1887

Abstract

The myxoma virus thymidine kinase (TK) gene is encoded on a 1.6 kb I-I restriction fragment located between 57.7 and 59.3 kb on the 163 kb genomic map. The nucleotide sequence of this fragment as well as 228 bp from the adjacent I-AA2 fragment was determined and found to encode four major open reading frames (ORFs). Three of these ORFs are similar in nucleotide sequence to ORFs L5R and J1R, and the TK gene of vaccinia virus (VV). The fourth ORF, MF8a, shows similarity to the ORFs found in the same position relative to the TK genes of Shope fibroma virus, Kenya sheep-1 virus and swine-pox virus. A search of the complete VV nucleotide sequence for regions of similarity to MF8a identified the host specificity gene C7L. Northern blot analysis of early viral RNA identified transcripts of approximately 700 nucleotides for both the TK gene and ORF MF8a. The 5′ ends of the TK gene and ORF MF8a early mRNAs were mapped by primer extension to initiation sites 13 nucleotides downstream of sequences with similarity to the VV early promoter consensus. The sizes of the TK and MF8a mRNAs are consistent with transcription termination and polyadenylation occurring downstream of the sequence TTTTTNT, which is identical to the consensus sequence for the VV transcription termination signal.

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1992-02-01
2021-08-06
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References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. 1987 Current Protocols in Molecular Biology New York: John Wiley & Sons;
    [Google Scholar]
  2. Bajszar G., Wittek R., Weir J. P., Moss B. 1983; Vaccinia virus thymidine kinase and neighbouring genes: mRNAs and polypeptides of wild-type virus and putative nonsense mutants. Journal of Virology 45:62–72
    [Google Scholar]
  3. Binns M. M., Tomley F. M., Campbell J., Boursnell M. E. 1988; Comparison of a conserved region in fowlpox virus and vaccinia virus genomes and the translocation of the fowlpox virus thymidine kinase gene. Journal of General Virology 69:1275–1283
    [Google Scholar]
  4. Block W., Upton C., McFadden G. 1985; Tumorigenic poxviruses: genomic organization of malignant rabbit virus a recombinant between Shope fibroma virus and myxoma virus. Virology 140:113–124
    [Google Scholar]
  5. Boone R. F., Moss B. 1977; Methylated 5′-terminal sequences of vaccinia virus mRNA species made in vivo at early and late times after infection. Virology 79:67–80
    [Google Scholar]
  6. Boyle D. B., Coupar B. E. H. 1986; Identification and cloning of the fowlpox virus thymidine kinase gene using vaccinia virus. Journal of General Virology 67:1591–1600
    [Google Scholar]
  7. Boyle D. B., Coupar B. E. H., Gibbs A. J., Seigman L. J., Both G. W. 1987; Fowlpox virus thymidine kinase: nucleotide sequence and relationship to other thymidine kinases. Virology 156:355–365
    [Google Scholar]
  8. Chen E. Y., Seeburg P. H. 1985; Supercoil sequencing: a fast and simple method for sequencing plasmid DNA. DNA 4:165–170
    [Google Scholar]
  9. Cooper J. A., Wittek R., Moss B. 1981; Extension of the transcriptional and translational map of the left end of the vaccinia virus genome to 21 kilobase pairs. Journal of Virology 39:733–745
    [Google Scholar]
  10. Davison A. J., Moss B. 1989a; Structure of vaccinia virus early promoters. Journal of Molecular Biology 210:749–769
    [Google Scholar]
  11. Davison A. J., Moss B. 1989b; Structure of vaccinia virus late promoters. Journal of Molecular Biology 210:771–784
    [Google Scholar]
  12. Drillien R., Spehner D., Villeval D., Lecocq J.-P. 1987; Similar genetic organization between a region of fowlpox virus DNA and the vaccinia virus HindIII J fragment despite divergent location of the thymidine kinase gene. Virology 160:203–209
    [Google Scholar]
  13. Fenner F. 1976; Classification and nomenclature of viruses. Second Report of the International Committee on Taxonomy of Viruses. Intervirology 7:1–116
    [Google Scholar]
  14. Gershon P. D., Black D. N. 1989a; The nucleotide sequence around the capripoxvirus thymidine kinase gene reveals a gene shared specifically with leporipoxvirus. Journal of General Virology 70:525–533
    [Google Scholar]
  15. Gershon P. D., Black D. N. 1989b; A capripoxvirus pseudogene whose only intact homologs are in other poxvirus genomes. Virology 172:350–354
    [Google Scholar]
  16. Goebel S. J., Johnson G. P., Perkus M. E., Davis S. W., Winslow J. P., Paoletti E. 1990; The complete DNA sequence of vaccinia virus. Virology 179:247–266
