1887

Abstract

Thogoto (THO) virus is a tick-borne virus which shares morphological and genetic features with members of the Orthomyxoviridae family although the viral glycoprotein appears to be related to gp64 of baculoviruses. Characterization of THO virus was undertaken to clarify its taxonomic position. Purified virus preparations contained at least six virus-encoded polypeptides with apparent values ranging from 29K to 92K. A 75K polypeptide was identified as an envelope-associated glycoprotein by Triton X-100 and salt dissociation studies, and by proteolytic degradation of the exposed proteins of the virion. By the same criteria, the nucleoprotein and the matrix protein were identified as the 52K and 29K polypeptides, respectively. Immunofluorescence studies using monoclonal antibodies (MAbs) located the glycoprotein on the external cell membrane and the nucleoprotein in the nucleus of infected cells indicating that virus replication involved a nuclear phase. In addition, the virus displayed haemagglutination and haemolytic activities with an optimum at pH 6. These activities are functions of the viral glycoprotein since they were inhibited by antiglycoprotein MAbs. The data reported here support the notion that THO virus is a member of the Orthomyxoviridae family but that it should be classified in a group distinct from the other influenza viruses.

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1992-11-01
2022-01-22
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References

  1. Akov S. 1982; Blood digestion in ticks. In Physiology of Ticks pp. 197–211 Edited by Obenchain F. D., Galun R. Oxford: Pergamon Press;
    [Google Scholar]
  2. Albanese M., Bruno-Smiraglia C., Dicuonzo G., Lavagnino A., Srihongse S. 1972; Isolation of Thogoto virus from Rhipi-cephalus bursa ticks in Western Sicily. Acta virologica 16:267
    [Google Scholar]
  3. Aymard-Henry M., Coleman M. T., Dowdle W. R., Laver W. G., Schild G. C., Webster R. G. 1973; Influenza virus neuraminidase and neuraminidase-inhibition test procedures. Bulletin of the World Health Organization 48:199–202
    [Google Scholar]
  4. Berton M. T., Webster R. G. 1985; The antigenic structure of the influenza B virus hemagglutinin: operational and topological mapping with monoclonal antibodies. Virology 143:583–594
    [Google Scholar]
  5. Choppin P. W., Compans R. W. 1975; Reproduction of paramyxoviruses. In Comprehensive Virology vol. 4 pp. 95–178 Fraenkel-Conrat H., Wagner R. R. New York & London: Plenum Press;
    [Google Scholar]
  6. Clerx J. P. M., Fuller F., Bishop D. H. L. 1983; Tick-borne viruses structurally similar to orthomyxoviruses. Virology 127:205–219
    [Google Scholar]
  7. Davies C. R., Jones L. D., Nuttall P. A. 1986; Experimental studies on the transmission of Thogoto virus, a candidate Orthomyxovirus, in Rhipicephalus appendiculatus ticks. American Journal of Tropical Medicine and Hygiene 35:1256–1262
    [Google Scholar]
  8. Davies C. R., Jones L. D., Green B. M., Nuttall P. A. 1987; In vivo reassortment of Thogoto virus (a tick-borne influenza-like virus) following oral infection of Rhipicephalus appendiculatus ticks. Journal of General Virology 68:2331–2338
    [Google Scholar]
  9. Davies C. R., Jones L. D., Nuttall P. A. 1989; Viral interference in the tick, Rhipicephalus appendiculatus. I. Interference to oral superinfection by Thogoto virus. Journal of General Virology 70:2461–2468
    [Google Scholar]
  10. Fazekas de St Groth S., Scheidegger D. 1980; Production of monoclonal antibodies: strategy and tactics. Journal of Immunological Methods 35:1–21
    [Google Scholar]
  11. Francki R. I. B., Fauquet C. M., Knudson D. L., Brown F. 1991; Classification and nomenclature of viruses. Archives of Virology supplementum 2
    [Google Scholar]
  12. Freedman-Faulstich E. Z., Fuller F. J. 1990; Nucleotide sequence of the tick-borne, orthomyxo-like Dhori/Indian/1313/61 virus envelope gene. Virology 175:10–18
    [Google Scholar]
  13. Fuller F. J., Freedman-Faulstich E. Z., Barnes J. A. 1987; Complete nucleotide sequence of the tick-borne, orthomyxo-like Dhori/Indian/1313/61 virus nucleoprotein gene. Virology 160:81–87
    [Google Scholar]
  14. Fuller F. J., Clay W. C., Freedman E. Z., McEntee M., Barnes J. A. 1989; Nucleotide sequence of the major structural protein genes of the tick-borne, orthomyxo-like Dhori/Indian/ 1313/61 virus. In Genetics and Pathogenicity of Negative Strand Viruses pp. 279–286 Edited by Kolakofsky D., Mahy B. W. J. Amsterdam: Elsevier;
