1887

Abstract

Raspberry bushy dwarf virus (RBDV) has isometric, 33 nm diameter particles and a bipartite RNA genome. Sequencing of the larger component (RNA-1) showed that it consists of 5449 nucleotides and contains one large open reading frame encoding a putative translation product with a calculated of 190000. Comparisons of this polypeptide with non-structural proteins of other plant viruses revealed significant homologies with those of alfalfa mosaic virus (AlMV), brome mosaic virus (BMV), cucumber mosaic virus (CMV) and tobacco mosaic virus (TMV). Thus RBDV belongs to the supergroup of ‘Sindbis-like’ plant viruses. The translation product of RBDV RNA-1 contains motifs characteristic of proteins with polymerase, methyltransferase and helicase activities, suggesting that this protein is involved in the replication of the viral RNA. Thus in RBDV, as in TMV, all three functional domains are combined in the single protein, whereas in AlMV, BMV and CMV these domains are distributed over the proteins encoded by RNA-1 and RNA-2. These findings support the idea that RBDV should be placed in a distinct virus genus for which the name idaeovirus has been proposed.

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1992-12-01
2024-03-29
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References

  1. Ahlquist P., Janda M. 1984; cDNA cloning and in vitro transcription of complete brome mosaic virus genome. Molecular and Cellular Biology 4:2876–2882
    [Google Scholar]
  2. Ahlquist P., Dasgupta R., Kaesberg P. 1984; Nucleotide sequence of the brome mosaic virus genome and its implications for viral replication. Journal of Molecular Biology 172:369–383
    [Google Scholar]
  3. Ahlquist P., Strauss E. G., Rice C. M., Strauss J. H., Haseloff J., Zimmern D. 1985; Sindbis virus proteins nsP1 and nsP2 contain homology to nonstructural proteins from several plant viruses. Journal of Virology 53:536–542
    [Google Scholar]
  4. Allison R. F., Janda M., Ahlquist P. 1988; Infectious in vitro transcripts from cowpea chlorotic mottle virus cDNA clones and exchange of individual RNA components with brome mosaic virus. Journal of Virology 62:3581–3588
    [Google Scholar]
  5. Allison R. F., Janda M., Ahlquist P. 1989; Sequence of cowpea chlorotic mottle virus RNAs 2 and 3 and evidence of a recombinant event during bromovirus evolution. Virology 172:321–330
    [Google Scholar]
  6. Barnett O. W., Murant A. F. 1970; Host range, properties and purification of raspberry bushy dwarf virus. Annals of Applied Biology 65:435–449
    [Google Scholar]
  7. Cornelissen B. J. C., Brederode F. T., Moormann R. J. M., Bol J. F. 1983a; Complete nucleotide sequence of alfalfa mosaic virus RNA 1. Nucleic Acids Research 11:1253–1265
    [Google Scholar]
  8. Cornelissen B. J. C., Brederode F. T., Veeneman G. H., van Boom J. H., Bol J. F. 1983b; Complete nucleotide sequence of alfalfa mosaic virus RNA 2. Nucleic Acids Research 11:3019–3025
    [Google Scholar]
  9. Cornelissen B. J. C., Linthorst H. J. M., Brederode F. T., Bol J. F. 1986; Analysis of the genome structure of tobacco rattle virus strain PSG. Nucleic Acids Research 14:2157–2169
    [Google Scholar]
  10. DeBorde D. C., Naeve C. W., Herlocher M. L., Maassab H. F. 1986; Resolution of a common RNA sequencing ambiguity by terminal deoxynucleotidyl transferase. Analytical Biochemistry 157:275–282
    [Google Scholar]
  11. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  12. Dzianott A. M., BujaRski J. J. 1991; The nucleotide sequence and genome organization of the RNA-1 segment in two bromo-viruses: broad bean mottle virus and cowpea chlorotic mottle virus. Virology 185:553–562
    [Google Scholar]
