Eight overlapping icosapeptides covering the entire sequence of the E4 protein of human papillomavirus type 16 (HPV-16), were prepared and tested for their reactivity with human sera in IgG-specific ELISA. The strongest reactivity of sera from HPV-16 DNA-positive invasive cervical carcinoma (INCA) patients was detected with the peptide denoted 16/E4-6, covering amino acids 51 to 70. Subsequently nearly 200 sera were tested for the presence of the 16/E4-6-specific antibody. Reactivity was more frequent in cervical intraepithelial neoplasia patients and INCA patients than in matched control subjects. Sera from INCA patients were also tested for antibody reactive with peptide 16/E7-2 covering the major type-specific reactive region of the HPV-16 E7 protein. Only four of 13 sera possessing the 16/E4-6-specific antibody were reactive with the 16/E7-2 peptide.
ChowL. T.,
NasseriM.,
WolinskyS. M.,
BrokerT. R.1987; Human papillomavirus types 6 and 11 m-RNAs from genital condylomata acuminata. Journal of Virology 61:2581–2588
DillnerJ.1990; Mapping of linear epitopes of human papillomavirus type 16: the El, E2, E5, E6 and E7 open reading frames. International Journal of Cancer 46:703–711
DillnerJ.,
DillnerL.,
RobbJ.,
WillensJ.,
JonesI.,
LancasterW.,
SmithR.,
LernerR.1989; A synthetic peptide defines a serologic IgA response to a human papillomavirus-encoded nuclear antigen expressed in virus-carrying cervical neoplasia. Proceedings of the National Academy of Sciences, U.S.A. 86:3838–3841
DoorbarJ.,
ConeronI.,
GallimoreP. M.1989; Sequence divergence yet conserved physical characteristics among the E4 proteins of cutaneous human papillomaviruses. Virology 172:51–62
DürstM.,
Dzarlieva-PetrusevskaR. T.,
BoukampP.,
FusenigN. E.,
GissmanL.1987; Molecular and cytogenetic analysis of immortalized human primary keratinocytes obtained after transfection with human papillomavirus type 16 DNA. Oncogene 1:251–256
JenisonS. A.,
YuX. P.,
ValentineJ. M.,
GallowayD.1989; Human antibodies react with an epitope of the human papillomavirus type 6b L1 open reading frame which is distinct from the typecommon epitope. Journal of Virology 63:809–818
JenisonS. A.,
YuX. P.,
ValentineJ. M.,
KoutskyL. A.,
ChristiansenA. E.,
BeckmannA. M.,
GallowayD.1990; Evidence of prevalent genital-type human papillomavirus infections in adults and children. Journal of Infectious Diseases 162:60–69
JenisonS. A.,
YuX. P.,
ValentineJ. M.,
GallowayD. A.1991; Characterization of human antibody-reactive epitopes encoded by human papillomavirus types 16 and 18. Journal of Virology 65:1208–1218
Jochmus-KudielkaI.,
SchneiderA.,
BraunR.,
KimmigR.,
KoldovskyU.,
SchneweisK. E.,
SeedorfK.,
GissmanL.1989; Antibodies against the human papillomavirus type 16 early proteins in human sera: correlation of anti E7 reactivity with cervical cancer. Journal of the National Cancer Institute 82:1698–1703
KöchelH. G.,
SievertK.,
MonazahianM.,
Mittelstadt-DeterdingA.,
TeichmannA.,
ThomssenR.1991; Antibodies to human papillomavirus type-16 in human sera as revealed by use of prokaryotically expressed viral gene products. Virology 182:644–654
KrchňákV.,
VágnerJ.,
SuchnákováA.,
KrčmářM.,
Ritter-ováL.,
VonkaV.1990; Synthetic peptides derived from E7 region of human papillomavirus type 16 used as antigens in ELISA. Journal of General Virology 71:2719–2724
MannV.,
Loo de LaoS.,
BrenesM.,
BrintonC. A.,
RawlsJ. A.,
GreenM.,
ReevesW. C.,
RawlsW. E.1990; Occurrence of IgA and IgG antibodies to select peptides representing human papillomavirus type 16 among cervical cancer cases and controls. Cancer Research 50:7815–7819
MüllerM.,
GausepohlH.,
de MartynofF. G.,
FrankR.,
BrasseurR.,
GissmannL.1990; Identification of seroreactive regions of the human papillomavirus type 16 proteins E4, E6, E7 and L1. Journal of General Virology 71:2709–2717
SuchánkováA.,
RitterO.,
HirschI.,
KrchňákV.,
KalošZ.,
HamšíkováE.,
BřicháčekB.,
VonkaV.1990; Presence of antibody reactive with synthetic peptide derived from L2 open reading frame of human papillomavirus types 6b and 11 in human sera. Acta virologica 34:433–442
SuchánkováA.,
RitterováL.,
KrčmářM.,
KrchňákV.,
VágnerJ.,
JochmusL.,
GissmannL.,
KaŇkaJ.,
VonkaV.1991; Comparison of ELISA and Western blotting for human papillomavirus type 16 E7 antibody determination. Journal of General Virology 72:2577–2581