1887

Abstract

Primary cultures of normal human keratinocytes were inoculated with human papillomavirus type 1 (HPV-1), the agent responsible for deep plantar warts. Upon transfer to dead de-epidermized dermis and growth at the air-liquid interface, keratinocytes reconstituted a pseudoepidermis. Under these highly differentiating conditions, HPV-1 DNA amplification was found to take place in the reconstructed epidermis, being detectable from 7 days after the transfer and persisting for at least 10 days thereafter. The extent of keratinocyte differentiation may be insufficient to allow a complete HPV infectious cycle.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-12-3269
1992-12-01
2022-08-11
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/12/JV0730123269.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-12-3269&mimeType=html&fmt=ahah

References

  1. Asselineau D., Bernard B. A., Bailly C., Darmon M., Prunieras M. 1986; Human epidermis reconstructed by culture: is it ‘normal’?. Journal of Investigative Dermatology 86:181–186
    [Google Scholar]
  2. Bedell M. A., Hudson J. B., Golub T. R., Turyk M. E., Hosken M., Wilbanks G. D., Laimins L. A. 1991; Amplification of human papillomavirus genomes in vitro is dependent on epithelial differentiation. Journal of Virology 65:2254–2260
    [Google Scholar]
  3. Boyce S., Ham R. 1983; Calcium-regulated differentiation of normal human epidermal keratinocytes in chemically defined clonal culture and serum-free serial culture. Journal of Investigative Dermatology 81:33s–43s
    [Google Scholar]
  4. Cook R. H., Olson C. 1951; Experimental transmission of cutaneous papilloma of the horse. American Journal of Pathologv 27:1087–1097
    [Google Scholar]
  5. Favre M., Orth G., Croissant O., Yaniv M. 1975; Human papillomavirus DNA: physical map. Proceedings of the National Academy of Sciences, U.S.A. 72:4810–4814
    [Google Scholar]
  6. Gissmann L., Pfister H., zur Hausen H. 1977; Human papillomavirus (HPV): characterization of 4 different isolates. Virology 76:563–580
    [Google Scholar]
  7. Grussendorf E. I., zur Hausen H. 1979; Localisation of viral DNA replication in sections of human warts by nucleic acid hybridization with complementary RNA of human papillomavirus type 1. Archives of Dermatological Research 264:55–63
    [Google Scholar]
  8. Jelinek W. R., Schmid C. W. 1982; Repetitive sequences in eukaryotic DNA and their expression. Annual Review of Biochemistry 51:813–844
    [Google Scholar]
  9. Kreider J. W., Patrick S. D., Cladel N. M., Welsh P. A. 1990; Experimental infection with human papillomavirus type 1 of human hand and foot skin. Virology 177:415–417
    [Google Scholar]
  10. La Porta R. F., Taichman L. B. 1982; Human papilloma viral DNA replicates as a stable episome in cultured epidermal keratinocytes. Proceedings of the National Academy of Sciences, U.S.A. 79:3393–3397
    [Google Scholar]
  11. Oriel J. D. 1971; Natural history of genital warts. British Journal of Venereal Diseases 47:1–13
    [Google Scholar]
  12. Orth G., Breitburd F., Favre M., Croissant O. 1977; Papillomaviruses: possible role in human cancer. In Cold Spring Harbor Conferences on Cell Proliferation vol 4 pp. 1043–1068 Edited by Hiatt H. H., Watson J. D., Wisten J. A. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  13. Praeger F. C., Stanulis-Praeger B. M., Gilchrist B. A. 1987; Use of strontium to separate calcium-dependent pathways for proliferation and differentiation in human keratinocytes. Journal of Cellular Physiology 132:81–89
    [Google Scholar]
  14. Prunieras M., Regnier M., Woodley P. 1983; Methods for cultivation of keratinocytes with an air-liquid interface. Journal of Investigative Dermatology 81:28s–33s
    [Google Scholar]
  15. Rubin C. M., Houck C. M., Deininger P. L., Friedmann T., Schmid C. W. 1980; Partial nucleotide sequence of the 300 nucleotide interspersed repeated human DNA sequences. Nature, London 284:372–374
    [Google Scholar]
  16. Schneider A. 1987; Methods of identification of human papillomaviruses. In Papillomaviruses and Human Disease pp. 19–39 Edited by Syrjanen K., Gissmann L., Koss L. G. Heidelberg: Springer Verlag;
    [Google Scholar]
  17. Sterling G., Stanley M., Gatward G., Minson T. 1990; Production of human papillomavirus type 16 virions in a keratin-ocyte cell line. Journal of Virology 64:6305–6307
    [Google Scholar]
  18. Taichman L. B., Breitburd F., Croissant O., Orth G. 1984; The search for a culture system for papillomavirus. Journal of Investigative Dermatology 83:2s–6s
    [Google Scholar]
  19. Tsao M. C., Walthall B. J., Ham R. G. 1982; Clonal growth of normal human epidermal keratinocytes in a defined medium. Journal of Cellular Physiology 110:219–229
    [Google Scholar]
  20. zur Hausen H. 1985; Genital papillomavirus infections. Progress in Medical Virology 32:15–21
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-12-3269
Loading
/content/journal/jgv/10.1099/0022-1317-73-12-3269
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error