Neutralizing Mechanisms of Two Human Monoclonal Antibodies Against Human Cytomegalovirus Glycoprotein 130/55 Free

Abstract

The neutralization of human cytomegalovirus (HCMV) after adsorption to the cell surface at 4 °C was studied using two neutralizing monoclonal antibodies (C-23 and C-41) recognizing glycoprotein 130/55. HCMV adsorbed to cells was neutralized by C-23 (complement-independent), but not by C-41 (complement-dependent). Furthermore, the virus remained sensitive to C-23 for 120 min after shifting up from 4 °C to 37 °C, suggesting that C-23 might block an early stage of virus penetration into cells, and also that transition from virus attachment to virus penetration might be quite slow. The cell-to-cell infection of HCMV was also blocked only by C-23, and not by C-41. On the basis of the results presented here, we suggest that C-41 blocks the attachment of virus to the cell surface whereas C-23 prevents the penetration of virus into the cell.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-10-2705
1992-10-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/10/JV0730102705.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-10-2705&mimeType=html&fmt=ahah

References

  1. Britt W. J. 1984; Neutralizing antibodies detect a disulfide-linked glycoprotein complex within the envelope of human cytomegalovirus. Virology 135:369–378
    [Google Scholar]
  2. Cai W., Gu B., Person S. 1988; Role of glycoprotein B of herpes simplex virus type 1 in viral entry and cell fusion. Journal of Virology 62:2596–2604
    [Google Scholar]
  3. Cranage M. P., Kouzarides T., Satchwell S., Weston K., Tomlinson P., Barrell B., Hart H., Bell S. E., Minson A. C., Smith G. L. 1986; Identification of the human cytomegalovirus glycoprotein B gene and induction of neutralizing antibodies via its expression in recombinant vaccinia virus. EMBO Journal 5:3057–3063
    [Google Scholar]
  4. Fuller A. O., Santos R. E., Spear P. G. 1989; Neutralizing antibodies specific for glycoprotein H of herpes simplex virus permit viral attachment to cells but prevent penetration. Journal of Virology 63:3435–3443
    [Google Scholar]
  5. Gretch D. R., Kari B., Rasmussen L., Gehrz R. C., Stinski M. F. 1988; Identification and characterization of three distinct families of glycoprotein complexes in the envelope of human cytomegalovirus. Journal of Virology 62:875–881
    [Google Scholar]
  6. Kari B., Lussenhop N., Goertz R., Wabuke-Bunoti M., Radeke R., Gehrz R. 1986; Characterization of monoclonal antibodies reactive to several biochemically distinct human cytomegalovirus glycoprotein complexes. Journal of Virology 60:345–352
    [Google Scholar]
  7. Masuho Y., Matsumoto Y.-I., Sugano T., Fujinaga S., Minamishima Y. 1987; Human monoclonal antibodies neutralizing human cytomegalovirus. Journal of General Virology 68:1457–1461
    [Google Scholar]
  8. Pereira L., Hoffman M., Gallo D., Cremer N. 1982; Monoclonal antibodies to human cytomegalovirus three surface membrane proteins with unique immunological and electrophoretic properties specify cross-reactive determinants. Infection and Immunity 36:924–932
    [Google Scholar]
  9. Rasmussen L., Mullenax J., Nelson M., Merigan T. C. 1985; Human cytomegalovirus polypeptides stimulate neutralizing antibody in vivo . Virology 145:186–190
    [Google Scholar]
  10. Tanaka A., Numazaki Y. 1979; Antigenic analysis of human cytomegalovirus isolates from Japanese women and infants. Microbiology and Immunology 23:887–889
    [Google Scholar]
  11. Tomiyama T., Masuho Y. 1990; Antiviral activities of human monoclonal antibody against human cytomegalovirus. In Immunobiology and Prophylaxis of Human Herpesvirus Infections pp. 105–113 Edited by Lopez C. New York: Plenum Press;
    [Google Scholar]
  12. Tomiyama T., Matsumoto Y., Sugano T., Fujinaga S., Masuho Y. 1990; Preparation of human monoclonal antibodies against a cytomegalovirus glycoprotein complex of 130 and 55 kDa. Journal of Immunological Methods 131:249–255
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-10-2705
Loading
/content/journal/jgv/10.1099/0022-1317-73-10-2705
Loading

Data & Media loading...

Most cited Most Cited RSS feed