Nuclear localization of dengue 2 virus core protein detected with monoclonal antibodies Free

Abstract

Anti-dengue 2 virus core protein monoclonal antibodies (MAbs) reacted with antigens in the cytoplasm and in, or on, the nucleus of dengue 2 and dengue 4, but not dengue 1, dengue 3, Kunjin or Murray Valley encephalitis virus-infected cells. These MAbs also reacted with the core protein from dengue 1, 2 and 4 virions in Western blots. The antigens detected by these MAbs could not be detected in uninfected or heat-shocked cells, but were first detected in infected cells approximately 32 h post-infection. PEPSCAN epitope mapping suggested that all the MAbs react with a region of the dengue 2 virus core protein (RNTPFNMLKRE) which is adjacent to a putative nuclear localization sequence (KKAR) and spans a possible second site for the initiation of synthesis of core protein (PFN↓MLKR).

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1992-11-01
2024-03-28
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References

  1. Aaskov J. G., Williams L., Fletcher J., Hay R. 1988; Failure of a dengue 1 sub-unit vaccine to protect mice against a lethal dengue virus infection. American Journal of Tropical Medicine and Hygiene 39:511–518
    [Google Scholar]
  2. Anzai T., Ozaki Y. 1969; Intranuclear crystal formation of poliovirus: electron microscopic observations. Experimental and Molecular Pathology 10:176–185
    [Google Scholar]
  3. Buckley A., Gould E. A. 1988; Detection of virus-specific antigen in the nuclei or nucleoli of cells infected with Zika or Langat virus. Journal of General Virology 69:1913–1920
    [Google Scholar]
  4. Burglin T. R., De Robertis E. M. 1987; The nuclear migration signal of Xenopus laevis nucleoplasmin. EMBO Journal 6:2617–2625
    [Google Scholar]
  5. Castle E., Nowak T., Leidner U., Wengler G., Wengler G. 1985; Sequence analysis of the viral core protein and the membrane associated proteins V1 and NV2 of the flavivirus West Nile virus and of the genome sequence for these proteins. Virology 145:227–236
    [Google Scholar]
  6. Chou P. Y., Fasman G. D. 1974; Prediction of protein conformation. Biochemistry 13:222–245
    [Google Scholar]
  7. Coia G., Parker M. D., Speight G., Byrne M. E., Westaway E. G. 1988; Nucleotide and complete amino acid sequences of Kunjin virus: definitive gene order and characteristics of the virus-specified proteins. Journal of General Virology 69:1–21
    [Google Scholar]
  8. Dalgarno L., Trent D. W., Strauss J. H., Rice C. M. 1986; Partial nucleotide sequence of the Murray Valley encephalitis virus genome: comparison of the encoded polypeptides with yellow fever virus structural and non-structural proteins. Journal of Molecular Biology 187:309–323
    [Google Scholar]
  9. Deubel V., Kinney R. M., Trent D. W. 1986; Nucleotide sequence and deduced amino acid sequence of the structural proteins of dengue type 2 virus, Jamaica genotype. Virology 155:365–377
    [Google Scholar]
  10. Dingwall C., Lasky R. A. 1991; Nuclear targeting sequences - a consensus. Trends in Biochemical Sciences 16:478–481
    [Google Scholar]
  11. Garry R. F., Ulug E. T., Bose H. R. 1983; Induction of stress proteins in Sindbis virus and vesicular stomatitis virus-infected cells. Virology 129:319–332
    [Google Scholar]
  12. Geysen H. M., Rodda S. J., Mason T. J., Tribbick G., Schoofs P. G. 1987; Strategies for epitope analysis using peptide synthesis. Journal of Immunological Methods 102:259–274
    [Google Scholar]
  13. Gould E. A., Chanas A. C., Buckley A., Clegg C. S. 1983; Monoclonal immunoglobulin M antibody to JE virus that can react with a nuclear antigen in mammalian cells. Infection and Immunity 41:774–779
    [Google Scholar]
  14. Halstead S. B. 1988; Pathogenesis of dengue: challenges to molecular biology. Science 239:476–481
    [Google Scholar]
  15. Henchal E. A., McCown J. M., Burke D. S., Seguin M. C., Brandt W. E. 1985; Epitopic analysis of antigenic determinants on the surface of dengue-2 virions using monoclonal antibodies. American Journal of Tropical Medicine and Hygiene 34:162–169
