1887

Abstract

The VP-2 genes of canine parvovirus (CPV) and a recombinant consisting of CPV and feline panleukopenia virus (FPV) sequences were cloned into baculovirus expression vectors, fused to the baculovirus polyhedrin promoter. Recombinant baculoviruses were prepared and the properties of the parvovirus proteins expressed in insect cells examined. The proteins produced were the same size as the authentic CPV VP-2 protein, and were produced late after infection; the quantity of proteins recovered from the insect cell cultures was similar to those produced in CPV infections. Parvovirus particles formed had the haemagglutination (HA), sedimentation and buoyant density properties of authentic CPV capsids. Both the CPV capsids and the CPV-FPV recombinant capsids from the baculovirus system expressed the same epitopes as those seen in the viable parvoviruses when tested with a panel of anti-parvovirus monoclonal antibodies. Lysates of recombinant baculovirus-infected cells were inoculated into dogs, giving rise to serum neutralizing and HA-inhibiting antibodies, and the immunized dogs were protected from clinical disease upon challenge with a virulent isolate of the most recent antigenic type of CPV.

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1992-02-01
2024-10-08
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References

  1. Bass E. P., Gill M. A., Beckenhauer W. H. 1982; Development of a modified live, canine origin parvovirus vaccine. Journal of the American Veterinary Medical Association 181:909–913
    [Google Scholar]
  2. Brown C. S., Salimans M. M. M., Noteborn M. H. M., Weiland H. T. 1990; Antigenic parvovirus B19 coat proteins VP1 and VP2 produced in large quantities in a baculovirus expression system. Virus Research 15:197–212
    [Google Scholar]
  3. Burand J. P., Summers M. D., Smith G. E. 1980; Transfection with baculovirus DNA. Virology 101:286–290
    [Google Scholar]
  4. Carmichael L. E., Joubert J. C., Pollock R. V. H. 1980; Hemagglutination by canine parvovirus: serologic studies and diagnostic applications. American Journal of Veterinary Research 40:784–791
    [Google Scholar]
  5. Carmichael L. E., Joubert J. C., Pollock R. V. H. 1981; A modified live canine parvovirus strain with novel plaque characteristics. I. Viral attenuation and dog response. Cornell Veterinarian 71:408–427
    [Google Scholar]
  6. Carmichael L. E., Joubert J. C., Pollock R. V. H. 1983; A modified live canine parvovirus vaccine. II. Immune response. Cornell Veterinarian 73:13–29
    [Google Scholar]
  7. Cotmore S. F., Tattersall P. 1987; The autonomously replicating parvoviruses of vertebrates. Advances in Virus Research 33:91–174
    [Google Scholar]
  8. Crandell R. A., Fabricant C. G., Nelson-Rees W. A. 1973; Development, characterization and viral susceptibility of a feline (Fehs catus) renal cell line (CRFK). In Vitro 9:176–185
    [Google Scholar]
  9. Eugster A. K. 1980; Studies on canine parvovirus infections: development of an inactivated vaccine. American Journal of Veterinary Research 41:2020–2024
    [Google Scholar]
  10. French T. J., Marshall J. J. A., Roy P. 1990; Assembly of double-shelled, viruslike particles of bluetongue virus by the simultaneous expression of four structural proteins. Journal of Virology 64:5695–5700
    [Google Scholar]
  11. Hallauer C., Kronauer G., Siegl G. 1971; Parvoviruses as contaminants of permanent human cell lines. I. Virus isolations from 1960–1970. Archiv fur die gesamte Virusforschung 35:80–90
    [Google Scholar]
  12. Hermonat P. L., Labow M. A., Wright R., Berns K. I., Muzyczka N. 1984; Genetics of adeno-associated virus: isolation and preliminary characterization of adeno-associated virus type 2 mutants. Journal of Virology 51:329–339
    [Google Scholar]
  13. Hink W. F. 1970; Established insect cell line from the cabbage looper, Trichoplusia ni. Nature, London 226:466–467
    [Google Scholar]
  14. Kajigaya S., Shimada T., Fujita S., Young N. S. 1989; A genetically engineered cell line that produces empty capsids of B19 (human) parvovirus. Proceedings of the National Academy of Sciences, U.S.A. 86:7601–7605
    [Google Scholar]
  15. Kajigaya S., Fujii H., Field A., Anderson S., Rosenfeld S., Anderson L. J., Shimada T., Young N. S. 1991; Self-assembled B19 parvovirus capsids, produced in a baculovirus system, are antigenically and immunologically similar to native virions. Proceedings of the National Academy of Sciences, U.S.A 88:4646–4650
    [Google Scholar]
  16. Labieniec-Pintel L., Pintel D. 1986; The minute virus of mice P39 transcription unit can encode both capsid proteins. Journal of Virology 57:1163–1167
