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Previous studies have shown that β 2 microglobulin (β 2m) is associated with glycoproteins present on the envelope of urinary human cytomegalovirus (CMV). β 2m is non-covalently associated with the α chain of human leukocyte antigen (HLA) class I antigens and therefore it was of interest to determine whether the class I α chain is also associated with the β 2m–CMV complex. Using a panel of monoclonal antibodies recognizing different conformational determinants on the HLA class I heterodimer or free β 2m, we have shown that β 2m but not the α chain of HLA class I could be immunoprecipitated from 125I-surface-labelled virions purified directly from urine. We therefore conclude that host class I HLA α chains are not associated with β 2m on the envelope of urinary CMV.
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