1887

Abstract

Comparison of sequence data for Broadhaven (BRD) virus, a tick-borne orbivirus, and bluetongue virus (BTV), the type species of the genus, indicated that RNA segments 2 and 7 of BRD virus encode the two structural core proteins, VP2 and VP7, respectively. Segment 2 is 2792 nucleotides in length with a coding capacity for a protein (VP2) of 908 amino acids and a net charge of +8.5 at neutral pH. Segment 7 is 1174 nucleotides in length with a coding capacity for a protein (VP7) of 356 amino acids and a net charge of +11.5 at neutral pH. Comparison of the two sequences with BTV serotype 10 revealed amino acid identity of 35% between the product of segment 2 and BTV VP3, and 21% between the product of segment 7 and BTV VP7. The core proteins therefore show evidence of significant evolutionary divergence compared with that shown between different insect-borne orbiviruses. In particular, the amino terminus of BRD virus VP7 differed markedly from the equivalent region in VP7 of BTV and African horse sickness virus. This region is thought to interact with the outer capsid layer of insect-borne orbiviruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-10-2585
1992-10-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/10/JV0730102585.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-10-2585&mimeType=html&fmt=ahah

References

  1. Eaton B. T., Gould A. R., Hyatt A. D., Coupar B. E. H., Martyn J. C., White J. R. 1991; A bluetongue serogroup-reactive epitope in the amino terminal half of the major core protein VP7 is accessible on the surface of the bluetongue virus particle. Virology 180:687–696
    [Google Scholar]
  2. Gorman B. M. 1983; On the evolution of orbiviruses. Intervirology 20:169–180
    [Google Scholar]
  3. Gould A. R., Pritchard L. E. 1991; Phylogenetic analyses of the complete nucleotide sequence of the capsid protein (VP3) of Australian epizootic haemorrhagic disease of deer virus (serotype 2) and cognate genes from other orbiviruses. Virus Research 21:1–18
    [Google Scholar]
  4. Knudson D. L., Shope R. E. 1985; Overview of the orbiviruses. In Bluetongue and Related Orbiviruses pp. 255–266 Edited by Barber T. L., Jochim M. M. New York: A. R. Liss;
    [Google Scholar]
  5. Kowalik T. F., Li J. K. K. 1991; Bluetongue virus evolution: sequence analysis of the genomic S1 segments and the major core protein VP7. Virology 81:749–755
    [Google Scholar]
  6. Le Blois H., Fayard B., Urakawa T., Roy P. 1991; Synthesis and characterization of chimeric particles between epizootic hemorrhagic disease virus and bluetongue virus: functional domains are conserved on the VP3 protein. Journal of Virology 65:4821–4831
    [Google Scholar]
  7. Mertens P. P. C., Sangar D. V. 1985; Analysis of the terminal sequences of the genome sequences of four orbiviruses. Virology 140:55–67
    [Google Scholar]
  8. Moss S. R., Nuttall P. A. 1986; Isolation and characterisation of temperature sensitive mutants of Broadhaven virus, a Kemerovo group orbivirus (family Reoviridae). Virus Research 4:331–336
    [Google Scholar]
  9. Moss S. R., Ayres C. M., Nuttall P. A. 1987; Assignment of the genome segment coding for neutralizing epitope(s) of orbiviruses in the Great Island subgroup (Kemerovo serogroup). Virology 157:137–144
    [Google Scholar]
  10. Moss S. R., Ayres C. M., Nuttall P. A. 1988; The Great Island subgroup of tick-borne orbiviruses represents a single gene pool. Journal of General Virology 69:2721–2727
    [Google Scholar]
  11. Moss S. R., Fukusho A., Nuttall P. A. 1990; RNA segment 5 of Broadhaven virus, a tick-bome orbivirus, shows sequence homology with segment 5 of bluetongue virus. Virology 179:482–484
    [Google Scholar]
  12. Moss S. R., Jones L. D., Nuttall P. A. 1992; Comparison of the non-structural protein, NS3, of tick-bome and insect-borne orbiviruses. Virology 187:841–844
    [Google Scholar]
  13. Nuttall P. A., Alhaq A., Moss S. R., Carey D., Harrap K. A. 1982; Orbi- and bunyaviruses from a puffin colony in the Outer Hebrides. Archives of Virology 74:259–268
    [Google Scholar]
  14. Purdy M. A., Petre J., Roy P. 1984; Cloning of the bluetongue virus L3 gene. Journal of Virology 51:754–759
    [Google Scholar]
  15. Roy P. 1989; Bluetongue vims genetics and genome structure. Virus Research 13:179–206
    [Google Scholar]
  16. Roy P., Hirasawa T., Fernandez M., Blinov V. M., Sanchez-Vixcain Rodrique J. M. 1991; The complete sequence of the group-specific antigen, VP7, of African horsesickness disease vims serotype 4 reveals a close relationship to bluetongue vims. Journal of General Virology 72:1237–1241
    [Google Scholar]
  17. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A. 74:5463–5467
    [Google Scholar]
  18. Spence R. P., Eley S. M., Nuttall P. A., Pullin J. S. K., Moore N. F. 1985; Replication and polypeptide synthesis of Mill Door/79, an orbivirus isolated from ticks from a seabird colony in Scotland. Journal of Virology 53:705–707
    [Google Scholar]
  19. Spence R. P., Nuttall P. A., Moore N. F. 1986; A comparison of the induced polypeptides and RNAs of the three orbiviruses isolated from ticks (Ixodes uriae) collected in seabird colonies on the Isle of May, Scotland. Acta virologica 39:19–24
    [Google Scholar]
  20. Staden R. 1982; An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences. Nucleic Acids Research 10:2851–2961
    [Google Scholar]
  21. Wilson W. C. 1991; Molecular comparison of VP3 of bluetongue and epizootic haemorrhagic disease vims. Virus Research 21:225–236
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-10-2585
Loading
/content/journal/jgv/10.1099/0022-1317-73-10-2585
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error