%0 Journal Article %A Smits, Henk L. %A Tieben, Linda M. %A Tjong-A-Hung, Steven P. %A Jebbink, Maarten F. %A Minnaar, Rene P. %A Jansen, Casper L. %A ter Schegget, Jan %T Detection and typing of human papillomaviruses present in fixed and stained archival cervical smears by a consensus polymerase chain reaction and direct sequence analysis allow the identification of a broad spectrum of human papillomavirus types %D 1992 %J Journal of General Virology, %V 73 %N 12 %P 3263-3268 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-73-12-3263 %I Microbiology Society, %X DNA well suited for polymerase chain reaction (PCR) amplification was purified from archival Papanicolaou smears. The detection of a wide range of human papillomavirus (HPV) types was made possible using a HPV-specific consensus primer pair, and typing was conveniently done by direct sequence analysis of the PCR product. The method could be of unique value in longitudinal and cross-sectional studies aimed at answering a number of fundamental pathological and epidemiological questions regarding HPV infection of the genital tract. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-73-12-3263