1887

Abstract

The 12 nucleotide conserved sequence at the 3′ end of influenza A virion RNA is sufficient to function as a promoter . By introducing point mutations in all 12 positions of this promoter in model RNA templates and studying the efficiency of RNA synthesis , we show that only three nucleotides, residues 9, 10 and 11, are crucial for activity, although other nucleotides play a significant but less important role. Additions or deletions within the promoter are tolerated, resulting in either an increase or a decrease in promoter activity, depending on the mutation introduced; in some cases premature termination is caused. Taking these observations into account, a model for RNA polymerase binding and copying of the promoter is discussed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-12-3115
1992-12-01
2019-11-22
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-12-3115
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error