RT Journal Article SR Electronic(1) A1 Pise, Cynthia A. A1 Newburger, Peter E. A1 Holland, Christie A.YR 1992 T1 Human immunodeficiency virus type 1-infected HL-60 cells are capable of both monocytic and granulocytic differentiation JF Journal of General Virology, VO 73 IS 12 SP 3257 OP 3261 DO https://doi.org/10.1099/0022-1317-73-12-3257 PB Microbiology Society, SN 1465-2099, AB We have used the human myelomonocytic cell line HL-60 as a model system to determine whether human immunodeficiency virus type 1 (HIV-1) infection affects differentiation of myeloid progenitor cells. HL-60 cells were infected with three HIV-1 isolates (IIIB, NL4-3 and PM213). HIV-1 antigen expression and cytopathicity in HL-60 cells infected with each of the three isolates was delayed by approximately 15 days as compared to those in the prototypic T cell line, H9. Chronically infected HL-60 cells and clonal lines derived from them were treated with dimethyl formamide (DMF) and induced to differentiate into granulocytes. Approximately the same percentage of these cells as of DMF-treated, uninfected HL-60 cells differentiated. Superoxide production by infected and uninfected DMF-induced cells was similar. Likewise, approximately the same percentage of cells in infected and uninfected cultures became adherent and were positive for non-specific esterase when monocytic differentiation was induced. The data demonstrate that HL-60 cells infected with HIV-1 are capable of morphological and functional granulocytic and monocytic differentiation., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-73-12-3257