    [Google Scholar]
  17. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  18. Luckow V. A., Summers M. D. 1988; Trends in the development of baculovirus expression vectors. Bio/Technology 6:47–55
    [Google Scholar]
  19. Luo L., Li Y., Kang C. Y. 1990; Expression of gag precursor protein and secretion of virus like gag particles of HIV-2 from recombinant baculovirus–infected insect cells. Virology 179:874–880
    [Google Scholar]
  20. Mazzara G. P., Destree A. T., Williams H. W., Sue J. M., Belanger L. M., Panicali D., Parrish C. R. 1987; Successful vaccination of dogs with empty capsids derived from canine parvovirus-bovine papillomavirus chimeric plasmids. In Vaccines’87 pp 419–424 Edited by Chanock R. M., Lerner R. A., Brown F., Ginsberg H. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  21. Paradiso P. R., Rhode S. L. III, Singer I. I. 1982; Canine parvovirus: a biochemical and ultrastructural characterization. Journal of General Virology 62:113–125
    [Google Scholar]
  22. Parrish C. R. 1991; Mapping specific functions in the capsid structure of canine parvovirus and feline panleukopenia virus using infectious plasmid clones. Virology 183:195–205
    [Google Scholar]
  23. Parrish C. R., Carmichael L. E. 1983; Antigenic structure and variation of canine parvovirus type-2, feline panleukopenia virus, and mink enteritis virus. Virology 129:401–414
    [Google Scholar]
  24. Parrish C. R., Carmichael L. E., Antczak D. F. 1982; Antigenic relationships between canine parvovirus type 2, feline panleukopenia virus and mink enteritis virus using conventional antisera and monoclonal antibodies. Archives of Virology 72:267–278
    [Google Scholar]
  25. Parrish C. R., O’Connell P. H., Evermann J. F., Carmichael L. E. 1985; Natural variation of canine parvovirus. Science 230:1046–1048
    [Google Scholar]
  26. Pollock R. V. H., Carmichael L. E. 1982; Dog response to inactivated canine parvovirus and feline panleukopenia virus vaccines. Cornell Veterinarian 72:16–35
    [Google Scholar]
  27. Pollock R. V. H., Carmichael L. E. 1983; Use of modified live feline panleukopenia virus vaccine to immunize dogs against canine parvovirus. American Journal of Veterinary Research 44:169–175
    [Google Scholar]
  28. Reed A. P., Jones E. V., Miller T. J. 1988; Nucleotide sequence and genome organization of canine parvovirus. Journal of Virology 62:266–276
    [Google Scholar]
  29. Rhode S. L. 1985; Nucleotide sequence of the coat protein gene of canine parvovirus. Journal of Virology 54:630–633
    [Google Scholar]
  30. Roosien J., Belsham G. J., Ryan M. D., King A. M. Q., Vlak J. M. 1990; Synthesis of foot-and-mouth disease virus capsid proteins in insect cells using baculovirus expression vectors. Journal of General Virology 71:1703–1711
    [Google Scholar]
  31. Smuda J. W., Carter B. J. 1991; Adeno-associated viruses having nonsense mutations in the capsid genes: growth in mammalian cells containing an inducible amber suppressor. Virology 184:310–318
    [Google Scholar]
  32. Summers M. D., Smith G. E. 1978; Baculovirus structural polypeptides. Virology 84:390–402
    [Google Scholar]
  33. Summers M. D., Smith G. E. 1987; A manual of methods for baculovirus vectors and insect cell culture procedures. Texas Agricultural Experiment Station Bulletin 1555
    [Google Scholar]
  34. Tattersall P., Cawte P. J., Shatkin A. J., Ward D. C. 1976; Three structural polypeptides coded for by minute virus of mice, a parvovirus. Journal of Virology 20:273–289
    [Google Scholar]
  35. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U.S.A. 76:4350–4354
    [Google Scholar]
  36. Tratschin J. D., Miller I.-L., Carter B. J. 1984; Genetic analysis of adeno-associated virus: properties of deletion mutants constructed in vitro and evidence for an adeno-associated virus replication function. Journal of Virology 51:611–619
    [Google Scholar]
  37. Tsao J., Chapman M. S., Agbandje M., Keller W., Smith K., Wu H., Luo M., Smith T. J., Rossmann M. G., Compans R. W., Parrish C. R. 1991; The three-dimensional structure of canine parvovirus and its functional implications. Science 251:1456–1464
    [Google Scholar]
  38. Vialard J., Lalumiere M., Vernet T., Briedis D., Alkhatib G., Henning D., Levin D., Richardson C. 1990; Synthesis of the membrane fusion and hemagglutinin proteins of measles virus, using a novel baculovirus vector containing the galactosidase gene. Journal of Virology 64:37–50
    [Google Scholar]
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