1887

Journal of General Virology header logo

Volume 73, Issue 12

Human immunodeficiency virus type 1-infected HL-60 cells are capable of both monocytic and granulocytic differentiation Free

Abstract

We have used the human myelomonocytic cell line HL-60 as a model system to determine whether human immunodeficiency virus type 1 (HIV-1) infection affects differentiation of myeloid progenitor cells. HL-60 cells were infected with three HIV-1 isolates (IIIB, NL4-3 and PM213). HIV-1 antigen expression and cytopathicity in HL-60 cells infected with each of the three isolates was delayed by approximately 15 days as compared to those in the prototypic T cell line, H9. Chronically infected HL-60 cells and clonal lines derived from them were treated with dimethyl formamide (DMF) and induced to differentiate into granulocytes. Approximately the same percentage of these cells as of DMF-treated, uninfected HL-60 cells differentiated. Superoxide production by infected and uninfected DMF-induced cells was similar. Likewise, approximately the same percentage of cells in infected and uninfected cultures became adherent and were positive for non-specific esterase when monocytic differentiation was induced. The data demonstrate that HL-60 cells infected with HIV-1 are capable of morphological and functional granulocytic and monocytic differentiation.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1992
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-12-3257
1992-12-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/12/JV0730123257.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-12-3257&mimeType=html&fmt=ahah

