Restricted replication of vesicular stomatitis virus in T lymphocytes is coincident with a deficiency in a cellular protein kinase required for viral transcription Free

Abstract

Vesicular stomatitis virus (VSV) fails to replicate in mouse T lymphocytes unless the cells have been mitogenically stimulated with concanavalin A (Con A). We have examined the possibility that the failure of VSV to replicate in unstimulated T lymphocytes can be attributed to a deficiency in a host protein kinase which activates the viral P protein by phosphorylation, thus rendering it transcriptionally competent. Soluble extracts were prepared from purified mouse T lymphocytes, with or without prior treatment with Con A. The ability of these extracts to phosphorylate bacterially synthesized P protein of two VSV serotypes was measured . Activity of the protein kinase on the P proteins of the Indiana or New Jersey serotypes of VSV increased, on average 2.4- and 2.1-fold respectively, after treatment of the cells with 3 µg/ml Con A. Higher concentrations of Con A induced proportional increases (up to 10-fold) in the activity of the host protein kinase. Activities of the kinase phosphorylating the P protein in separate populations of CD4- and CD8-containing murine T lymphocytes increased similarly on mitogenic activation. No biochemical or immunological differences were observed between the T cell protein kinase and the previously characterized protein kinase (casein kinase II) from BHK-21 cells. The activity of the kinase that phosphorylates the P protein did not vary in CV-1 cells on treatment with α- or γ-interferon, both of which inhibited VSV replication. Similarly, casein kinase II activities in Raji and SIRC cells, which do not normally support VSV growth, were the same as in BHK-21 cells. Thus restriction of VSV replication in these cells, in contrast to T lymphocytes, was not associated with a deficiency in the host casein kinase II activity.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-12-3125
1992-12-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/12/JV0730123125.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-12-3125&mimeType=html&fmt=ahah

