1887

Abstract

Bluetongue virus (BTV) VP6 is often resolved into two closely migrating bands by SDS-PAGE (VP6 and VP6a). RNA segment 9 of BTV-serotype 1 South Africa (encoding VP6) has been cloned as cDNA, and the complete sequence has been determined. Expression of this clone both and in tissue culture produced the same polypeptide doublet as seen previously in extracts from BTV-infected cells. Modification of the cDNA, including the removal of the first initiation codon, demonstrated that the two forms of VP6 are derived from initiation of protein synthesis at two distinct sites and not by post-translational modification.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-11-3023
1992-11-01
2021-10-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/11/JV0730113023.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-11-3023&mimeType=html&fmt=ahah

References

  1. Belsham G. J., Brangwyn J. K. 1990; A region of the 5′ noncoding region of foot-and-mouth disease virus RNA directs efficient internal initiation of protein synthesis within cells: involvement with the role of L protease in translational control. Journal of Virology 64:5389–5395
    [Google Scholar]
  2. Chan W. -T., Penaranda M. E., Crawford S. E., Estes M. K. 1986; Two glycoproteins are produced from the rotavirus neutralization gene. Virology 151:243–252
    [Google Scholar]
  3. Chen E. Y., Seeburg P. H. 1985; Laboratory methods. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA. DNA 4:165–170
    [Google Scholar]
  4. Devaney M. A., Kendall J., Grubman M. J. 1988; Characterisation of a nonstructural phosphoprotein of two orbiviruses. Virus Research 11:151–164
    [Google Scholar]
  5. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  6. Fuerst T. R., Niles E. G., Studier F. W., Moss B. 1986; Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proceedings of the National Academy of Sciences, U. S. A. 83:8122–8126
    [Google Scholar]
  7. Fukusho A., Yu Y., Yamaguchi S., Roy P. 1989; Completion of the sequence of bluetongue virus serotype 10 by the characterization of a structural protein, VP6, and a non-structural protein, NS2. Journal of General Virology 70:1677–1689
    [Google Scholar]
  8. Grubman M. J., Lewis S. A. 1992; Identification and characterization of the structural and nonstructural proteins of African horse sickness virus and determination of the genome coding assignments. Virology 186:444–451
    [Google Scholar]
  9. Grubman M. J., Appleton J. A., Letchworth G. J. 1983; Identification of BTV type 17 genome segments coding for polypeptides associated with virus neutralization and intergroup reactivity. Virology 131:355–366
    [Google Scholar]
  10. Huismans H., Bremer C. W. 1981; Identification of the serotype-specific and group specific antigens of bluetongue virus. Onderstepoort Journal of Veterinary Research 48:51–58
    [Google Scholar]
  11. Huismans H., Van Dijk A. A., Bauskin A. R. 1987; In vitro phosphorylation and purification of a non-structural protein of bluetongue virus with affinity for single-stranded RNA. Journal of Virology 61:3589–3595
    [Google Scholar]
  12. Kozak M. 1986; Bifunctional messenger RNAs in eukaryotes. Cell 47:481–483
    [Google Scholar]
  13. Kozak M. 1989; The scanning model for translation: an update. Journal of Cell Biology 108:229–241
    [Google Scholar]
  14. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  15. Mecham J. O., Dean V. C. 1988; Protein coding assignment for the genome of epizootic haemorrhagic disease virus. Journal of General Virology 69:1255–1262
    [Google Scholar]
  16. Mertens P. P. C., Sangar D. V. 1985; Analysis of the terminal sequences of the genome segments of four orbiviruses. Virology 140:55–67
    [Google Scholar]
  17. Mertens P. P. C., Brown F., Sangar D. V. 1984; Assignment of the genome segments of bluetongue virus type 1 to the proteins which they encode. Virology 135:207–217
    [Google Scholar]
  18. Mertens P. P. C., Burroughs J. N., Anderson J. 1987; Purification and properties of virus particles, infectious subviral particles and cores of bluetongue virus serotypes 1 and 4. Virology 157:375–386
    [Google Scholar]
  19. Rosenberg A. H., Lade B. N., Chui D.-S., Lin S.-W., Dunn J. J., Studier F. W. 1987; Vectors for selective expression of cloned DNAs by T7 RNA polymerase. Gene 56:125–135
    [Google Scholar]
  20. Roy P., Adachi A., Urakawa T., Booth T. F., Thomas C. P. 1990; Identification of BTV VP6 protein as a nucleic acid-binding protein and the localization of VP6 in virus-infected vertebrate cells. Journal of Virology 64:1–8
    [Google Scholar]
  21. Van Dijk A. A., Huismans H. 1988; In vitro transcription and translation of bluetongue virus mRNA. Journal of General Virology 69:573–581
    [Google Scholar]
  22. Verwoerd D. W., Els H. J., De Villiers E. M., Huismans H. 1972; Structure of the bluetongue virus capsid. Journal of Virology 10:783–794
    [Google Scholar]
  23. Wade-Evans A. M., Pan Z. Q., Mertens P. P. C. 1988; Sequence analysis and in vitro expression of a cDNA clone of genome segment 5 from bluetongue virus, serotype 1 from South Africa. Virus Research 11:227–240
    [Google Scholar]
  24. Yang Y.-Y., Li J. K.-K. 1992; Complete genomic sequences of the GP5 protein gene of bluetongue virus serotype 11 and 17. Virus Research 23:163–171
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-11-3023
Loading
/content/journal/jgv/10.1099/0022-1317-73-11-3023
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error