1887

Journal of General Virology header logo

Volume 73, Issue 12

Equilibrium and kinetic analysis of nuclear polyhedrosis virus attachment to different insect cell lines Free

Abstract

The kinetic and equilibrium attachment of nuclear polyhedrosis virus (AcMNPV) to seven insect cell lines was evaluated. Kinetic experiments revealed differences of up to 10-fold in the infection rates among cell lines. Equilibrium binding also varied between cell lines and was saturable. The Tn 5B1-4 and Tn F cell lines had the highest virus binding affinities and infection rates and exhibited diffusion-limited attachment. The rate of infection appears to be limited by the rate of attachment. For the Tn 5B1-4 cells the physical to infective particle ratio for AcMNPV was 5.3. From the Scatchard analyses, the cell lines Tn 5B1-4 and Tn F displayed affinities of 2.35 × 10 and 1.60 × 10 , respectively, with 6000 and 13700 binding sites per cell. The insect cell line Hz 1075, which is not susceptible to AcMNPV infection, displayed a much lower, but saturable, binding of AcMNPV with 900 sites/cell and an affinity of 1.1 × 10 . Unlabelled AcMNPV, but not MNPV could compete with labelled AcMNPV for binding sites. There were 93 to 96% reductions in virus cell binding following pretreatments of cells with three proteases, suggesting the involvement of a cellular protein component in virus binding. Tunicamycin, an inhibitor of -linked glycosylation and expression of some membrane proteins on the cell surface, reduced virus binding in a dose-dependent manner suggesting a role for glycoprotein(s) in binding. However there was no evidence for the direct involvement of oligosaccharides in attachment. Metabolic inhibitors of oligosaccharide trimming and competition binding assays using simple sugars caused no measurable reductions in virus binding. These findings suggest that AcMNPV attachment to insect cells is receptor-mediated via a glycoprotein component(s); the direct involvement of oligosaccharide moieties in binding is unlikely.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1992
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-12-3185
1992-12-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/12/JV0730123185.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-12-3185&mimeType=html&fmt=ahah

