1887

Abstract

The nucleotide sequence of a 1.1 kbp fragment of the multiple nucleocapsid nuclear polyhedrosis virus (MNPV) of (Se) containing the polyhedrin gene was determined. An open reading frame (ORF) of 738 nucleotides (nt) was detected. This ORF encoded a protein of 246 amino acids with a predicted of 29K. The nucleotide and amino acid sequences were compared with the sequences of eight other NPV polyhedrins. The SeMNPV polyhedrin protein was most closely related to MNPV polyhedrin with differences in only five amino acids, and most distantly related to the MNPV polyhedrin. The size of the mRNA was approximately 1000 nt, as determined by Northern blot analysis. Using primer extension assays and S1 nuclease mapping the transcriptional start and stop sites of the polyhedrin mRNA were located. The 5′ regulatory sequence appeared to be 44 nt in length with the mRNA start site predominantly at the first A of the TAAG consensus start sequence. Two degenerate poly(A) signals were found immediately downstream of the translational stop signal. The transcriptional stop was located approximately 230 nt downstream from the translational stop signal, in an AT-rich sequence that appears to be common to all baculovirus poly-hedrin genes. The SeMNPV polyhedrin mRNA does not appear to be polyadenylated.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-11-2813
1992-11-01
2024-10-06
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/11/JV0730112813.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-11-2813&mimeType=html&fmt=ahah