    [Google Scholar]
  16. Jackson D. A., Caton A. J., McCready S. J., Cook P. R. 1982; Influenza virus RNA is synthesized at fixed sites in the nucleus. Nature, London 296:366–368
    [Google Scholar]
  17. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  18. Mason P. W., McAda P. C., Mason T. L., Fournier M. J. 1987; Sequence of the dengue-1 virus genome in the region encoding the three structural proteins and the major non-structural protein NS1. Virology 161:262–267
    [Google Scholar]
  19. Milarski K. L., Morimoto R. I. 1989; Mutational analysis of the human HSP70 protein. Distinct domains for nuclear localisation and adenosine triphosphate binding. Journal of Cell Biology 109:1947–1962
    [Google Scholar]
  20. Naeve C. W., Trent D. W. 1978; Identification of Saint Louis encephalitis virus mRNA. Journal of Virology 25:535–545
    [Google Scholar]
  21. Ng M. L., Pedersen J. S., Toh B. H., Westaway E. G. 1983; Immunofluorescent sites in Vero cells infected with the flavivirus Kunjin. Archives of Virology 78:177–190
    [Google Scholar]
  22. Osatomi K., Fuke I., Tsuru D., Shiba T., Sakaki Y., Sumiyoshi H. 1988; Nucleotide sequence of dengue type 3 virus genomic RNA encoding viral structural proteins. Virus Genes 2:99–108
    [Google Scholar]
  23. Parker J. M. R., Guo D., Hodges R. S. 1986; New hydrophilicity scale derived from high-performance liquid chromatography peptide retention data: correlation of predicted surface residues with antigenicity and X-ray-derived accessible sites. Biochemistry 25:5425–5432
    [Google Scholar]
  24. Richardson W. D., Roberts B. L., Smith A. E. 1986; Nuclear location signals in polyoma virus large-T. Cell 44:77–85
    [Google Scholar]
  25. Schagger H., von Jagow G. 1987; Tricine-sodium dodecyl sulphate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Analytical Biochemistry 166:368–379
    [Google Scholar]
  26. Sen K., Honda G., Koyama N., Nishida M., Neki A., Himeno M., KomaNO T. 1988; Cloning and nucleotide sequences of the two 130 kDa insecticidal protein genes of Bacillus thuringiensis var. israelensis. Agricultural and Biological Chemistry 52:873–878
    [Google Scholar]
  27. Speight G., Westaway E. G. 1989; Carboxyterminal analysis of nine proteins specified by the flavirus Kunjin: evidence that only the intracellular core protein is truncated. Journal of General Virology 70:2209–2214
    [Google Scholar]
  28. Swanberg S. L., Wang J. C. 1987; Cloning and sequencing of the Escherichia coli gyrA gene coding for the A subunit of DNA gyrase. Journal of Molecular Biology 197:729–736
    [Google Scholar]
  29. Tadano M., Makino Y., Fukunaga T., Okuno Y., Fukai K. 1989; Detection of dengue 4 virus core protein in the nucleus. I. A monoclonal antibody to dengue 4 virus reacts with the antigen in the nucleus and cytoplasm. Journal of General Virology 70:1409–1415
    [Google Scholar]
  30. Tissieres A., Mitchell H. K., Tracy U. M. 1974; Protein synthesis in salivary glands of Drosophila melanogaster: relation to chromosome puff’s. Journal of Molecular Biology 84:389–398
    [Google Scholar]
  31. Westaway E. G., Ng M. L. 1980; Replication of flaviviruses: separation of membrane translation sites of Kunjin virus proteins and cell protein. Virology 106:107–122
    [Google Scholar]
  32. Young P. R., Chanas A. C., Lee S. R., Gould E. A., Howard C. R. 1987; Localization of an arenavirus protein in the nuclei of infected cells. Journal of General Virology 68:2465–2470
    [Google Scholar]
  33. Zhao B., Mackow E., Buckler-White A., Markoff L., Chanock R. M., Lai C. -J., Makino Y. 1986; Cloning full length dengue type 4 viral DNA sequences: analysis of genes coding for structural proteins. Virology 155:77–88
    [Google Scholar]
  34. Zola H. 1987; Monoclonal Antibodies: A Manual of Techniques. Boca Raton: CRC Press;
    [Google Scholar]
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