    [Google Scholar]
  17. Hänggi M., Bannwarth W. E., Stunnenberg H. G. 1986; Conserved TAAAT motif in vaccinia virus late promoters: overlapping TATA box and site of transcription initiation. EMBO Journal 5:1071–1076
    [Google Scholar]
  18. Hruby D. E., Maki R. A., Miller D. B., Ball L. A. 1983; Fine structure analysis and nucleotide sequence of the vaccinia virus thymidine kinase gene. Proceedings of the National Academy of Sciences, U.S.A 80:3411–3415
    [Google Scholar]
  19. Jackson R. J., Bults H. G. 1990; A myxoma virus nucleotide sequence with homology to the vaccinia virus RNA polymerase 22-kDa subunit gene. Nucleic Acids Research 18:5290
    [Google Scholar]
  20. Keith J. M., Gershowitz A., Moss B. 1980; Dinucleotide sequence at the 5′ ends of vaccinia virus mRNAs synthesized in vitro. Journal of Virology 36:601–605
    [Google Scholar]
  21. Mahr A., Roberts B. E. 1984a; Organization of six early transcripts synthesized from a vaccinia virus Eco RI DNA fragment. Journal of Virology 49:497–509
    [Google Scholar]
  22. Mahr A., Roberts B. E. 1984b; Arrangement of late RNAs transcribed from a 71-kilobase EcoRI vaccinia virus DNA fragment. Journal of Virology 49:510–520
    [Google Scholar]
  23. Mykytowycz R. 1953; An attenuated strain of the myxomatosis virus recovered from the field. Nature, London 172:448–449
    [Google Scholar]
  24. Nevins J. R., Joklik W. K. 1975; Poly(A) sequences of vaccinia virus messenger RNA: nature, mode of addition and function during translation in vitro and in vivo. Virology 63:1–14
    [Google Scholar]
  25. Perkus M. E., Goebel S. J., Davis S. W., Johnson G. P., Limbach K., Norton E. K., Paoletti E. 1990; Vaccinia virus host range genes. Virology 179:276–286
    [Google Scholar]
  26. Piccini A., Paoletti E. 1988; Vaccinia: virus, vector, vaccine. Advances in Virus Research 34:43–64
    [Google Scholar]
  27. Plucienniczak A., Schroeder E., Zettlmeissl G., Streeck R. E. 1985; Nucleotide sequence of a cluster of early and late genes in a conserved segment of the vaccinia virus genome. Nucleic Acids Research 13:985–998
    [Google Scholar]
  28. Rohrmann G., Yuen L., Moss B. 1986; Transcription of vaccinia virus early genes by enzymes isolated from vaccinia virions terminates downstream of a regulatory sequence. Cell 46:1029–1035
    [Google Scholar]
  29. Rosel J. L., Earl P. L., Weir J. P., Moss B. 1986; Conserved TAAATG sequence at the transcriptional and translational initiation sites of vaccinia virus late genes deduced by structural and functional analysis of the Hin dIII H genome fragment. Journal of Virology 60:436–449
    [Google Scholar]
  30. Russell R. J., Robbins S. J. 1989; Cloning and molecular characterization of the myxoma virus genome. Virology 170:147–159
    [Google Scholar]
  31. Schnitzlein W. M., Tripathy D. N. 1991; Identification and nucleotide sequence of the thymidine kinase gene of swinepox virus. Virology 181:727–732
    [Google Scholar]
  32. Smith G. L., de Carlos A., Chan Y. S. 1989; Vaccinia virus encodes a thymidylate kinase gene: sequence and transcriptional mapping. Nucleic Acids Research 17:7581–7590
    [Google Scholar]
  33. Upton C., McFadden G. 1986; Identification and nucleotide sequence of the thymidine kinase gene of Shope fibroma virus. Journal of Virology 60:920–927
    [Google Scholar]
  34. Upton C., DeLange A. M., McFadden G. 1987a; Tumorigenic poxvirus: genomic organization and DNA sequence of the telomeric region of the Shope fibroma virus genome. Virology 160:20–30
    [Google Scholar]
  35. Upton C., Macen J. L., McFadden G. 1987b; Mapping and sequencing of a gene from myxoma virus that is related to those encoding epidermal growth factor and transforming growth factor alpha. Journal of Virology 61:1271–1275
    [Google Scholar]
  36. Upton C., Macen J. L., Wishart D. S., McFadden G. 1990; Myxoma virus and malignant rabbit fibroma virus encode a serpin-like protein important for virus virulence. Virology 179:618–631
    [Google Scholar]
  37. Yuen L., Moss B. 1986; Multiple 3′ ends of mRNA encoding vaccinia virus growth factor occur within a series of repeated sequences downstream of T clusters. Journal of Virology 60:320–323
    [Google Scholar]
  38. Yuen L., Moss B. 1987; Oligonucleotide sequence signalling transcriptional termination of vaccinia virus early genes. Proceedings of the National Academy of Sciences, U.S.A 84:6417–6421
    [Google Scholar]
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