1887

Abstract

Protein kinases induced by nuclear polyhedrosis virus in pupae of the silkworm were examined by activity gel analysis using phosvitin as a protein substrate. The method involved PAGE of the soluble fraction from pupae under native conditions and in the presence of SDS, followed by renaturation of proteins and recovery of protein kinase activity in the intact gel. A novel protein kinase able to phosphorylate phosvitin was detected in the infected pupae from 2 days post-infection. This enzyme was not present in uninfected silkworms at any stage of the pupal period. The novel kinase activity was found by SDS-PAGE to be associated with a single polypeptide with an apparent of 50K. However, on electrophoresis under native conditions its activity was associated with a set of polypeptides with similar but not identical electrophoretic mobilities. Microheterogeneity of the catalytically active polypeptides suggests that the virus-induced protein kinase undergoes post-translational modification during the course of infection.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-12-3231
1992-12-01
2021-10-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/12/JV0730123231.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-12-3231&mimeType=html&fmt=ahah

References

  1. Blair G. E., Russell W. C. 1978; Identification of a protein kinase activity associated with human adenoviruses. Virology 86:157–166
    [Google Scholar]
  2. Britt W. J., Auger D. 1986; Human cytomegalovirus virion-associated protein with kinase activity. Journal of Virology 59:185–188
    [Google Scholar]
  3. Frame M. C., Purves F. C., McGeoch D. J., Marsden H. S., Leader D. P. 1987; Identification of the herpes simplex virus protein kinase as the product of viral gene US3. Journal of General Virology 68:2699–2704
    [Google Scholar]
  4. Francki R. I. B., Fauquet C. M., Knudson D. L., Brown F. (editors) 1991 Classification and Nomenclature of Viruses New York: Springer-Verlag;
    [Google Scholar]
  5. Hatanaka M., Twiddy E., Gilden W. V. 1972; Protein kinase associated with RNA tumor viruses and other budding RNA viruses. Virology 47:536–538
    [Google Scholar]
  6. Kelly D. C., Lescott T. 1984; Baculovirus replication: phosphorylation of polypeptides synthesized in Trichoplusia ni nuclear polyhedrosis virus-infected cells. Journal of General Virology 65:1183–1191
    [Google Scholar]
  7. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  8. Lemaster S., Roizman B. 1980; Herpes simplex virus phosphoproteins. II. Characterization of the virion protein kinase and of the polypeptides phosphorylated in the virion. Journal of Virology 35:798–811
    [Google Scholar]
  9. Maeda S., Majima K. 1990; Molecular cloning and physical mapping of the genome of Bombyx mori nuclear polyhedrosis virus. Journal of General Virology 71:1851–1855
    [Google Scholar]
  10. Maruniak J. E., Summers M. D. 1981; Autographa californica nuclear polyhedrosis virus phosphoproteins and synthesis of intracellular proteins after virus infection. Virology 109:25–34
    [Google Scholar]
  11. Mikhailov V. S., Ataeva J. O., Marlyev K. A., Kullyev P. K. 1986; Changes in DNA polymerase activities in pupae of the silkworm Bombyx mori after infection with nuclear polyhedrosis virus. Journal of General Virology 67:175–179
    [Google Scholar]
  12. Miller L. K., Adang M. J., Browne D. 1983; Protein kinase activity associated with the extracellular and occluded forms of the baculovirus Autographa californica nuclear polyhedrosis virus. Journal of Virology 46:275–278
    [Google Scholar]
  13. Onodera K., Komano T., Himeno M., Sakai F. 1965; The nucleic acid of nuclear-polyhedrosis virus of the silkworm. Journal of Molecular Biology 13:532–539
    [Google Scholar]
  14. Purves F. C., Katan M., Leader D. P. 1987a; Complete purification of the pseudorabies virus protein kinase. European Journal of Biochemistry 167:507–512
    [Google Scholar]
  15. Purves F. C., Longnecker R. M., Leader D. P., Roizman B. 1987b; Herpes simplex virus 1 protein kinase is encoded by open reading frame US3 which is not essential for virus growth in cell culture. Journal of Virology 61:2896–2901
    [Google Scholar]
  16. Roux L., Kolakofsky D. 1974; Protein kinase associated with Sendai virions. Journal of Virology 13:545–547
    [Google Scholar]
  17. Rubenstein A. S., Gravell M., Darlington R. 1972; Protein kinase in enveloped herpes simplex virions. Virology 50:287–290
    [Google Scholar]
  18. Somogyi T., Michelson S., Masse M.-J. O. 1990; Genomic location of a human cytomegalovirus protein with protein kinase activity (PK68). Virology 174:276–285
    [Google Scholar]
  19. Stevely W. S., Katan M., Stirling V., Smith G., Leader D. P. 1985; Protein kinase activities associated with the virions of pseudorabies and herpes simplex virus. Journal of General Virology 66:661–673
    [Google Scholar]
  20. Tan K. B., Sokol F. 1974; Virion-bound protein kinase in Semliki forest and Sindbis viruses. Journal of Virology 13:1245–1253
    [Google Scholar]
  21. Wilson M. E., Consign R. A. 1985a; Characterization of a protein kinase activity associated with purified capsids of the granulosis virus infecting. Plodia interpunctella. Virology 143:516–525
    [Google Scholar]
  22. Wilson M. E., Consign R. A. 1985b; Function of a protein kinase activity associated with purified capsids of the granulosis virus infecting Plodia interpunctella . Virology 143:526–535
    [Google Scholar]
  23. Zemskov E. A., Abramova E. B., Mikhailov V. S. 1991; Virus-induced phosphoproteins in silkworm pupae infected with nuclear polyhedrosis virus. Biokhimiya (in Russian) 562207–2217
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-12-3231
Loading
/content/journal/jgv/10.1099/0022-1317-73-12-3231
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error