    [Google Scholar]
  15. Gerhard W., Webster R. G. 1978; Antigenic drift in influenza viruses. I. Selection and characterization of antigenic variants of A/PR/8/34 (H0N1) influenza virus with monoclonal antibodies. Journal of Experimental Medicine 148:383–392
    [Google Scholar]
  16. Gottschalk A. 1957; The specific enzyme of influenza virus and Vibrio cholerae . Biochimica et biophysica acta 23:645–646
    [Google Scholar]
  17. Haig D. A., Woodall J. P., Danskin D. 1965; Thogoto virus: a hitherto undescribed agent isolated from ticks in Kenya. Journal of General Microbiology 38:389–394
    [Google Scholar]
  18. Herrler G., Rott R., Klenk H.-D., Müller P., Shukla A. K., Schauer R. 1985; The receptor-destroying enzyme of influenza C virus is neuraminate-O-acetylesterase. EMBO Journal 4:1503–1506
    [Google Scholar]
  19. Herz C., Stavnezer E., Krug R. M. 1981; Influenza virus, an RNA virus, synthesises its messenger RNA in the nucleus of infected cells. Cell 26:391–400
    [Google Scholar]
  20. Holmes K. V., Choppin P. W. 1966; On the role of the response of the cell membrane in determining virus virulence. Journal of Experimental Medicine 124:501–520
    [Google Scholar]
  21. Huang R. T. C., Rott R., Klenk H.-D. 1981; Influenza viruses cause hemolysis and fusion of cells. Virology 110:243–247
    [Google Scholar]
  22. Jones L. D., Nuttall P. A. 1989; The effect of virus-immune hosts on Thogoto virus infection of the tick Rhipicephalus appendiculatus . Virus Research 14:129–140
    [Google Scholar]
  23. Jones L. D., Davies C. R., Green B. M., Nuttall P. A. 1987a; Reassortment of Thogoto virus (a tick-borne influenza-like virus) in a vertebrate host. Journal of General Virology 68:1299–1306
    [Google Scholar]
  24. Jones L. D., Davies C. R., Steele G. M., Nuttall P. A. 1987b; A novel mode of arbovirus transmission involving a non-viremic host. Science 237:775–777
    [Google Scholar]
  25. Jones L. D., Hodgson E., Nuttall P. A. 1989a; Enhancement of virus transmission by tick salivary glands. Journal of General Virology 70:1895–1898
    [Google Scholar]
  26. Jones L. D., Davies C. R., Booth T. F., Nuttall P. A. 1989b; Viral interference in the tick Rhipicephalus appendiculatus. II. Absence of interference with Thogoto virus when the tick gut is by-passed by parenteral inoculation. Journal of General Virology 70:2469–2473
    [Google Scholar]
  27. Köhler G., Milstein C. 1975; Continuous cultures of fused cells secreting antibody of predefined specificity. Nature, London 256:495–497
    [Google Scholar]
  28. Lin D. A., Roychoudhury S., Palese P., Clay W. C., Fuller F. J. 1991; Evolutionary relatedness of the predicted gene product of RNA segment 2 of the tick-borne Dhori virus and the PB1 polymerase gene of influenza viruses. Virology 182:1–7
    [Google Scholar]
  29. Morse M. A., Marriott A. C., Nuttall P. A. 1992; The glycoprotein of Thogoto virus (a tick-borne orthomyxo-like virus) is related to the baculovirus glycoprotein gp64. Virology 186:640–646
    [Google Scholar]
  30. Nuttall P. A., Jones L. D., Davies C. R. 1991; The role of arthropod vectors in arbovirus evolution. Advances in Disease Vector Research 8:15–45
    [Google Scholar]
  31. Srihongse S., Albanese M., Casals J. 1974; Characterization of Thogoto virus isolates from ticks (Rhipicephalus bursa) in Western Sicily, Italy. American Journal of Tropical Medicine and Hygiene 23:1161–1164
    [Google Scholar]
  32. Staunton D., Nuttall P. A., Bishop D. H. L. 1989; Sequence analyses of Thogoto viral RNA segment 3: evidence for a distant relationship between an arbovirus and members of the Orthomyxo-viridae. Journal of General Virology 70:2811–2817
    [Google Scholar]
  33. Webster R. G., Laver W. G. 1980; Determination of the number of nonoverlapping antigenic areas on Hong Kong (H3N2) influenza virus hemagglutinin with monoclonal antibodies and the selection of variants with potential epidemiological significance. Virology 104:139–148
    [Google Scholar]
  34. Zhirnov O. P. 1990; Solubilization of matrix protein M1/M from virions occurs at different pH for orthomyxo- and paramyxoviruses. Virology 176:274–279
    [Google Scholar]
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