1887

Abstract

Congenic mouse strains VM/Dk and VM- /Dk differ at the gene, which controls the incubation period of scrapie in mice; VM/Dk mice are and VM- /Dk mice are . Restriction fragment length polymorphism and DNA sequencing analysis demonstrated that the PrP genes also differ in these strains, confirming the close genetic linkage of and PrP. Using the restriction enzyme I, we have shown that at least 100 kb of DNA flanking the PrP gene differs between the two strains, and therefore the congruence of the and PrP genes is still not certain.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-10-2751
1992-10-01
2022-11-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/10/JV0730102751.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-10-2751&mimeType=html&fmt=ahah

References

  1. Bruce M. E., McConnell I., Fraser H., Dickinson A. G. 1991; The disease characteristics of different strains of scrapie in Sinc congenic mouse lines: implications for the nature of the agent and host control of pathogenesis. Journal of General Virology 72:595–603
    [Google Scholar]
  2. Bueler H., Fischer M., Lang Y., Bluethmann H., Lipp H. P., DeArmond S. J., Prusiner S. B., Aguet M., Weissmann C. 1992; Normal development and behaviour of mice lacking the neuronal cell-surface PrP protein. Nature, London 356:577–582
    [Google Scholar]
  3. Carlson G. A., Kingsbury D. T., Goodman P. A., Coleman S., Marshall S. T., DeArmond S., Westaway D., Prusiner S. B. 1986; Linkage of prion protein and scrapie incubation time genes. Cell 46:503–511
    [Google Scholar]
  4. Carlson G. A., Westaway D., Goodman P. A., Peterson M., Marshall S. T., Prusiner S. B. 1988; Genetic control of prion incubation period in mice. In Novel Infectious Agents and the Central Nervous System (CIBA Foundation Symposium 135) pp. 84–99 Edited by Bock G., Marsh J. London: Wiley-Interscience;
    [Google Scholar]
  5. Carlson G. A., Goodman P. A., Lovett M., Taylor B. A., Marshall S. T., Petersa-Forchia M., Westaway D., Prusiner S. B. 1989; Genetics and polymorphism of the mouse prion gene complex: control of scrapie incubation time. Molecular and Cellular Biology 8:5528–5540
    [Google Scholar]
  6. Cedar H. 1988; DNA methylation and gene activity. Cell 53:3–4
    [Google Scholar]
  7. Dickinson A. G., Meikle V. M. H. 1971; Host-genotype and agent effects in scrapie incubation: change in allelic interaction with different strains of agent. Molecular and General Genetics 112:73–79
    [Google Scholar]
  8. Dickinson A. G., Outram G. W. 1988; Genetic aspects of unconventional virus infections: the basis of the virino hypothesis. In Novel Infectious Agents and the Central Nervous System (Ciba Foundation Symposium 135) pp. 63–83 Edited by Bock G., Marsh J. London: Wiley-Interscience;
    [Google Scholar]
  9. Dickinson A. G., Meikle V. M. H., Fraser H. 1968; Identification of a gene which controls the incubation period of some strains of scrapie agent in mice. Journal of Comparative Pathology 78:293–299
    [Google Scholar]
  10. Falconer D. S. 1989 Introduction to Quantitative Genetics 3rd edn Harlow: Longman;
    [Google Scholar]
  11. Farquhar C. F., Somerville R. A., McBride P., Bruce M. E., Hope J. 1990; PrP detection in peripheral organs of scrapie infected mice. In Abstracts of the VIIIth International Congress of Virology, Berlin, 26–31 August 1990 Abstract P28–013 p 284
    [Google Scholar]
  12. Goldgaber D. 1991).; Anticipating the anti-prion protein?. Nature, London 351:106
    [Google Scholar]
  13. Hewinson R. G., Lowings J. P., Dawson M. D., Woodward M. J. 1991; Anti-prions and other agents. Nature, London 352:291
    [Google Scholar]
  14. Hope J., Morton L. J. D., Farquhar C. F., Multhaup G., Beyreuther K., Kimberlin R. H. 1986; The major polypeptide of scrapie-associated fibrils (SAF) has the same size, charge distribution and N-terminal sequence as predicted for the normal brain protein (PrP). EMBO Journal 5:2591–2597
