1887

Abstract

X-ray diffraction from oriented specimens of purified preparations of particles of heracleum latent clostero-virus (HLV) showed that they have a helical arrangement of protein subunits, and that the structure repeats in five helical turns in which there are 5q ± 1 protein subunits, where q is an integer. The pitch of the helix was estimated to be 3.26 (±0.10) nm. Optical diffraction patterns from electron micrographs of HLV particles give an estimated pitch of 3.3 (±0.2) nm and show that the number of subunits in the repeat period is 5q-1, where q has a value of 8 or 9. Optical diffraction from electron micrographs of particles of a second closterovirus, heracleum virus 6, shows that they too have a helical structure which repeats in five turns, in which there are 5q ± 1 protein subunits. The estimated pitch of the primary helix is 3.6 (± 0.2) nm, and the estimate of q is 9.

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/content/journal/jgv/10.1099/0022-1317-73-11-3045
1992-11-01
2023-01-28
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