1887

Abstract

The inability to culture human parvovirus B19 in standard cell lines has rendered investigation of clinical samples for the presence of infectious virus problematic. Using haematopoietic precursors derived from first trimester foetal liver as targets for infection, and non-isotopic hybridization to detect intracellular virual DNA, we have assessed infectivity in stored serum samples taken from nine volunteers at different stages following intranasal inoculation with parvovirus B19. Infectious virus was detected as early as 3 days after inoculation, the cessation of infectivity correlating with the rise in specific IgM. In all but two samples, infectivity correlated with the detection of B19 DNA by dot-blot hybridization, although culture was 10-fold more sensitive than dot-blot hybridization. B19 DNA was detected by the polymerase chain reaction in serum from one volunteer up to 36 days after inoculation, although samples containing specific antibody were non-infectious. Infection of erythroid precursors was completely inhibited by preincubation of virus with serum containing high titre B19-specific IgM and IgG. Unexpectedly, this was associated with a strong B19 DNA hybridization signal within the cytoplasm of phagocytic macrophages. This culture and detection system is a rapid and sensitive means of detecting infectious virus in serum samples, and of assessing the neutralizing ability of B19-specific antibodies.

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/content/journal/jgv/10.1099/0022-1317-73-12-3313
1992-12-01
2022-09-25
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References

  1. Anderson M. J., Lewis E., Kidd I. M., Cohen B. J. 1984; An outbreak of erythema infectiosum associated with human parvovirus infection. Journal of Hygiene, London 93:85–93
    [Google Scholar]
  2. Anderson M. J., Jones S. E., Minson A. C. 1985a; Diagnosis of human parvovirus infection by dot-blot hybridization using cloned viral DNA. Journal of Medical Virology 15:163–172
    [Google Scholar]
  3. Anderson M. J., Higgins P. G., Davis L. R., Willman J. S., Jones S. E., Kidd I. M., Pattison J. R., Tyrrell D. A. J. 1985b; Experimental parvovirus infection in humans. Journal of Infectious Diseases 152:257–265
    [Google Scholar]
  4. Brown K. E., Mori J., Cohen B. J., Field A. M. 1991; In vitro propagation of parvovirus B19 in primary foetal liver culture. Journal of General Virology 72:741–745
    [Google Scholar]
  5. Brown T., Anand A., Ritchie L. D., Clewley J. P., Reid T. M. S. 1984; Intrauterine human parvovirus infection and hydrops fetalis. Lancet ii:1033–1034
    [Google Scholar]
  6. Cohen B. J., Mortimer P. P., Pereira M. S. 1983; Diagnostic assays with monoclonal antibodies for the human serum parvovirus-like virus (SPLV). Journal of Hygiene, Cambridge 91:113–130
    [Google Scholar]
  7. Hanada T., Koike K., Takeya T., Nagasawa T., Matsunaga Y., Takita H. 1988; Human parvovirus B19-induced transient pancytopenia in a child with hereditary spherocytosis. British Journal of Haematology 70:113–115
    [Google Scholar]
  8. Kurtzman G. J., Ozawa K., Cohen B., Hanson G., Oseas R., Young N. S. 1987; Chronic bone marrow failure due to persistent B19 parvovirus infection. New England Journal of Medicine 317:287–294
    [Google Scholar]
  9. Kurtzman G. J., Gascon P., Caras M., Cohen B., Young N. 1988; B19 parvovirus replicates in circulating cells of acutely infected patients. Blood 71:1448–1454
    [Google Scholar]
  10. Kurtzman G. J., Cohen B. J., Field A. M., Oseas R., Blaese R. M., Young N. S. 1989; Immune response to B19 parvovirus and an antibody defect in persistent viral infection. Journal of Clinical Investigation 84:1114–1123
    [Google Scholar]
  11. Morey A. L., Fleming K. A. 1992; Immunophenotyping of fetal haemopoietic cells permissive for human parvovirus B19 replication in vitro . British Journal of Haematology 82: (in press)
    [Google Scholar]
  12. Morey A. L., Nicolini U., Welch C. R., Chamberlain P. F., Economides D., Cohen B. J. 1991; Parvovirus B19 infection and transient fetal hydrops. Lancet 337:496
    [Google Scholar]
  13. Mortimer P. P., Humphries R. K., Moore J. G., Purcell R. H., Young N. S. 1983; A human parvovirus-like virus inhibits haematopoietic colony formation in vitro . Nature, London 302:426–429
    [Google Scholar]
  14. Ozawa K., Kurtzman G., Young N. 1986; Replication of the B19 parvovirus in human bone marrow cell cultures. Science 233:883–886
    [Google Scholar]
  15. Ozawa K., Kurtzman G., Young N. 1987; Productive infection by B19 parvovirus of human erythroid bone marrow cells in vitro . Blood 70:384–391
    [Google Scholar]
  16. Patou G., Ayliffe U. 1991; Evaluation of commercial enzyme linked immunosorbent assay for detection of B19 parvovirus IgM and IgG. Journal of Clinical Pathology 44:851–854
    [Google Scholar]
  17. Pattison J. R., Jones S. E., Hodgson J., Davies L. R., White J. M., Stroud C. E., Murtaza L. 1981; Parvovirus infections and hypoplastic crises in sickle cell anaemia. Lancet i:664–665
    [Google Scholar]
  18. Potter C. G., Potter A. C., Hatton C. S. R., Chapel H. M., Anderson M. J., Pattison J. R., Tyrrell D. A. J., Higgins P. G., Willman J. S., Parry H. F., Cotes P. M. 1987; Variation of erythroid and myeloid precursors in the marrow and peripheral blood of volunteer subjects infected with human parvovirus (B19). Journal of Clinical Investigation 79:1486–1492
    [Google Scholar]
  19. Saarinen U. M., Chorba T. L., Tattersall P., Young N. S., Anderson L. J., Palmer E., Coccia P. F. 1986; Human parvovirus B19-induced epidemic acute red cell aplasia in patients with hereditary hemolytic anemia. Blood 67:1411–1417
    [Google Scholar]
  20. Serke S., Schwarz T. F., Baurmann H., Kirsch A., Hottentrager B., von Brunn A., Roggendorf M., Huhn D., Deinhardt F. 1991; Productive infection of in vitro generated haemopoietic progenitor cells from normal human adult peripheral blood with parvovirus B19: studies by morphology, immunocyto-chemistry, flow-cytometry and DNA-hybridization. British Journal of Haematology 79:6–13
    [Google Scholar]
  21. Srivastava A., Lu L. 1988; Replication of B19 parvovirus in highly enriched hematopoietic progenitor cells from normal human bone marrow. Journal of Virology 62:3059–3063
    [Google Scholar]
  22. Yaegashi N., Shiraishi H., Takeshita T., Nakamura M., Yajima A., Sugamura K. 1989; Propagation of human parvovirus B19 in primary culture of erythroid lineage cells derived from fetal liver. Journal of Virology 63:2422–2426
    [Google Scholar]
  23. Young N. S., Mortimer P. P., Moore J. G., Humphries R. K. 1984; Characterisation of a virus that causes transient aplastic crisis. Journal of Clinical Investigation 73224–230
    [Google Scholar]
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