1887

Abstract

Recent studies have shown that infection with herpes simplex virus type 1 (HSV-1) strain 17 generates in addition to virions a novel type of non-infectious particle. These particles, termed L particles, lack capsids and viral DNA, and consist predominantly of tegument and envelope proteins. We show that L particle production is not restricted to one strain of HSV-1, and that pseudorabies virus and equine herpesvirus type 1 also release particles which are similar in composition to and morphologically indistinguishable from HSV-1 L particles. Data obtained from monoclonal antibody analysis revealed that Vmw175, an immediate early HSV-1 polypeptide which had been previously identified as a virion component, is located predominantly in L particles and not in virions. Following removal of the envelope from L particles, the remaining tegument material largely retained its structural integrity, indicating that the structure of the tegument does not depend on the presence of the capsid or envelope.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-2-269
1992-02-01
2022-11-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/2/JV0730020269.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-2-269&mimeType=html&fmt=ahah

References

  1. Bibor-Hardy V., Sakr F. 1989; A 165kd protein of the herpes simplex virus virion shares a common epitope with the regulatory protein, ICP4. Biochemical and Biophysical Research Communications 163:124–130
    [Google Scholar]
  2. Braun D. K., Roizman B., Pereira L. 1984; Characterization of post-translational products of herpes simplex virus gene 35 proteins binding to the surfaces of full capsids but not empty capsids. Journal of Virology 49:142–153
    [Google Scholar]
  3. Brown S. M., Ritchie D. A., Subak-Sharpe J. H. 1973; Genetic studies with herpes simplex virus type 1. The isolation of temperature-sensitive mutants, their arrangement into complementation groups and recombination analysis leading to a linkage map. Journal of General Virology 18:329–346
    [Google Scholar]
  4. Caughman G. B., Staczek J., O’Callaghan D. J. 1985; Equine herpesvirus type 1 infected cell polypeptides: evidence for immediate early/early/late regulation of viral gene expression. Virology 145:49–61
    [Google Scholar]
  5. Caughman G. B., Robertson A. T., Gray W. I., Sullivan D. C., O’Callaghan D. J. 1988; Characterization of equine herpesvirus type 1 immediate early proteins. Virology 163:563–571
    [Google Scholar]
  6. Dargan D. J. 1986; The structure and assembly of herpesviruses. In Electron Microscopy of Proteins vol 5Virus Structure pp 359–437 Edited by Harris J. R., Horne R. W. London: Academic Press;
    [Google Scholar]
  7. Ejercito P. M., Kieff E. D., Roizman B. 1968; Characterization of herpes simplex virus strains differing in their effects on social behaviour of infected cells. Journal of General Virology 2:357–364
    [Google Scholar]
  8. Fenwick M. L., Everett R. D. 1990; Inactivation of the shutoff gene (UL41) of herpes simplex virus types 1 and 2. Journal of General Virology 71:2961–2967
    [Google Scholar]
  9. Grundy F. J., Baumann R. P., O’Callaghan D. J. 1989; DNA sequence and comparative analyses of the equine herpesvirus type 1 immediate early gene. Virology 172:223–236
    [Google Scholar]
  10. Heine J. W., Honess R. W., Cassai E., Roizman B. 1974; Proteins specified by herpes simplex virus. XII. The virion polypeptides of type 1 strains. Journal of Virology 14:640–651
    [Google Scholar]
  11. Ihara S., Feldman L., Watanabe S., Ben-Porat T. 1983; Characterization of the immediate-early functions of pseudorabies virus. Virology 131:437–454
    [Google Scholar]
  12. Irmiere A., Gibson W. 1983; Isolation and characterization of a noninfectious virion-like particle released from cells infected with human strains of cytomegalovirus. Virology 130:118–133
    [Google Scholar]
  13. Kaplan A. S., Vatter A. E. 1959; A comparison of herpes simplex and pseudorabies viruses. Virology 7:394–407
    [Google Scholar]
  14. Killington R. A., Yeo J., Honess R. W., Watson D. H., Duncan B. E., Halliburton I. W., Mumford J. 1977; Comparative analyses of the proteins and antigens of five herpesviruses. Journal of General Virology 37:297–310
    [Google Scholar]
  15. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  16. Longnecker R., Roizman B. 1986; Generation of an inverting herpes simplex 1 mutant lacking the L-S junction ‘a’ sequences, an origin of DNA synthesis, and several genes including those specifying glycoprotein E and the 47 gene. Journal of Virology 58:583–591
    [Google Scholar]
  17. McCombs R. M., Brunschwig J. P., Mirkovic R., Benyesh-Melnick M. 1971; Electron microscopic characterization of a herpeslike virus isolated from tree shrews. Virology 45:816–820
    [Google Scholar]