References

  1. Birnstiel M. L., Busslinger M., Strub K. 1985; Transcription termination and 3′ processing: the end is in site !. Cell 41:349–359
    [Google Scholar]
  2. Blissard G. W., Rohrmann G. F. 1989; Location, sequence, transcriptional mapping, and temporal expression of gp64 envelope glycoprotein gene of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus. Virology 170:537–555
    [Google Scholar]
  3. Blissard G. W., Rohrmann G. F. 1990; Baculovirus diversity and molecular biology. Annual Review of Entomology 35:127–155
    [Google Scholar]
  4. Caballero P., Zuidema D., Santiago-Alvarez C., Vlak J. M. 1992; Biochemical and biological characterization of four isolates of Spodoptera exigua nuclear polyhedrosis virus. Biocontrol Science and Technology 2:145–157
    [Google Scholar]
  5. Cameron I. R., Possee R. D. 1989; Conservation of polyhedrin gene promoter function between Autographa californica and Mamestra brassicae nuclear polyhedrosis virus. Virus Research 12:183–200
    [Google Scholar]
  6. Chang M. T., Lanner-Herrera C., Fires M. 1989; Nucleotide sequence of Lymantria dispar nuclear polyhedrosis virus polyhedrin gene. Journal of Invertebrate Pathology 53:241–246
    [Google Scholar]
  7. Francki R. I. B., Fauquet C. M., Knudson D., Brown F. (editors) 1991; Classification and nomenclature of viruses. Fifth Report of the International Committee on Taxonomy of Viruses pp. 450
    [Google Scholar]
  8. Gelernter W. D., Federici B. A. 1986a; Isolation, identification, and determination of virulence of a nuclear polyhedrosis virus from the beet army worm, Spodoptera exigua (Lepidoptera: Noctuidae). Environmental Entomology 15:240–245
    [Google Scholar]
  9. Gelernter W. D., Federici B. A. 1986b; Continuous cell line from Spodoptera exigua (Lepidoptera: Noctuidae) that supports replication of nuclear polyhedrosis viruses from Spodoptera exigua and Autographa californica . Journal of Invertebrate Pathology 48:199–207
    [Google Scholar]
  10. Gonzales M. A., Smith G. E., Summers M. D. 1989; Insertion of the SfMNPV polyhedrin gene into an AcMNPV polyhedrin deletion mutant during viral infection. Virology 170:160–175
    [Google Scholar]
  11. Hink F. 1970; Established insect cell line from the cabbage looper, Trichoplusia ni . Nature, London 226:466–467
    [Google Scholar]
  12. Hooft van Iddekinge B. J. L., Smith G. E., Summers M. D. 1983; Nucleotide sequence of the polyhedrin gene of Autographa californica nuclear polyhedrosis virus. Virology 131:561–565
    [Google Scholar]
  13. Howard S. C., Ayres M. D., Possee R. D. 1986; Mapping of the 5′ and 3′ ends of Autographa californica nuclear polyhedrosis virus polyhedrin mRNA. Virus Research 5:109–119
    [Google Scholar]
  14. Iatrou K., Ito K., Witkiewicz H. 1985; Polyhedrin gene of Bombyx mori nuclear polyhedrosis virus. Journal of Virology 54:436–445
    [Google Scholar]
  15. Leisy D., Nesson M., Pearson M., Rohrmann G., Beaudreau G. 1986a; Location and nucleotide sequence of the Orgyia pseudotsugata single nucleocapsid nuclear polyhedrosis virus polyhedrin gene. Journal of General Virology 67:1073–1079
    [Google Scholar]
  16. Leisy D., Rohrmann G., Beaudreau G. 1986b; The nucleotide sequence of the polyhedrin gene region from the multicapsid baculovirus of Orgyia pseudotsugata . Virology 153:280–288
    [Google Scholar]
  17. Leisy D. J., Rohrmann G. F., Nesson M., Beaudreau G. S. 1986c; Nucleotide sequencing and transcriptional mapping of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus pl0 gene. Virology 153:157–167
    [Google Scholar]
  18. Luckow V. L., Summers M. D. 1988; Trends in the development of baculovirus expression vectors. Bio/Technology 6:47–55
    [Google Scholar]
  19. McCutchen B. F., Choudary P. V., Crenshaw R., Maddox D., Kamita S. G., Volrath S., Fowler E., Hammock B. D., Maeda S. 1991; Development of a recombinant baculovirus expressing an insect-selective neurotoxin - potential for pest control. BiojTechnology 9:848–852
    [Google Scholar]
  20. Maeda S. 1989; Expression of foreign genes in insects using baculovirus vectors. Annual Review of Entomology 34:351–372
    [Google Scholar]
  21. Miller L. K. 1988; Baculoviruses as gene expression vectors. Annual Review of Microbiology 42:177–199
    [Google Scholar]
  22. Oakey R., Cameron I. R., Davis B., Davis E., Possee R. D. 1989; Analysis of transcription initiation in the Panolis flammea nuclear polyhedrosis virus polyhedrin gene. Journal of General Virology 70:769–775
    [Google Scholar]
  23. Ohlendorf D. H., Matthews B. W. 1983; Structural studies of protein-nucleic acid interactions. Annual Review of Biophysics and Bioengineering 12:259–284
    [Google Scholar]
  24. Ooi B. G., Rankin C., Miller L. K. 1989; Downstream sequences augment transcription from the essential initiation site of a baculovirus polyhedrin gene. Journal of Molecular Biology 210:721–736
    [Google Scholar]
  25. Possee R. D., Howard S. C. 1987; Analysis of the polyhedrin gene promoter of the Autographa californica nuclear polyhedrosis virus. Nucleic Acids Research 15:10233–10248
    [Google Scholar]
  26. Possee R. D., Sun T.-P., Howard S. C., Ayres M. D., Hill-Perkins M., Gearing K. L. 1992; Nucleotide sequence of the Autographa californica nuclear polyhedrosis 9.4 kbp Eco RI-I and -R (polyhedrin gene) region. Virology 185:229–241
    [Google Scholar]
  27. Rohrmann G. F. 1986; Polyhedrin structure. Journal of General Virology 67:1499–1513
    [Google Scholar]
  28. Rohrmann G. F. 1992; Baculovirus structural proteins. Journal of General Virology 73:749–761
    [Google Scholar]
  29. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn.. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  30. Sanger F., Nicklen S., Coulson A. R. 1977; Nucleotide sequencing with chain terminating inhibitors. Proceedings of the National Academy of Sciences, U. S. A. 74:5463–5467
    [Google Scholar]
  31. Smith G. E., Vlak J. M., Summers M. D. 1983; Physical analysis of Autographa californica nuclear polyhedrosis virus transcripts for polyhedrin and 10000 molecular weight protein. Journal of Virology 45:215–225
    [Google Scholar]
  32. Smith I. R. L., van Beek N. A. M., Podgwaite J. D., Wood H. A. 1988; Physical map and polyhedrin gene sequence of Lymantria dispar nuclear polyhedrosis virus. Gene 71:97–105
    [Google Scholar]
  33. Smits P. H., van de Vrie M., Vlak J. M. 1988; Nuclear polyhedrosis virus for control of Spodoptera exigua on glasshouse crops. Entomologia Experimentalis et Applicata 43:73–80
    [Google Scholar]
  34. Stewart L. M. D., Hirst M., Lopez Ferber M., Merryweather A. T., Caylay P. J., Possee R. D. 1991; Construction of an improved baculovirus insecticide containing an insect-specific toxin gene. Nature, London 352:85–88
    [Google Scholar]
  35. Summers M. D., Smith G. E. 1987; A manual of methods for baculovirus vectors and insect cell culture procedures. Texas Agricultural Experiment Station Bulletin no. 1555
    [Google Scholar]
  36. Tomalski M. D., Miller L. K. 1991; Insect paralysis by baculovirus-mediated expression of a mite neurotoxin gene. Nature, London 352:82–85
    [Google Scholar]
  37. Vlak J. M. 1979; The proteins of nonoccluded nuclear polyhedrosis virus produced in an established cell line of Spodoptera frugiperda . Journal of Invertebrate Pathology 34:110–118
    [Google Scholar]
  38. Vlak J. M. 1992; Genetic engineering of baculoviruses for insect control. In Molecular Approaches to Pure and Applied Entomology pp. 90–127 Edited by Whitten M. J., Oakeshott J. G. New York: Springer-Verlag;
    [Google Scholar]
  39. Vlak J. M., Rohrmann G. F. 1985; The nature of polyhedrin. In Viral Insecticides for Biological Control pp. 489–544 Edited by Maramorosch K., Sherman K. E. Orlando: Academic Press;
    [Google Scholar]
  40. Zanotto P. M. de A., Sampaio M. J. A., Johnson D. W., Rocha T. L., Maruniak J. E. 1992; The Anticarsia gemmatalis nuclear polyhedrosis virus polyhedrin gene region: sequence analysis, gene product and structural comparisons. Journal of General Virology 73:1049–1056
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-73-11-2813
Loading
/content/journal/jgv/10.1099/0022-1317-73-11-2813
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error