References

  1. Baehner R. L., Nathan D. G. 1968; Quantitative nitroblue tetrazolium test in chronic granulomatous disease. New England Journal of Medicine 278:971–976
     [Google Scholar]
  2. Bender B. S., Davidson B. L., Kline R., Brown C., Quinn T. C. 1988; Role of the mononuclear phagocyte system in the immuno-pathogenesis of human immunodeficiency virus infection and the acquired immunodeficiency syndrome. Reviews of Infectious Diseases 10:1142–1154
     [Google Scholar]
  3. Butera S. T., Perez V. L., Besansky N. J., Chan W. C., Wu B., Nabel G. J., Folks T. M. 1991; Extrachromosomal human immunodeficiency virus type-1 DNA can initiate a spreading infection of HL-60 cells. Journal of Cellular Biochemistry 45:366–373
     [Google Scholar]
  4. Carlo-Stella C., Ganser A., Hoelzer D. 1987; Defective in vitro growth of the hematopoeitic progenitor cells in the acquired immunodeficiency syndrome. Journal of Clinical Investigation 80:286–293
     [Google Scholar]
  5. Clapham P. R., Weiss R. A., Dalgleish A. G., Exley M., Whitby D., Hogg N. 1987; Human immunodeficiency virus infection of monocytic and T-lymphocytic cells: receptor modulation and differentiation induced by phorbol ester. Virology 158:44–51
     [Google Scholar]
  6. Cloyd M. W., Moore B. E. 1990; Spectrum of biological properties of human immunodeficiency virus (HIV-1) isolates. Virology 174:103–116
     [Google Scholar]
  7. Collins S. 1987; The HL-60 promyelocytic leukemia cell line: proliferation, differentiation, and cellular oncogene expression. Blood 70:1233–1244
     [Google Scholar]
  8. Donahue R. E., Johnson M. M., Zon L. I., Clark S. C., Groopman J. E. 1987; Suppression of in vitro haematopoiesis following human immunodeficiency virus infection. Nature, London 326:200–203
     [Google Scholar]
  9. Ennen J., Seipp I., Norley S. G., Kurth R. 1990; Decreased accessory cell function of macrophages after infection with human immunodeficiency virus type 1 in vitro . European Journal of Immunology 20:2451–2456
     [Google Scholar]
  10. Folks T. M., Kessler S. W., Orenstein J. M., Justement J. S., Jaffe E. S., Fauci A. S. 1988; Infection and replication of HIV-1 in purified progenitor cells of normal human bone marrow. Science 242:919–922
     [Google Scholar]
  11. Gendelman H. E., Orenstein J. M., Martin M. A. 1986; Efficient isolation and propagation of human immunodeficiency virus on recombinant colony stimulating factor 1-treated monocytes. Journal of Experimental Medicine 167:1428–1441
     [Google Scholar]
  12. Gendelman H. E., Orenstein J. M., Baca L. M., Weiser B., Burger H., Kalter D. C., Meltzer M. S. 1989; The macrophage in the persistence and pathogenesis of HIV infection. AIDS 3:475–495
     [Google Scholar]
  13. Groopman J. E., Mitsuyasu R. T., DeLeo M. J., Oette D. H., Golde D. W. 1987; Effect of recombinant human granulocyte-macrophage colony-stimulating factor on myelopoiesis in the acquired immunodeficiency syndrome. New England Journal of Medicine 317:593–598
     [Google Scholar]
  14. Ho D. D., Rota T. R., Hirsch M. S. 1986; Infection of monocytes/macrophages by human T lymphotropic virus type III. Journal of Clinical Investigation 77:1712–1715
     [Google Scholar]
  15. Holland C. A., Chada S., Wright J., Whitney C., Harigaya K., Greenberger J. S., Newburger P. 1989; Differentiation of human hematopoietic cells increases expression of a gene transferred by a retroviral vector. Journal of Leukocyte Biology 46:221–229
     [Google Scholar]
  16. Kunsch C., Wigdahl B. 1989; Transient expression of human immunodeficiency virus type 1 genome results in a nonproductive infection in human fetal dorsal root ganglia glial cells. Virology 173:715–722
     [Google Scholar]
  17. Leiderman I. Z., Greenberg M. L., Adelsberg B. R., Siegal F. P. 1987; A glycoprotein inhibitor of in vitro granulopoiesis associated with AIDS. Blood 70:1267–1272
     [Google Scholar]
  18. Levy J. A., Shimabukuro J., McHugh T., Casavant C., Stites D., Oshiro L. 1985; AIDS-associated retrovirus (ARV) can productively infect other cells besides human T helper cells. Virology 147:441–449
     [Google Scholar]
  19. McElrath M. J., Pruett J. E., Cohn Z. A. 1989; Mononuclear phagocytes of blood and bone marrow: comparative roles as viral reservoirs in human immunodeficiency virus type I infections. Proceedings of the National Academy of Sciences, U.S.A. 86:675–679
     [Google Scholar]
  20. Meltzer M. S., Skillman D. R., Gomatos P. J., Kalter D. C., Gendelman H. E. 1990; Role of mononuclear phagocytes in the pathogenesis of human immunodeficiency virus infection. Annual Review of Immunology 8:169–194
     [Google Scholar]
  21. Newburger P. E., Speier C., Borregaard N., Walsh C. E., Whitin J. C., Simons E. R. 1984; Development of the superoxide-generating system during differentiation of the HL-60 human promyelocytic leukemia cell line. Journal of Biological Chemistry 259:3771–3776
     [Google Scholar]
  22. Pautrat G., Suzan M., Salaun D., Corbeau P., Allasia C., Morel G., Filippi P. 1990; Human immunodeficiency virus type 1 infection of U937 cells promotes cell differentiation and a new pathway of viral assembly. Virology 179:749–758
     [Google Scholar]
  23. Pauza C. D., Galindo J., Richman D. D. 1988; Human immunodeficiency virus infection of monoblastoid cells: cellular differentiation determines the pattern of virus replication. Journal of Virology 62:3558–3564
     [Google Scholar]
  24. Petit A. J. C., Terpstra F. G., Miedema F. 1987; Human immunodeficiency virus infection down-regulates HLA class II expression and induces differentiation in promonocytic U937 cells. Journal of Clinical Investigation 79:1883–1889
     [Google Scholar]
  25. Prince H., Moody D., Shubin B., Fahey J. L. 1985; Defective monocyte function in acquired immune deficiency syndrome (AIDS): evidence from a monocyte-dependent T-cell proliferative system. Journal of Clinical Immunology 5:21–25
     [Google Scholar]
  26. Roulston A., D’Addario M., Boulerice F., Caplan S., Wainberg M. A., Hiscott J. 1992; Induction of monocytic differentiation and NF-kB-like activities by human immunodeficiency virus 1 infection of myelomonoblastic cells. Journal of Experimental Medicine 175:751–763
     [Google Scholar]
  27. Salahuddin S. Z., Markham P. D., Wong-Staal F., Franchini G., Kalyanaraman V. S., Gallo R. C. 1983; Restricted expression of human T-cell leukemia-lymphoma virus (HTLV) in transformed human umbilical cord blood lymphocytes. Virology 129:51–64
     [Google Scholar]
  28. Spivak J. L., Bender B. S., Quinn T. C. 1984; Hematologic abnormalities in the acquired immune deficiency syndrome. American Journal of Medicine 77:224–228
     [Google Scholar]
  29. Srivastava K. K., Fernandez-Larsson R., Zinkus D. M., Robinson H. L. 1991; HIV-1-NL4-3 replication in four T-cell lines: rate/efficiency of entry, a major determinant of permissiveness. Journal of Virology 65:390–392
     [Google Scholar]
  30. Treacy M., Lai L., Costello C., Clark A. 1987; Peripheral blood and bone marrow abnormalities in patients with HIV related disease. British Journal of Haematology 65:289–294
     [Google Scholar]
  31. Tucker K. A., Lilly M. B., Heck L. Jr, Rado T. A. 1987; Characterization of a new human diploid myeloid leukemia cell line (PLB-985) with granulocytic and monocytic differentiating capacity. Blood 70:372–378
     [Google Scholar]
  32. Yam L. T., Li C. Y., Crosby W. H. 1971; Cytochemical identification of monocytes and granulocytes. American Journal of Clinical Pathology 55:283–290
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-12-3257
Loading
Human immunodeficiency virus type 1-infected HL-60 cells are capable of both monocytic and granulocytic differentiation
J. Gen. Virol. 73, 3257 (1992); https://doi.org/10.1099/0022-1317-73-12-3257
/content/journal/jgv/10.1099/0022-1317-73-12-3257
/content/journal/jgv/10.1099/0022-1317-73-12-3257
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error