1887

Journal of General Virology header logo

Volume 73, Issue 10

Idiotypic Expression of Anti-gp120 Antibodies in Unrelated Human Immunodeficiency Virus-infected Individuals Free

Abstract

Although it is recognized that human immunodeficiency virus (HIV) genes exhibit a high degree of variability, little is known about the molecular heterogeneity of gp120-specific antibodies in infected individuals. As a first step to approach this issue, we investigated the idiotypic relatedness of anti-gp120 antibodies present in the serum of HIV-infected individuals. Idiotypic determinants (idiotopes) are fingerprints of the variable region of the antibody molecule and, as such, they represent unique probes with which to explore the diversity of the immune response. We isolated IgG anti-gp120 antibodies from the serum of a seropositive asymptomatic individual by affinity chromatography. The purified antibodies were shown to bind gp120 and gp160 by ELISA, Western blotting and radio-immunoprecipitation. They also recognized HIV-infected human T cells as detected by immunofluorescence. Anti-idiotypic reagents were generated against this gp120 idiotype, and one of them was used to study anti-gp120 idiotypic diversity in a panel of 65 sera drawn from AIDS and AIDS-related complex patients, and from HIV seropositive asymptomatic individuals. Sixty normal human sera were used as negative controls. We found no evidence for common idiotopes on anti-gp120 antibodies of unrelated individuals. In contrast, we also noticed that the idiotypic profile expressed sequentially at two different intervals in a persistently infected individual showed little variation. Finally, when the diversity of murine anti-gp120 antibodies with a monoclonal anti-idiotype was analysed, no evidence of cross-reactive idiotopes in the murine system was found.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1992
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-10-2561
1992-10-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/10/JV0730102561.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-10-2561&mimeType=html&fmt=ahah

