RT Journal Article SR Electronic(1) A1 Ziegler, A. A1 Natsuaki, T. A1 Mayo, M. A. A1 Jolly, C. A. A1 Murant, A. F.YR 1992 T1 The nucleotide sequence of RNA-1 of raspberry bushy dwarf virus JF Journal of General Virology, VO 73 IS 12 SP 3213 OP 3218 DO https://doi.org/10.1099/0022-1317-73-12-3213 PB Microbiology Society, SN 1465-2099, AB Raspberry bushy dwarf virus (RBDV) has isometric, 33 nm diameter particles and a bipartite RNA genome. Sequencing of the larger component (RNA-1) showed that it consists of 5449 nucleotides and contains one large open reading frame encoding a putative translation product with a calculated M r of 190000. Comparisons of this polypeptide with non-structural proteins of other plant viruses revealed significant homologies with those of alfalfa mosaic virus (AlMV), brome mosaic virus (BMV), cucumber mosaic virus (CMV) and tobacco mosaic virus (TMV). Thus RBDV belongs to the supergroup of ‘Sindbis-like’ plant viruses. The translation product of RBDV RNA-1 contains motifs characteristic of proteins with polymerase, methyltransferase and helicase activities, suggesting that this protein is involved in the replication of the viral RNA. Thus in RBDV, as in TMV, all three functional domains are combined in the single protein, whereas in AlMV, BMV and CMV these domains are distributed over the proteins encoded by RNA-1 and RNA-2. These findings support the idea that RBDV should be placed in a distinct virus genus for which the name idaeovirus has been proposed., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-73-12-3213