References

  1. Banerjee A. K. 1987a; Transcription and replication of rhabdoviruses. Microbiological Reviews 51:66–87
    [Google Scholar]
  2. Banerjee A. K. 1987b; The transcription complex of vesicular stomatitis virus. Cell 48:363–364
    [Google Scholar]
  3. Banerjee A. K., Moyer S., Rhodes D. 1974; Studies on the in vitro adenylation of RNA by vesicular stomatitis virus. Virology 61:547–558
    [Google Scholar]
  4. Barik S., Banerjee A. K. 1991; Cloning and expression of the vesicular stomatitis virus phosphoprotein gene in Escherichia coli: analysis of phosphorylation status versus transcriptional activity. Journal of Virology 65:1719–1726
    [Google Scholar]
  5. Barik S., Banerjee A. K. 1992a; Sequential phosphorylation of the phosphoprotein of vesicular stomatitis virus by cellular and viral protein kinases is essential for transcription activation. Journal of Virology 66:1109–1118
    [Google Scholar]
  6. Barik S., Banerjee A. K. 1992b; Phosphorylation by cellular casein kinase II is essential for the transcriptional activity of vesicular stomatitis virus phosphoprotein P. Proceedings of the National Academy of Sciences, U.S.A. 89:6570–6574
    [Google Scholar]
  7. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  8. Chattopadhyay D., Banerjee A. K. 1987; Phosphorylation within a specific domain of the phosphoprotein of vesicular stomatitis virus regulates transcription in vitro . Cell 49:407–414
    [Google Scholar]
  9. Epstein M. A., Achong B. G., Barr Y. M., Zajac B., Henle G., Henle W. 1966; Morphological and virological investigations on cultured Burkitt tumor lymphoblasts (strain Raji). Journal of the National Cancer Institute 37:547–551
    [Google Scholar]
  10. Hammond D. C., Haley B. E., Lesnaw J. A. 1992; Identification and characterization of serine/threonine protein kinase activity intrinsic to the L protein of vesicular stomatitis virus New Jersey. Journal of General Virology 73:67–75
    [Google Scholar]
  11. Henle G., Henle W. 1965; Evidence for a persistent viral infection in a cell line derived from Burkitt’s lymphoma. Journal of Bacteriology 89:252–258
    [Google Scholar]
  12. Henle G., Henle W. 1966; Immunofluorescence in cells derived from Burkitt’s lymphoma. Journal of Bacteriology 91:1248–1256
    [Google Scholar]
  13. Hsu C.-H., Morgan E. M., Kingsbury D. W. 1982; Site-specific phosphorylation regulates the transcriptive activity of vesicular stomatitis virus. Journal of Biological Chemistry 260:8990–8995
    [Google Scholar]
  14. Johnson G. P., Herman R. C. 1984; Nonpermissive infection of lymphoblastoid cells by vesicular stomatitis virus. I. Synthesis and function of the viral transcripts. Virus Research 1:259–274
    [Google Scholar]
  15. Julius M. H., Simpson E., Herzenberg L. 1973; A rapid method for the isolation of functional thymus-derived murine lymphocytes. European Journal of Immunology 3:6450649
    [Google Scholar]
  16. Kingsford L., Emerson S. U. 1980; Transcriptional activities of different phosphorylated species of NS protein purified from vesicular stomatitis virions and cytoplasm of infected cells. Journal of Virology 33:1097–1105
    [Google Scholar]
  17. Meisner H., Heller-Harrison R., Buxton J., Czech M. P. 1989; Molecular cloning of the human casein kinase II a subunit. Biochemistry 28:4072–4076
    [Google Scholar]
  18. Muller-Decker K., Amtmann E., Sauer G. 1987; Inhibition of the phosphorylation of the regulatory non-structural protein of vesicular stomatitis virus by an antiviral xanthate compound. Journal of General Virology 68:3045–3056
    [Google Scholar]
  19. Nowakowski M., Bloom B. R., Ehrenfeld E., Summers D. F. 1973; Restricted replication of vesicular stomatitis virus in human lymphoblastoid cells. Journal of Virology 12:1272–1278
    [Google Scholar]
  20. Rudd C. E., Anderson P., Morimoto C., Streuli M., Sclossman S. F. 1989; Molecular interactions, T-cell subsets and a role of the CD4/CD8:p561ck complex in human T cell activation. Immunological Reviews 111:225–266
    [Google Scholar]
  21. Rup B. J., Scott D. W. 1987; VSV replication in normal and transformed T cells, an assay for T suppressor cell function. Cellular Immunology 104:190–194
    [Google Scholar]
  22. Sanchez A., De B. P., Banerjee A. K. 1985; In vitro phosphorylation of NS protein by the L protein of vesicular stomatitis virus. Journal of General Virology 66:1025–1036
    [Google Scholar]
  23. Sauer G., Amtmann E., Melber K., Knapp A., Muller K., Hummel K., Scherm A. 1984; DNA and RNA virus species are inhibited by xanthates, a class of antiviral compounds with unique properties. Proceedings of the National Academy of Sciences, U.S.A. 81:3263–3267
    [Google Scholar]
  24. Sinacore M. S., Lucas-Lenard J. 1982; The effect of the vesicular stomatitis virus-associated protein kinase on mRNA transcription in vitro . Virology 121:404–413
    [Google Scholar]
  25. Thacore H. R., Youngner J. S. 1975; Abortive infection of a rabbit cornea cell line by vesicular stomatitis virus: conversion of productive infection by superinfection with vaccinia virus. Journal of Virology 16:322–329
    [Google Scholar]
  26. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. Proceedings of the National Academy of Sciences, U.S.A. 76:4350–4354
    [Google Scholar]
  27. Webb D. R., Munshi S., Banerjee A. K. 1981; Replication of vesicular stomatitis virus in murine spleen cells: enrichment of the virus-replicating lymphocytes and analysis of replication restriction. Infection and Immunity 32:169–172
    [Google Scholar]
  28. Wethers J. A., Johnson G. P., Schumacher C. L., Herman R. C. 1985; Nonpermissive infection of lymphoblastoid cells by vesicular stomatitis virus. II. Effect on viral morphogenesis. Virus Research 2:345–358
    [Google Scholar]
  29. Winter E., Yamamoto F., Almoguera C., Perucho M. 1985; A method to detect and characterize point mutations in transcribed genes: amplification and over-production of the mutant c-Ki-ras allele in human tumor cells. Proceedings of the National Academy of Sciences, U.S.A. 75:4257–4261
    [Google Scholar]
  30. Witt D. J., Summers D. F. 1980; Relationship between virion-associated kinase-effected phosphorylation and transcription activity of vesicular stomatitis virus. Virology 107:34–39
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-12-3125
Loading
/content/journal/jgv/10.1099/0022-1317-73-12-3125
Loading

Data & Media loading...

Most cited Most Cited RSS feed