  18. McLean C. A., Clark B., McGeoch D. J. 1989; Gene ULI 1 of herpes simplex virus type 1 encodes a virion protein which is myristylated. Journal of General Virology 70:3147–3157
    [Google Scholar]
  19. McLean G., Rixon F., Langeland N., Haarr L., Marsden H. 1990; Identification and characterization of the virion protein products of herpes simplex virus type 1 gene UL47. Journal of General Virology 71:2953–2960
    [Google Scholar]
  20. Marsden H. S., Crombie I. K., Subak-Sharpe J. H. 1976; Control of protein synthesis in herpesvirus-infected cells: analysis of the polypeptides induced by wild type and sixteen temperaturesensitive mutants of HSV strain 17. Journal of General Virology 31:347–372
    [Google Scholar]
  21. Misra V., Blumenthal R. M., Babiuk L. A. 1981; Proteins specified by bovine herpesvirus 1 (infectious bovine rhinotracheitis virus). Journal of Virology 40:367–378
    [Google Scholar]
  22. Nazerian K., Witter R. L. 1970; Cell-free transmission and in vivo replication of Marek’s disease virus. Journal of Virology 5:388–397
    [Google Scholar]
  23. Read G. S., Frenkel N. 1983; Herpes simplex virus mutants defective in the virion-associated shutoff of host polypeptide synthesis and exhibiting abnormal synthesis of alpha (immediate-early) polypeptides. Journal of Virology 46:498–512
    [Google Scholar]
  24. Rixon F. J., Davison M. D., Davison A. J. 1990; Identification of the genes encoding two capsid proteins of herpes simplex virus type 1 by direct amino acid sequencing. Journal of General Virology 71:1211–1214
    [Google Scholar]
  25. Rixon F. J., Addison C., McLauchlan J. 1992; Assembly of enveloped tegument structures (L particles) can occur independently of virion maturation in herpes simplex virus type 1-infected cells. Journal of General Virology 73:277–284
    [Google Scholar]
  26. Robertson A. T., Caughman G. B., Gray W. L., Baumann R. P., Staczek J., O’Callaghan D. J. 1988; Analysis of the in vitro translation products of the equine herpesvirus type 1 immediate early mRNA. Virology 166:451–462
    [Google Scholar]
  27. Roizman B., Furlong D. 1974; The replication of herpesviruses. Comprehensive Virology 3:229–403
    [Google Scholar]
  28. Schrag J. D., Venkataram Prasad B. V., Rixon F. J., Chiu W. 1989; Three-dimensional structure of the HSV-1 nucleocapsid. Cell 56:651–660
    [Google Scholar]
  29. Showalter S. D., Zweig M., Hampar B. 1981; Monoclonal antibodies to herpes simplex virus type 1 proteins, including the immediate-early protein ICP4. Infection and Immunity 34:684–692
    [Google Scholar]
  30. Spear P. G., Roizman B. 1972; Proteins specified by herpes simplex virus. V. Purification and structural proteins of the herpesvirion. Journal of Virology 9:143–159
    [Google Scholar]
  31. Szilagyi J. F., Cunningham C. 1991; Identification and characterization of a novel non-infectious herpes simplex virus-related particle. Journal of General Virology 72:661–668
    [Google Scholar]
  32. Talbot P., Almeida J. D. 1977; Human cytomegalovirus: purification of enveloped virions and dense bodies. Journal of General Virology 36:345–349
    [Google Scholar]
  33. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U.S.A. 76:4350–4354
    [Google Scholar]
  34. Umene K. 1986; Conversion of a fraction of the unique sequence to part of the inverted repeats in the S component of the herpes simplex virus type 1 genome. Journal of General Virology 67:1035–1048
    [Google Scholar]
  35. Vernon S. K., Lawrence W. C., Long C. A., Rubin B. A., Sheffield J. B. 1982; Morphological components of herpesvirus. IV. Ultrastructural features of the envelope and tegument. Journal of Ultrastructure Research 81:163–171
    [Google Scholar]
  36. Vlcek C., Paces V., Schwyver M. 1989; Nucleotide sequence of the pseudorabies virus immediate early gene, encoding a strong trans-activator protein. Virus Genes 2:335–346
    [Google Scholar]
  37. Wildy P., Russell W. C., Horne R. W. 1960; The morphology of herpes virus. Virology 12:204–222
    [Google Scholar]
  38. Yao F., Courtney R. J. 1989; A major transcriptional regulatory protein (ICP4) of herpes simplex virus type 1 is associated with purified virions. Journal of Virology 63:3338–3344
    [Google Scholar]
  39. Yao F., Courtney R. J. 1991; Association of a major transcriptional regulatory protein, ICP4, of herpes simplex virus type 1 with the plasma membrane of virus-infected cells. Journal of Virology 65:1516–1524
    [Google Scholar]
  40. Zhang Y., Sirko D. A., McKnight J. L. C. 1991; Role of herpes simplex virus type 1 UL46 and UL47 in αTIF-mediated transcriptional induction: characterization of three viral deletion mutants. Journal of Virology 65:829–841
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-2-269
Loading
/content/journal/jgv/10.1099/0022-1317-73-2-269
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error