Production of the tobacco mosaic virus (TMV) transport protein in transgenic plants is essential but insufficient for complementing foreign virus transport: a need for the full-length TMV genome or some other TMV-encoded product Free

Abstract

We have reported previously that tobamoviruses enable the transport of red clover mottle comovirus (RCMV) in tobacco plants normally resistant to RCMV. Here we show that RCMV transport does not take place in transgenic tobacco plants (line To-4) producing the 30K transport protein of tobacco mosaic virus (TMV), whereas the transport of the TMV Ls1 mutant, the cell-to-cell movement of which is temperature sensitive, is complemented in these plants. However, RCMV transport is observed when these transgenic plants are infected with both RCMV and TMV Ls1 at the non-permissive temperature (33 °C). It is suggested that (i) the hypothetical modification of transgenic plant plasmodesmata by the TMV 30K transport protein can specifically mediate the cell-to-cell movement of the homologous virus (TMV), but is insufficient to mediate RCMV transport; (ii) the presence of the full-length TMV genome or a certain TMV-encoded product(s) besides the 30K protein is essential for complementation of the RCMV transport function. The possibility that line To-4 might provide enough 30K protein to complement TMV Ls1 but not RCMV cannot be ruled out. During double infection the mutant 30K protein may, in concert with the wild-type 30K protein, provide the transport function for RCMV.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-2-471
1992-02-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/2/JV0730020471.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-2-471&mimeType=html&fmt=ahah

References

  1. Atabekov J. G., Taliansky M. E. 1990; Expression of virus-coded transport function by different viral genomes. Advances in Virus Research 38:201–247
    [Google Scholar]
  2. Atkins D., Hull R., Wells B., Roberts K., Moore P., Beachy R. N. 1991; The tobacco mosaic virus 30K movement protein in transgenic tobacco plants is localized to plasmodesmata. Journal of General Virology 72:209–211
    [Google Scholar]
  3. Bevan M. 1984; Binary Agrobacterium vectors for plant transformation. Nucleic Acids Research 12:8711–8721
    [Google Scholar]
  4. Clark M. F., Adams A. N. 1977; Characteristics of the microplate method of enzyme-linked immunosorbent assay for detection of plant viruses. Journal of General Virology 34:475–483
    [Google Scholar]
  5. Deom C. M., Oliver M. J., Beachy R. N. 1987; The 30-kilodalton gene product of tobacco mosaic virus potentiates virus movement. Science 237:389–394
    [Google Scholar]
  6. Deom C. M., Schubert K. R., Wolf S., Holt C. A., Lucas W. J., Beachy R. N. 1990; Molecular characterization and biological function of the movement protein of tobacco mosaic virus in transgenic plants. Proceedings of the National Academy of Sciences, U.S.A 87:3284–3288
    [Google Scholar]
  7. Dorokhov Yu. L., Alexandrova N. M., Miroshnichenko N. A., Atabekov J. G. 1984; The informosome-like virus-specific ribonucleoprotein (vRNP) may be involved in the transport of tobacco mosaic virus infection. Virology 137:127–134
    [Google Scholar]
  8. Horsch R. B., Fry J. E., Hoffman N. L., Eichholtz D., Rogers S. G., Fraley R. T. 1985; A simple and general method for transferring genes into plants. Science 227:1229–1231
    [Google Scholar]
  9. Hull R. 1989; The movement of viruses in plants. Annual Review of Phytopathology 27:213–240
    [Google Scholar]
  10. Malyshenko, L. S., Kondakova O. A., Taliansky M. E., Atabekov J. G. 1989; Plant virus transport function: complementation by helper viruses is non-specific. Journal of General Virology 70:2751–2757
    [Google Scholar]
  11. Meshi T., Watanabe Y., Sugimoto A., Maeda T., Okada Y. 1987; Function of the 30kd protein of tobacco mosaic virus: involvement in cell-to-cell movement and dispensability for replication. EMBO Journal 6:2557–2563
    [Google Scholar]
  12. Meyer M., Hemmer O., Mayo M. A., Fritsch C. 1986; The nucleotide sequence of tomato black ring virus RNA-2. Journal of General Virology 61:1257–1271
    [Google Scholar]
  13. Nishiguchi M., Motoyoshi F., Oshima N. 1978; Behaviour of a temperature sensitive strain of tobacco mosaic virus in tomato leaves and protoplasts. Journal of General Virology 39:53–61
    [Google Scholar]
  14. Nishiguchi M., Motoyoshi F., Oshima N. 1980; Further investigation of a temperature-sensitive strain of tobacco mosaic virus: its behaviour in tomato leaf epidermis. Journal of General Virology 46:497–500
    [Google Scholar]
  15. Rezelman G., Franssen H. J., Goldbach R. W., Ie T. S., van Kammen A. 1982; Limits to the independence of bottom component RNA of cowpea mosaic virus. Journal of General Virology 60:335–342
    [Google Scholar]
  16. Shanks M., Tomenius K., Clapham D., Huskisson N. S., Barker P. J., Maule A. J., Lomonossoff G. P. 1989; Identification and subcellular localization of a putative cell-to-cell transport protein from red clover mottle virus. Virology 173:400–407
    [Google Scholar]
  17. Topfer R., Matzeit V., Gronenborn B., Schell J., Steinbiss H.-H. 1987; A set of plant expression vectors for transcriptional and translational fusions. Nucleic Acids Research 15:5890
    [Google Scholar]
  18. van Lent J., Wellink J., Goldbach R. 1990; Evidence for the involvement of the 58K and 48K proteins in the intercellular movement of cowpea mosaic virus. Journal of General Virology 71:219–223
    [Google Scholar]
  19. Wellink J., van Kammen A. 1989; Cell-to-cell transport of cowpea mosaic virus requires both the 58K/48K proteins and the capsid proteins. Journal of General Virology 70:2279–2286
    [Google Scholar]
  20. Wolf S., Deom C. M., Beachy R. N., Lucas W. J. 1989; Movement protein of tobacco mosaic virus modifies plasmodesmatal size exclusion limit. Science 246:337–379
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-2-471
Loading
/content/journal/jgv/10.1099/0022-1317-73-2-471
Loading

Data & Media loading...

Most cited Most Cited RSS feed