1887

Abstract

An antiserum was raised against a fusion protein containing the C-terminal half of the protein (P3) encoded by the satellite RNA of grapevine fanleaf virus (GFLV; F13 isolate) and the N-terminal portion of the CI repressor of phage λ. This antiserum specifically recognized P3 synthesized in the wheatgerm translation system and also in infected plants. In these plants, the amount of virus increased for 10 days, then remained constant for up to 21 days, whereas P3 was detected transiently, reaching its maximum on day 10.

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1992-11-01
2024-04-24
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References

  1. Amann E., Brosius J., Ptashne M. 1983; Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli. Gene 25:167–178
    [Google Scholar]
  2. Berna A., Godefroy-Colburn T., Stussi-Garaud C. 1985; Preparation of an antiserum against an in vitro translation product of alfalfa mosaic virus RNA 3. Journal of General Virology 66:1669–1678
    [Google Scholar]
  3. Berna A., Briand J. P., Stussi-Garaud C., Godefroy-Colburn T. 1986; Kinetics of accumulation of the three non-structural proteins of alfalfa mosaic virus in tobacco plants. Journal of General Virology 67:1135–1147
    [Google Scholar]
  4. DeBoer H. A., Comstock L. J., Vasser M. 1983; The tac promoter: a functional hybrid derived from the trp and lac promoters. Proceedings of the National Academy of Sciences, U. S. A. 80:21–25
    [Google Scholar]
  5. Fritsch C., Mayo M. A. 1989; Satellites of plant viruses. In Plant Viruses vol 1 Structure and Replication, pp 289–321 Edited by Mandahar C. L. Boca Raton: CRC Press;
    [Google Scholar]
  6. Fritsch C., Mayo M. A., Murant A. F. 1978; Translation of the satellite RNA of tomato black ring virus in vitro and in tobacco protoplasts. Journal of General Virology 40:587–593
    [Google Scholar]
  7. Fritsch C., Koenig I., Murant A. F., Raschke J. H., Mayo M. A. 1984; Comparisons among satellite RNA species from five isolates of tomato black ring virus and one isolate of myrobalan latent ringspot virus. Journal of General Virology 65:289–294
    [Google Scholar]
  8. Fuchs M. 1989 Le grapevine fanleaf virus, agent du court-noué de la vigne: approche molé culaire de la premunition, structure et expression du RNA satellite Ph. D. thesis Université Louis Pasteur; Strasbourg:
    [Google Scholar]
  9. Fuchs M., Pinck M., Serghini M. A., Ravelonandro M., Walter B., Pinck L. 1989; The nucleotide sequence of satellite RNA in grapevine fanleaf virus, strain F13. Journal of General Virology 70:955–962
    [Google Scholar]
  10. Godefroy-Colburn T., Thivent C., Pinck L. 1985; Translational discrimination between the four RNAs of alfalfa mosaic virus. A quantitative evaluation. European Journal of Biochemistry 147:541–548
    [Google Scholar]
  11. Godefroy-Colburn T., Gagey M.-J., Berna A., Stussi-Garaud C. 1986; A non-structural protein of alfalfa mosaic virus in the walls of infected tobacco cells. Journal of General Virology 67:2233–2239
    [Google Scholar]
  12. Hewitt W. B., Raski D. J., Goheen A. C. 1958; Nematode vector of soil-borne fanleaf virus of grapevines. Phytopathology 48:586–595
    [Google Scholar]
  13. John M., Schmidt J., Wieneke U., Kondorosi E., Kondorosi A., Schell J. 1985; Expression of the nodulation gene nod C of Rhizobium meliloti in Escherichia coli: role of the nod C gene product in nodulation. EMBO Journal 4:2425–2430
    [Google Scholar]
  14. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  15. Liu Y. Y., Hellen C. U. T., Cooper J. I., Bertioli D. J., Coates D., Bauer G. 1990; The nucleotide sequence of a satellite RNA associated with arabis mosaic nepovirus. Journal of General Virology 71:1259–1263
    [Google Scholar]
  16. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Marston F. A. O. 1986; The purification of eucaryotic polypeptides synthesised in Escherichia coli. Biochemical Journal 240:1–12
    [Google Scholar]
  18. Mayo M. A., Barker H., Robinson D. J. 1982; Satellite RNA in particles of strawberry latent ringspot virus. Journal of General Virology 63:417–423
    [Google Scholar]
  19. Mirtaki A., King J. 1989; Protein folding intermediates and inclusion body formation. Bio/Technology 7:690–697
    [Google Scholar]
  20. Niesbach-Klӧsgen U., Guilley H., Jonard G., Richards K. 1990; Immunodetection in vivo of beet necrotic yellow vein virus-encoded proteins. Virology 178:52–61
    [Google Scholar]
  21. Pinck L., Fuchs M., Pinck M., Ravelonandro M., Walter B. 1988; A satellite RNA in grapevine fanleaf virus strain F13. Journal of General Virology 69:233–239
    [Google Scholar]
  22. Ritzenthaler C., Viry M., Pinck M., Margis R., Fuchs M., Pinck L. 1991; Complete nucleotide sequence and genetic organization of grapevine fanleaf nepovirus RNA1. Journal of General Virology 72:2357–2365
    [Google Scholar]
  23. Rubino L., Tousignant M. E., Steger G., Kaper J. M. 1990; Nucleotide sequence and structural analysis of two satellite RNAs associated with chicory yellow mottle virus. Journal of General Virology 71:1897–1903
    [Google Scholar]
  24. Serghini M. A., Fuchs M., Pinck M., Reinbolt J., Walter B., Pinck L. 1990; RNA2 of grapevine fanleaf virus: sequence analysis and coat protein cistron location. Journal of General Virology 71:1433–1441
    [Google Scholar]
  25. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U. S. A. 76:4350–4354
    [Google Scholar]
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