  13. Francki R. I. B., Fauquet C. M., Knudson D. L., Brown F. (editors) 1991; Classification and Nomenclature of Viruses: Fifth Report of the International Committee on Taxonomy of Viruses. Archives of Virology Supplementum 2 New York: Springer-Verlag;
    [Google Scholar]
  14. Goelet P., Lomonossoff G. P., Butler P. J. G., Akam M. E., Gait M. J., Karn J. 1982; Nucleotide sequence of tobacco mosaic virus RNA. Proceedings of the National Academy of Sciences, U.S.A. 79:5818–5822
    [Google Scholar]
  15. Goldbach R., Wellink J. 1988; Evolution of plus-strand RNA viruses. Intervirology 29:260–267
    [Google Scholar]
  16. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  17. Gupta K. C., Kingsbury D. W. 1984; Complete sequences of the intergenic and mRNA start signals in the Sendai virus genome: homologies with the genome of vesicular stomatitis virus. Nucleic Acids Research 12:3829–3841
    [Google Scholar]
  18. Haseloff J., Goelet P., Zimmern D., Ahlquist P., Dasgupta R., Kaesberg P. 1984; Striking similarities in amino acid sequence among nonstructural proteins encoded by RNA viruses that have dissimilar genomic organization. Proceedings of the National Academy of Sciences, U.S.A. 81:4358–4362
    [Google Scholar]
  19. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  20. Mayo M. A., Robinson D. J., Jolly C. A., Hyman L. 1989; Nucleotide sequence of potato leafroll luteovirus RNA. Journal of General Virology 70:1037–1051
    [Google Scholar]
  21. Mayo M. A., Jolly C. A., Murant A. F., Raschke J. H. 1991; Nucleotide sequence of raspberry bushy dwarf virus RNA-3. Journal of General Virology 72:469–472
    [Google Scholar]
  22. Mi S., Durbin R., Huang H. V., Rice C. M., Stollar V. 1989; Association of the Sindbis virus RNA methyltransferase activity with the nonstructural protein nsP1. Virology 170:385–391
    [Google Scholar]
  23. Murant A. F. 1976; Raspberry bushy dwarf virus. CMI/AAB Descriptions of Plant Viruses no 165
    [Google Scholar]
  24. Murant A. F. 1987; Raspberry bushy dwarf. In Virus Diseases of Small Fruits, United States Department of Agriculture, Agriculture Handbook No. 631 pp 229–234 Edited by Converse R. H. Washington: United States Government Printing Office;
    [Google Scholar]
  25. Murant A. F., Mayo M. A., Raschke J. H. 1986; Some biochemical properties of raspberry bushy dwarf virus. Acta horticulturae 186:23–30
    [Google Scholar]
  26. Natsuaki T., Mayo M. A., Jolly C. A., Murant A. F. 1991; Nucleotide sequence of raspberry bushy dwarf virus RNA-2: a bicistronic component of a bipartite genome. Journal of General Virology 72:2183–2189
    [Google Scholar]
  27. Quadt R., Rosdorff H. J. M., Hunter T. W., Jaspars E. M. J. 1991; Analysis of the protein composition of alfalfa mosaic virus RNA-dependent RNA polymerase. Virology 182:309–315
    [Google Scholar]
  28. Rezaian M. A., Williams R. H., Gordon K., Gould A. R., Symons R. H. 1984; Nucleotide sequence of cucumber mosaic virus RNA2 reveals a translation product significantly homologous to corresponding protein of other viruses. European Journal of Biochemistry 143:277–284
    [Google Scholar]
  29. Rezaian M. A., Williams R. H., Symons R. H. 1985; Nucleotide sequence of cucumber mosaic virus RNA1. Presence of a sequence complementary to part of the viral satellite RNA and homologies with other viral RNAs. European Journal of Biochemistry 150:331–339
    [Google Scholar]
  30. Romero J., Dzianott A. M., Bujarski J. J. 1992; The nucleotide sequence and genome organization of the RNA2 and RNA3 segments in broad bean mottle virus. Virology 187:671–681
    [Google Scholar]
  31. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A. 74:5463–5467
    [Google Scholar]
  32. Staden R. 1982; Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing. Nucleic Acids Research 10:4731–4751
    [Google Scholar]
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