References

  1. Alcami A., Carrascosa A. L., Vinuela E. 1989; Saturable binding sites mediate the entry of African swine fever virus in Vero cells. Virology 168:393–398
     [Google Scholar]
  2. Atkins P. W. 1982 Physical Chemistry pp 809–856 San Francisco: W. H. Freeman;
     [Google Scholar]
  3. Basak S., Compans R. W. 1989; Polarized entry of canine parvovirus in an epithelial cell line. Journal of Virology 63:3164–3167
     [Google Scholar]
  4. Dalgleish A. G., Beverley P. C. L., Clapham P. R., Crawford D. H., Greaves M. F., Weiss R. A. 1984; The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus. Nature, London 312:763–767
     [Google Scholar]
  5. De Gooijer C. D., Van Lier F. L. J., van den End E. J., Vlak J. M., Tramper J. 1989; A model for baculovirus production with continuous insect cell cultures. Applied Microbiology and Biotechnology 30:497–501
     [Google Scholar]
  6. Elbein A. D. 1987; Glycosylation inhibitors for N-linked glycoproteins. Methods in Enzymology 138:661–709
     [Google Scholar]
  7. Epstein R. L., Powers M. L., Rogart R. B., Weiner H. L. 1984; Binding of 125I-labeled reovirus to cell surface receptors. Virology 133:46–55
     [Google Scholar]
  8. Evans H. F. 1986; Ecology and epizootiology of baculoviruses. In The Biology of Baculoviruses vol 2 Practical Application for Insect Control, pp. 89–132 Edited by Granados R. R., Federici B. A. Boca Raton: CRC Press;
     [Google Scholar]
  9. Fries E., Helenius A. 1979; Binding of Semliki Forest virus and its spike glycoproteins to cells. European Journal of Biochemistry 97:213–220
     [Google Scholar]
  10. Goodwin R. H., Tompkins G. J., Gettig R. R., Adams J. R. 1982; The characterization and culture of virus replicating continuous insect cell lines from the bollworm, Heliothis zea (Boddie). In Vitro 18:843–850
     [Google Scholar]
  11. Granados R. R., Naughton M. 1976; Replication of Amsacta moorei entomopoxvirus and Autographa californica nuclear polyhe-drosis virus in hemocyte cell lines from Estigmene acrea . In Invertebrate Tissue Culture, Applications in Medicine, Biology, and Agriculture pp 379–389 Edited by Kurstak E., Maramorosch K. New York: Academic Press;
     [Google Scholar]
  12. Hink W. F. 1970; Established insect cell line from the cabbage looper. Trichoplusia ni. Nature, London 266:466–467
     [Google Scholar]
  13. Jarvis D. L., Summers M. D. 1989; Glycosylation and secretion of human tissue plasminogen activator in recombinant baculovirus-infected insect cells. Molecular and Cellular Biology 9:214–223
     [Google Scholar]
  14. Klatzmann D., Champagne E., Chamaret S., Gruest J., Guetard D., Hercend T., Gluckman J. C., Montaignier L. 1984; T-Lymphocyte T4 molecule behaves as the receptor for human retrovirus LAV. Nature, London 312:767–768
     [Google Scholar]
  15. Labarca C., Paigen K. 1980; A simple, rapid, and sensitive DNA assay procedure. Analytical Biochemistry 102:344–352
     [Google Scholar]
  16. Layne S. P., Spouge J. L., Dembo M. 1989; Quantifying the infectivity of human immunodeficiency virus. Proceedings of the National Academy of Sciences, U.S.A. 86:4644–4648
     [Google Scholar]
  17. Luckow V. A. 1990; Cloning and expression of heterologous genes in insect cells with baculovirus vectors. In Recombinant DNA Technology and Applications pp 2–25 Edited by Ho C., Prokop A., Bajpai R. New York: McGraw-Hill;
     [Google Scholar]
  18. Mettenleiter T. C., Zsak L., Zuckermann F., Sugg N., Kern H., Ben-Porat T. 1990; Interaction of glycoprotein gIII with a cellular heparinlike substance mediates adsorption of pseudorabies virus. Journal of Virology 64:278–286
     [Google Scholar]
  19. Oker-Blom C., Pettersson R. F., Summers M. D. 1989; Baculovirus polyhedrin promoter-directed expression of rubella virus envelope proteins, E1 and E2, in Spodoptera frugiperda cells. Virology 172:82–91
     [Google Scholar]
  20. Paulson J. C., Rogers G. N., Murayama J.-I., Sze G., Martin E. 1986; Biological implications of influenza virus receptor specificity. In Virus Attachment and Entry into Cells. Proceedings of the American Society for Microbiology Conference, Philadelphia pp 144–151 Edited by Crowell R. L., Lonberg-Holm K. Washington, D.C.: American Society for Microbiology;
     [Google Scholar]
  21. Roberts T. E. 1989; Fatty acid acylated baculovirus polypeptides and aspects of intracellular protein processing. Ph,. D. thesis Queen’s University; Kingston, Ontario, Canada:
     [Google Scholar]
  22. Schlegel R., Willingham M. C., Pastan I. H. 1982; Saturable binding sites for vesicular stomatitis virus on the surface of Vero cells. Journal of Virology 43:871–875
     [Google Scholar]
  23. Schwarz R. T., Datema R. 1984; Inhibitors of trimming: new tools in glycoprotein research. Trends in Biological Sciences 9:32–34
     [Google Scholar]
  24. Shoup D., Szabo A. 1982; Role of diffusion in ligand binding to macromolecules and cell-bound receptors. Biophysical Journal 40:33–39
     [Google Scholar]
  25. Smith I. R. L., van Beek N. A. M., Podgwaite J. D., Wood H. A. 1988; Physical map and polyhedrin gene sequence of Lymantria dispar nuclear polyhedrosis virus. Gene 71:97–105
     [Google Scholar]
  26. Smoluchowski M. V. 1917; Versuch einer mathematischen theorie der koagulationskinetic kolloider losungen. Zeitschrift für Physika-lische Chemie 92:129–168
     [Google Scholar]
  27. Summers M. D., Smith G. A. 1987; A manual of methods for baculovirus vectors and insect cell culture procedures. Texas Agricultural Experimental Station Bulletin B1555:1–56
     [Google Scholar]
  28. Taylor H. P., Cooper N. R. 1989; Human cytomegalovirus binding to fibroblasts is receptor-mediated. Journal of Virology 63:3991–3998
     [Google Scholar]
  29. Trimble R. B., Maley F. 1984; Optimizing hydrolysis of N-linked high-mannose oligosaccharides by endo-β-N-acetylglucosaminidase H. Analytical Biochemistry 141:515–522
     [Google Scholar]
  30. Vaughn J. L., Goodwin R. H., Tompkins G. J., McCawley P. 1977; The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera : Noctuidae). In Vitro 13:213–217
     [Google Scholar]
  31. Verdin E. M., King G. L., Maratos-Flier E. 1989; Characterization of a common high-affinity receptor for reovirus serotypes 1 and 3 on endothelial cells. Journal of Virology 63:1318–1325
     [Google Scholar]
  32. Volkman L. E., Summers M. D., Hsieh C.-H. 1976; Occluded and non-occluded nuclear polyhedrosis virus grown in Trichoplusia ni: comparative neutralization, comparative infectivity, and in vitro growth studies. Journal of Virology 19:820–832
     [Google Scholar]
  33. Volkman L. E., Goldsmith K., Hess R. T. 1986; Alternate pathway of entry of budded Autographa californica nuclear polyhedrosis virus: fusion at the plasma membrane. Virology 148:288–297
     [Google Scholar]
  34. Wang X., Kelly D. C. 1985; Baculovirus replication: uptake of Trichoplusia ni nuclear polyhedrosis virus particles by insect cells. Journal of General Virology 66:541–550
     [Google Scholar]
  35. Wickham T. J., Granados R. R., Wood H. A., Hammer D. A., Shuler M. L. 1990; General analysis of receptor-mediated viral attachment to cell surfaces. Biophysical Journal 58:1501–1516
     [Google Scholar]
  36. Wood H. A. 1977; An agar overlay plaque assay method for Autographa californica nuclear polyhedrosis virus. Journal of Invertebrate Pathology 29:304–307
     [Google Scholar]
  37. Wood H. A. 1980; Autographa californica nuclear polyhedrosis virus-induced proteins in tissue culture. Virology 102:21–27
     [Google Scholar]
  38. Wood H. A., Johnston L. B., Burand J. B. 1982; Inhibition of AcNPV replication in high-density Trichoplusia ni cell cultures. Virology 119:245–254
     [Google Scholar]
  39. Wudunn D., Spear P. G. 1989; Initial interaction of herpes simplex virus with cells is binding to heparan sulfate. Journal of Virology 63:52–58
     [Google Scholar]
  40. Wunner W. H., Reagan K. J., Koprowski H. 1984; Characterization of saturable binding sites for rabies virus. Journal of Virology 50:691–697
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-12-3185
Loading
Equilibrium and kinetic analysis of Autographa californica nuclear polyhedrosis virus attachment to different insect cell lines
J. Gen. Virol. 73, 3185 (1992); https://doi.org/10.1099/0022-1317-73-12-3185
/content/journal/jgv/10.1099/0022-1317-73-12-3185
/content/journal/jgv/10.1099/0022-1317-73-12-3185
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error