EHF in fever phase. Acta academiae medicinae Hubei 9:301–306
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-73-3-567
Loading
/content/journal/jgv/10.1099/0022-1317-73-3-567
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error