References

  1. Albert J., Abrahamsson B., Nagy K., Aurelius E., Gaines H., Nystrӧm G., Fenyӧ E. V. 1990; Rapid development of isolate-specific neutralizing antibodies after primary HIV-1 infection and consequent emergence of virus variants which resist neutralization by autologous sera. AIDS 4:107–112
     [Google Scholar]
  2. Alizon M., Wain-Hobson S., Montagnier L., Sonigo P. 1986; Genetic variability of the AIDS virus: nucleotide sequence analysis of two isolates from African patients. Cell 46:63–74
     [Google Scholar]
  3. Berman J. E., Mellis S. J., Pollock R., Smith C. L., Suh H., Heinke B., Kowal C., Surti U., Chess L., Cantor C. R., Alt F. W. 1988; Content and organization of the human IgVH locus: definition of three new VH families and linkage to the IgCH locus. EMBO Journal 7:727–738
     [Google Scholar]
  4. Boudet F., Theze J., Zouali M. 1991; UV-treated polystyrene microtiter plates for an ELISA to measure antibodies against synthetic peptides. Journal of Immunological Methods 142:73–82
     [Google Scholar]
  5. Boudet F., Girard M., Theze J., Zouali M. 1992a; Antibodies of HIV-1 positive subjects and experimentally immunized primates and rodents bind to sequence divergent regions of the third variable domain (V3) of gpl20. International Immunology 4:283–294
     [Google Scholar]
  6. Boudet F., David D., Zouali M. 1992b; Idiotypic and genetic expression of the anti-HIV antibody repertoire and its modulation by means of anti-idiotypes. In AIDS Research Reviews Edited by Koff C. W., Wong-Staal G., Kennedy R. C. New York: Marcel Dekker; (in press)
    [Google Scholar]
  7. Corre J. P., Fèvrier M., Chamaret S., Thèze J., Zouali M. 1991; Anti-idiotypic antibodies to human anti-gpl20 antibodies bind recombinant and cellular human CD4. European Journal of Immunology 21:743–751
     [Google Scholar]
  8. Davie J., Sieden M. V., Greenspan N. S., Lutz C. T., Bartholow T. L., Clevinger B. L. 1986; Structural correlates of idiotopes. Annual Review of Immunology 4:147–165
     [Google Scholar]
  9. Devare S. G., Srinivasan A., Bohan C. A., Spira T. J., Curran J. W., Kalyanaraman V. S. 1986; Genomic diversity of the acquired immunodeficiency syndrome retroviruses is reflected in alteration of its translational products. Proceedings of the National Academy of Sciences, U.S.A. 83:5718–5722
     [Google Scholar]
  10. Dreesman G. R., Kennedy R. C. 1985; Anti-idiotypic antibodies: implications of internal image-based vaccines for infectious diseases. Journal of Infectious Diseases 151:761–765
     [Google Scholar]
  11. Fisher A. G., Ensoli B., Looney D., Rose A., Gallo R. C., Saag M. S., Shaw G. M., Hahn B. H., Wong-Staal D. 1988; Biologically diverse molecular variants within a single HIV-1 isolate. Nature, London 334:444–447
     [Google Scholar]
  12. Hahn B., Shaw G., Taylor M., Redfield R., Markham P., Salahuddin F., Wong-Staal F., Gallo R., Parks E., Parks W. 1986; Genetic variation in HTLV/III/LAV over time in patients with AIDS or at risk for AIDS. Science 232:1548
     [Google Scholar]
  13. Javaherian K., Langlois A. J., McDanal C., Ross K. L., Eckler L. I., Jellis C. L., Profy A. T., Rusche J. R., Bolognesi D. P., Putney S. D., Matthews T. L. 1989; Principal neutralizing domain of the human imunodeficiency virus type 1 envelope protein. Proceedings of the National Academy of Sciences, U.S.A. 86:6768–6772
     [Google Scholar]
  14. Kennedy R. C., Dreesman G. R. 1983; Common idiotypic determinant associated with human antibodies to hepatitis B surface antigen. Journal of Immunology 130:385–389
     [Google Scholar]
  15. Kodaira M., Kinashi T., Umemura I., Matsuda F., Noma T., Ono Y., Honjo T. 1986; Organization and evolution of variable region genes of the human immunoglobulin heavy chain. Journal of Molecular Biology 190:529–540
     [Google Scholar]
  16. Krust B., Laurent A. G., Le Guer A., Jeannequin O., Montagnier L., Hovanessian A. G. 1988; Characterization of a monoclonal antibody specific for the HIV-1 precursor glycoprotein. AIDS 2:17–24
     [Google Scholar]
  17. Kunkel H. G., Mannik M., Williams R. G. 1963; Individual antigenic specificity of isolated antibodies. Science 140:1218–1219
     [Google Scholar]
  18. LaRosa G. J., Davide J. P., Weinhold K., Waterbury J. A., Profy A. T., Lewis J. A., Langlois A. J., Dreesman G. R., Boswell R. N., Shadduck P., Holley L. H., Karplus M., Bolognesi D. P., Matthews T. J., Emini E. A., Putney S. D. 1990; Conserved sequence and structural elements in the HIV-1 principal neutralizing determinant. Science 249:932–935
     [Google Scholar]
  19. Morrow W. J. W., Gaston I., Anderson T., Haigwood N., McGrath M. S., Rosen J., Steimer K. S. 1990; Anti-idiotypic antisera raised against monoclonal antibody specific for a p24 gag region epitope detects a common interspecies idiotype associated with anti-HIV responses. Viral Immunology 3:99–109
     [Google Scholar]
  20. Müller S., Wang H. -T., Kaveri S. -V., Chattopadhyay S., Kӧhler H. 1991; Generation and specificity of monoclonal antiidiotypic antibodies against human HIV-specific antibodies. Journal of Immunology 147:933–941
     [Google Scholar]
  21. Oudin J., Michel M. 1963; Une nouvelle forme d’allotypie des globulines y du serum de lapin apparemment liee a la fonction et à la spécificité anticorps. Comptes Rendus de I’Academie des Sciences 257:805–808
     [Google Scholar]
  22. Putney S. D., Matthews T. J., Robey W. G., Lynn D. L., Robert-Guroff M., Mueller W. T., Langlois A. J., Ghrayeb J. 1986; HTLV-III/LAV-neutralizing antibodies to an E. coli-produced fragment of the virus envelope. Science 234:1392–1395
     [Google Scholar]
  23. Rajewsky K., Takemori T. 1983; Genetics, expression, and function of idiotypes. Annual Review of Immunology 1:569–607
     [Google Scholar]
  24. Schwartz O., Henin Y., Marechal V., Montagnier L. 1988; A rapid and simple colorimetric test for the study of anti-HIV agents. AIDS Research and Human Retroviruses 4:441–448
     [Google Scholar]
  25. Srinivasan A., Anand R., York D., Ranganathan P., Feorino P., Schochetman G., Kalyanaraman V. S., Luciw P. A., Sanchez-Pescador R. 1987; Molecular characterization of human immunodeficiency virus from Zaire: nucleotide sequence analysis identifies conserved and variable domains in the envelope gene. Gene 52:71–82
     [Google Scholar]
  26. Tiollais P., Wain-Hobson S. 1984; Molecular genetics of the hepatitis B virus. In Advances in Hepatitis Research pp. 9–20 Edited by Chisari F. New York: Masson Publishing;
     [Google Scholar]
  27. Willey R., Rutledge R., Dias R., Folks T., Theodore T., Buckler C., Martin M. 1986; Identification of conserved and divergent domains within the envelope gene of the acquired immunodeficiency retrovirus. Proceedings of the National Academy of Sciences, U.S.A. 83:5038–5042
     [Google Scholar]
  28. Zouali M., Fine J. M., Eyquem A. 1984a; Anti-DNA autoantibody and idiotypic relationships of human monoclonal proteins. European Journal of Immunology 14:1085–1098
     [Google Scholar]
  29. Zouali M., Fine J. M., Eyquem A. 1984b; A human monoclonal IgGl with anti-idiotypic activity against anti-human thyroglobulin autoantibody. Journal of Immunology 133:190–194
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-10-2561
Loading
Idiotypic Expression of Anti-gp120 Antibodies in Unrelated Human Immunodeficiency Virus-infected Individuals
J. Gen. Virol. 73, 2561 (1992); https://doi.org/10.1099/0022-1317-73-10-2561
/content/journal/jgv/10.1099/0022-1317-73-10-2561
/content/journal/jgv/10.1099/0022-1317-73-10-2561
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error