1887

Abstract

In addition to ss and ds genomic DNA, agroinoculation of plants with the Logan strain of the geminivirus beet curly top virus (BCTV) consistently resulted in production of subgenomic DNAs on initial passage. Single-stranded and dsDNA forms representing at least seven size classes (0.8 to 1.8 kb) of subgenomic DNA were observed in total DNA extracts from inoculated plants. Extracts from infected sugar beet and tomato contained variable but usually smaller amounts of subgenomic DNAs, suggesting that their production may be influenced by the host species. Restriction endonuclease mapping and partial nucleotide sequencing of three independent clones of a 1.5 kb size class indicated that this subgenomic DNA is produced from the standard viral genome by two separate deletion events. One deletion of 941 bp includes portions of the leftward open reading frames (ORFs) L1, L2 and L3, while the other deletion of 579 bp encompasses portions of the intergenic region and the rightward ORFs R1, R2 and R3. The data indicate that the 1.5 kb BCTV subgenomic DNA is a defective DNA that has retained ciselements essential for replication.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-2-237
1992-02-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/2/JV0730020237.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-2-237&mimeType=html&fmt=ahah

References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Siedman J. G., Smith J. A., Struhl K. 1987 Current Protocols in Molecular Biology New York: John Wiley and Sons;
    [Google Scholar]
  2. Bisaro D. M., Sunter G., Revington G. N., Brough C. L., Hormuzdi S. G., Hartitz M. D. 1990; Molecular genetics of tomato golden mosaic virus replication: progress towards defining gene functions, transcription units and the origin of DNA replication. In Viral Genes and Plant Pathogenesis pp 89–105 Edited by Pirone T. P., Shaw J. G. New York: Springer-Verlag;
    [Google Scholar]
  3. Briddon R. W., Watts J., Markham P. G., Stanley J. 1989; The coat protein of beet curly top virus is essential for infectivity. Virology 172:628–633
    [Google Scholar]
  4. Czosnek H., Ber R., Navot N., Antignus Y., Cohen S., Zamir D. 1989; Tomato yellow leaf curl virus DNA forms in the viral capsid, in infected plants and in the insect vector. Journal of Phytopathology 125:47–54
    [Google Scholar]
  5. Davies J. W., Stanley J. 1989; Geminivirus genes and vectors. Trends in Genetics 5:77–81
    [Google Scholar]
  6. Frischmuth T., Stanley J. 1991; African cassava mosaic virus DI DNA interferes with the replication of both genomic components. Virology 183:539–544
    [Google Scholar]
  7. Grimsley N., Hohn T., Davies J. W., Hohn B. 1987; Agrobacterium-mediated delivery of infectious maize streak virus into maize plants. Nature, London 325:177–179
    [Google Scholar]
  8. Hamilton W. D. O., Bisaro D. M., Buck K. W. 1982; Identification of novel DNA forms in tomato golden mosaic virus infected tissue. Evidence for a two component viral genome. Nucleic Acids Research 10:4901–4912
    [Google Scholar]
  9. Hamilton W. D. O., Bisaro D. M., Coutts R. H. A., Buck K. W. 1983; Demonstration of the bipartite nature of the genome of a single-stranded DNA plant virus by infection with the cloned DNA components. Nucleic Acids Research 11:7387–7396
    [Google Scholar]
  10. Hamilton W. D. O., Stein V. E., Coutts R. H. A., Buck K. W. 1984; Complete nucleotide sequence of the infectious cloned DNA components of tomato golden mosaic virus: potential coding regions and regulatory sequences. EMBO Journal 3:2197–2205
    [Google Scholar]
  11. Hayes R. J., Brough C. L., Prince V. E., Coutts R. H. A., Buck K. W. 1988; Infection of Nicotiana benthamiana with uncut cloned tandem dimers of tomato golden mosaic virus DNA. Journal of General Virology 69:209–218
    [Google Scholar]
  12. Holland J. J. 1990; Defective viral genomes. In Virology pp 151–165 Edited by Fields B. N., Knipe D. M. New York: Raven Press;
    [Google Scholar]
  13. Lazarowitz S. G. 1987; The molecular characterization of geminiviruses. Plant Molecular Biology Reporter 4:177–192
