1887

Abstract

The purpose of this study was to construct an intergenic transfer vector which can be used for the generation of recombinant myxoma viruses (MVs) expressing a foreign gene insert. Recombinant MVs expressing the gene were constructed by transfection of MV-infected rabbit cells with a transfer expression vector, and isolated under growth conditions selecting for transient expression of the gene. The effect of inserting foreign DNA sequences between the viral thymidine kinase gene and open reading frame MF8a upon the transcription of these genes was investigated.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-12-3241
1992-12-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/12/JV0730123241.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-12-3241&mimeType=html&fmt=ahah

References

  1. Bertholet C., Drillien R., Witter R. 1985; One hundred base pairs of 5′ flanking sequence of a vaccinia virus late gene are sufficient to temporally regulate late transcription. Proceedings of the National Academy of Sciences, U.S.A. 82:2096–2100
    [Google Scholar]
  2. Bouvier M. 1954; Quelques remarques sur la myxomatose. Epizootie de 1949 au Brésil (région de Campinas, est Sao-Paulo). Bulletin de l’Office International des Epizooties 46:76–77
    [Google Scholar]
  3. Boyle D. B., Coupar B. E. H. 1988; A dominant selectable marker for the construction of recombinant poxviruses. Gene 65:123–128
    [Google Scholar]
  4. Buller R. M. L., Smith G. L., Cremer K., Notkins A. L., Moss B. 1985; Decreased virulence of recombinant vaccinia virus expression vectors is associated with a thymidine kinase-negative phenotype. Nature, London 317:813–815
    [Google Scholar]
  5. Buller R. M. L., Chakrabarti S., Cooper J. A., Twardzik D. R., Moss B. 1988; Deletion of the vaccinia virus growth factor gene reduces virus virulence. Journal of Virology 62:866–874
    [Google Scholar]
  6. Chakrabarti S., Brechling K., Moss B. 1985; Vaccinia virus expression vector: coexpression of β-galactosidase provides visual screening of recombinant virus plaques. Molecular and Cellular Biology 5:3403–3409
    [Google Scholar]
  7. Coupar B. E. H., Andrew M. E., Both G. W., Boyle D. B. 1986; Temporal regulation of influenza hemagglutinin expression in vaccinia virus recombinants and effects on the immune system. European Journal of Immunology 16:1479–1487
    [Google Scholar]
  8. Davison A. J., Moss B. 1989; Structure of vaccinia virus early promoters. Journal of Molecular Biology 210:749–769
    [Google Scholar]
  9. Davison A. J., Moss B. 1990; New vaccinia virus recombination plasmids incorporating a synthetic late promoter for high level expression of foreign proteins. Nucleic Acids Research 14:4285–4286
    [Google Scholar]
  10. Falkner F. G., Moss B. 1988; Escherichia coli gpt gene provides dominant selection for vaccinia virus open reading frame expression vectors. Journal of Virology 62:1849–1854
    [Google Scholar]
  11. Falkner F. G., Moss B. 1990; Transient dominant selection of recombinant vaccinia viruses. Journal of Virology 64:3108–3111
    [Google Scholar]
  12. Fenner F. 1976; Classification and nomenclature of viruses. Second Report of the International Committee on Taxonomy of Viruses. Intervirology 7:1–116
    [Google Scholar]
  13. Fenner F. 1983; Biological control, as exemplified by smallpox eradication and myxomatosis. Proceedings of the Royal Society of London, Series B 218:259–285
    [Google Scholar]
  14. Fenner F., Ratcliffe F. N. 1965 Myxomatosis Cambridge: Cambridge University Press;
    [Google Scholar]
  15. Franke C. A., Rice C. M., Strauss J. H., Hruby D. E. 1985; Neomycin resistance as a dominant selectable marker for selection and isolation of vaccinia virus recombinants. Molecular and Cellular Biology 5:1918–1924
    [Google Scholar]
  16. Goebel S. J., Johnson G. P., Perkus M. E., Davis S. W., Winslow J. P., Paoletti E. 1990; The complete DNA sequence of vaccinia virus. Virology 179:247–266
    [Google Scholar]
  17. Golini F., Kates J. R. 1984; Transcriptional and translational analysis of a strongly expressed early region of the vaccinia virus genome. Journal of Virology 49:459–470
    [Google Scholar]
  18. Hänggi M., Bannwarth W., Stunnenberg H. G. 1986; Conserved TAAAT motif in vaccinia virus promoters: overlapping TATA box and site of transcription initiation. EMBO Journal 5:1071–1076
    [Google Scholar]
  19. Herman G. E., O’Brien W. E., Beaudel A. L. 1986; An E. coli β-galactosidase cassette suitable for study of eukaryotic expression. Nucleic Acids Research 14:7130
    [Google Scholar]
  20. Jackson R. J., Bults H. G. 1992; The myxoma virus thymidine kinase gene: sequence and transcriptional mapping. Journal of General Virology 73:323–328
    [Google Scholar]
  21. Macaulay C., McFadden G. 1989; Tumorigenic poxviruses: characterization of an early promoter from Shope fibroma virus. Virology 172:237–246
    [Google Scholar]
  22. Mackett M., Smith G. L., Moss B. 1982; Vaccinia virus: a selectable eukaryotic cloning and expression vector. Proceedings of the National Academy of Sciences, U.S.A. 79:7415–7419
    [Google Scholar]
  23. Opgenorth A., Strayer D., Upton C., McFadden G. 1992; Deletion of the growth factor gene related to EGF and TGFα reduces virulence of malignant rabbit fibroma virus. Virology 186:175–191
    [Google Scholar]
  24. Panicali D., Grzelecki A., Huang C. 1986; Vaccinia virus vectors utilizing the β-galactosidase assay for rapid selection of recombinant viruses and measurement of gene expression. Gene 47:193–199
    [Google Scholar]
  25. Russell R. J., Robbins S. J. 1989; Cloning and molecular characterization of the myxoma virus genome. Virology 170:147–159
    [Google Scholar]
  26. Schwer B., Visca P., Vos J. C., Stunnenberg H. G. 1987; Discontinuous transcription or RNA processing of vaccinia virus late messengers results in a 5′ poly(A) leader. Cell 50:163–169
    [Google Scholar]
  27. Tsao H., Liu G.-Q., Ruan L., Chu C.-M. 1988; Construction and application of plasmids containing bidirectional promoters of vaccinia virus. Journal of Virology 62:4832–4834
    [Google Scholar]
  28. Upton C., Macen J. L., Wishart D. S., McFadden G. 1990; Myxoma virus and malignant fibroma rabbit virus encode a serpin-like protein important for virus virulence. Virology 179:618–631
    [Google Scholar]
  29. Upton C., Macen J. L., Schreiber M., McFadden G. 1991; Myxoma virus expresses a secreted protein with homology to the tumor necrosis factor receptor gene family that contributes to viral virulence. Virology 184370–382
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-12-3241
Loading
/content/journal/jgv/10.1099/0022-1317-73-12-3241
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error