    [Google Scholar]
  15. Hsiao K. K., Scott M., Foster D., Groth D. F., DeArmond S. J., Prusiner S. B. 1990; Spontaneous neurodegeneration in transgenic mice with mutant prion protein. Science 250:1587–1590
    [Google Scholar]
  16. Hunter N., Hope J., McConnell I., Dickinson A. G. 1987; Linkage of the scrapie-associated fibril protein (PrP) gene and Sinc using congenic mice and restriction fragment length polymorphism analysis. Journal of General Virology 68:2711–2716
    [Google Scholar]
  17. Ikegami Y., Ito M., Isomura H., Momotani E., Sasaki K., Muramatsu Y., Ishiguro N., Shinagawa M. 1991; Preclinical and clinical diagnosis of scrapie by detection of PrP protein in tissues of sheep. Veterinary Record 128:271–275
    [Google Scholar]
  18. Kascsak R. J., Rubenstein R., Merz P. A., Carp R. I., Robakis N. K., Wisniewski H. M., Diringer H. 1986; Immunological comparison of scrapie-associated fibrils isolated from animals infected with four different scrapie strains. Journal of Virology 59:676–683
    [Google Scholar]
  19. Manson J. C., Hope J. 1991; Anti-prions and other agents. Nature, London 352:291
    [Google Scholar]
  20. Merz P. A., Somerville R. G., Wisniewski H. M., Iqbal K. 1981; Abnormal fibrils from scrapie-infected brain. Acta neuropathologica 65:63–74
    [Google Scholar]
  21. Oesch B., Westaway D., Walchli M., McKinley M. P., Kent S. B. H., Aebersold R., Barry R. A., Tempst P., Teplow D. B., Hood L. E., Prusiner S. B., Weissmann C. 1985; A cellular gene encodes scrapie PrP27-30 protein. Cell 40:735–746
    [Google Scholar]
  22. Prusiner S. B., Scott M., Foster D., Pan K.-M., Groth D., Mirenda C., Torchia M., Yang S.-L., Serban D., Carlson G. A., Hoppe P. C., Westaway D., DeArmond S. J. 1990; Transgenetic studies implicate interactions between homologous PrP isoforms in scrapie prion replication. Cell 63:673–686
    [Google Scholar]
  23. Race R. E., Graham K., Ernst D., Caughey B., Chesebro B. 1990; Analysis of linkage between scrapie incubation period and the prion protein gene in mice. Journal of General Virology 71:493–497
    [Google Scholar]
  24. Robakis N. K., Sawh P. R., Wolfe G. C., Rubenstein R., Carp R. I., Innis M. A. 1986; Isolation of a cDNA clone encoding the leader peptide of prion protein and expression of the homologous gene in various tissues. Proceedings of the National Academy of Sciences, U.S.A. 83:6377–6381
    [Google Scholar]
  25. Roderick T. H., Davisson M. T. 1981; Linkage map. In Genetic Variants and Strains of the Laboratory Mouse pp. 279–282 Edited by Green M. C. Stuttgart & New York: Gustav Fischer Verlag;
    [Google Scholar]
  26. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. 1988; Primer directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491
    [Google Scholar]
  27. Thein S. L. 1990; A simplified method of direct sequencing of PCR amplified DNA with Sequenase T7 DNA polymerase. United States Biochemical Editorial Comment 17:26–27
    [Google Scholar]
  28. Westaway D., Goodman P. A., Mirenda C. A., McKinley M. P., Carlson G. A., Prusiner S. B. 1987; Distinct prion proteins in short and long scrapie incubation period mice. Cell 51:651–662
    [Google Scholar]
  29. Westaway D., Mirenda C. A., Foster D., Zebarjadian Y., Scott M., Torchia M., Yang S.-L., Serban H., DeArmond S. J., Ebeling C., Prusiner S. B., Carlson G. A. 1991; Paradoxical shortening of scrapie incubation times by expression of prion protein transgenes derived from long incubation period mice. Neuron 7:59–68
    [Google Scholar]
  30. Wu Y., Brown W. T., Dobkin C., Devine-Gage E., Merz P., Wisniewski H. M. 1987; A Pvu II RFLP detected in the human prion protein (PrP) gene. Nucleic Acids Research 15:3191
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-10-2751
Loading
/content/journal/jgv/10.1099/0022-1317-73-10-2751
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error