    [Google Scholar]
  14. Macdonald H., Coutts R. H. A., Buck K. W. 1988; Characterization of a subgenomic DNA isolated from Triticum aestivum plants infected with wheat dwarf virus. Journal of General Virology 69:1339–1344
    [Google Scholar]
  15. MacDowell S. W., Macdonald H., Hamilton W. D. O., Coutts R. H. A., Buck K. W. 1985; The nucleotide sequence of cloned wheat dwarf virus DNA. EMBO Journal 4:2173–2180
    [Google Scholar]
  16. MacDowell S. W., Coutts R. H. A., Buck K. W. 1986; Molecular Characterisation of subgenomic single-stranded and double-stranded DNA forms isolated from plants infected with tomato golden mosaic virus. Nucleic Acids Research 14:7967–7984
    [Google Scholar]
  17. Messing J., Vieira J. 1982; A new pairof M13 vectorsforselecting either DNA Strand of double-digest restriction fragments. Gene 19:269–276
    [Google Scholar]
  18. Revington G. N., Sunter G., Bisaro D. M. 1989; DNA sequences essential for replication of the B genome component of tomato golden mosaic virus. Plant Cell 1:985–992
    [Google Scholar]
  19. Rochester D. E., Kositratana W., Beachy R. N. 1990; Systemic movement and Symptom production following agroinoculation with a single DNA of tomato yellow leaf curl geminivirus (Thailand). Virology 178:520–526
    [Google Scholar]
  20. Rogers S. G., Bisaro D. M., Horsch R. B., Fraley R. T., Hoffman N. L., Brand L., Elmer J. S., Lloyd A. M. 1986; Tomato golden mosaic virus A component DNA replicates autonomously in transgenic plants. Cell 45:593–600
    [Google Scholar]
  21. Rogers S. G., Klee H. J., Horsch R. B., Fraley R. T. 1987; Improved vectors for plant transformation: expression cassette vectors and new selectable markers. Methods in Enzymology 153:253–277
    [Google Scholar]
  22. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  23. Saunders K., Lucy A., Stanley J. 1991; DNA forms of the geminivirus African cassava mosaic virus consistent with a rolling circle mechanism of replication. Nucleic Acids Research 19:2325–2330
    [Google Scholar]
  24. Stanley I. 1983; Infectivity of cloned geminivirus genome requires sequences from both DNAs. Nature, London 305:643–645
    [Google Scholar]
  25. Stanley J., Gay M. R. 1983; Nucleotide sequence of cassava latent virus DNA. Nature, London 301:260–262
    [Google Scholar]
  26. Stanley J., Townsend R. 1985; Characterization of DNA forms associated with cassava latent virus infection. Nucleic Acids Research 13:2189–2206
    [Google Scholar]
  27. Stanley I., Markham P. G., Callis R. J., Pinner M. S. 1986; The nucleotide sequence of an infectious clone of the geminivirus beet curly top virus. EMBO Journal 5:1761–1767
    [Google Scholar]
  28. Stanley J., Frischmuth T., Ellwood S. 1990; Defective viral DNA ameliorates Symptoms of geminivirus infection in transgenic plants. Proceedings of the National Academy of Sciences, U.S.A 87:6291–6295
    [Google Scholar]
  29. Stenger D. C., Carbonaro D., Duffus J. E. 1990; Genomic characterization of phenotypic variants of beet curly top virus. Journal of General Virology 71:2211–2215
    [Google Scholar]
  30. Stenger D. C., Revington G. N., Stevenson M. C., Bisaro D. M. 1991; Replicational release of geminivirus genomes from tandemly repeated copies: evidence for rolling circle replication of a plant viral DNA. Proceedings of the National Academy of Sciences, U,. S,. A (in press)
    [Google Scholar]
  31. Sunter G., Gardiner W. E., Rushing A. E., Rogers S. G., Bisaro D. M. 1987; Independent encapsidation of tomato golden mosaic virus A component DNA in transgenic plants. Plant Molecular Biology 8:477–484
    [Google Scholar]
  32. Townsend R., Watts I., Stanley J. 1986; Synthesis of viral DNA forms in Nicotiana plumbaginafolia protoplasts inoculated with cassava latent virus (CLV); evidence for independent replication of one component of the CLV genome. Nucleic Acids Research 10:6849–6863
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-2-237
Loading
/content/journal/jgv/10.1099/0022-1317-73-2-237
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error