1887

Abstract

Three bovine herpesvirus type 4 (BHV-4) proteins released into the culture medium of infected cells were identified, with values of 135K, 16K and 14.5K. Among these three proteins, two were precipitated by the monoclonal antibodies characterized in this work. One is a glycoprotein of 135K (gp8) which does not seem to be involved in BHV-4 neutralization. Moreover, this 135K glycoprotein adsorbed onto uninfected susceptible cells. The attachment of gp8 to cells was totally inhibited by the prior adsorption of unlabelled viral proteins. Moreover, anti-gp8 monoclonal antibodies were effective in inhibiting the adsorption of gp8. These results indicate that gp8 could be involved in BHV-4 attachment.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-1-189
1992-01-01
2024-12-09
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/1/JV0730010189.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-1-189&mimeType=html&fmt=ahah

References

  1. Bartha A., Fadol A. M., Lieberman H., Ludwig H., Mohanty S. B., Osorio F. A., Reed D. E., Storz J., Straub O. C., van Der Maaten M. J., Wellemans G. 1987; Problems concerning the taxonomy of the “Movar-type” bovine herpesviruses. Intervirology 28:1–7
    [Google Scholar]
  2. Bennett L. M., Timmins J. G., Thomsen D. R., Post L. E. 1986; The processing of pseudorabies virus glycoprotein gX in infected cells and in uninfected cell line. Virology 155:705–715
    [Google Scholar]
  3. Ben-Porat T., Kaplan A. S. 1970; Synthesis of proteins in cells infected with herpesvirus. V. Viral glycoproteins. Virology 41:265–273
    [Google Scholar]
  4. Chamberlain J. P. 1979; Fluorographic detection of radioactivity in polyacrylamide gels with water-soluble fluor, sodium salicylate. Analytical Biochemistry 98:132–135
    [Google Scholar]
  5. Dubuisson J., Thiry E., Thalasso F., Bublot M., Pastoret P.-P. 1988; Biological and biochemical comparison of bovid herpesvirus-4 strains. Veterinary Microbiology 16:339–349
    [Google Scholar]
  6. Dubuisson J., Thiry E., Bublot M., Sneyers M., Boulanger D., Guillaume J., Pastoret P. P. 1989a; Production and characterization of monoclonal antibodies to bovid herpesvirus-4. Veterinary Microbiology 19:305–315
    [Google Scholar]
  7. Dubuisson J., Boulanger D., Bublot M., Thiry E, Pastoret P.-P. 1989b; Proteins specified by bovine herpesvirus type 4: structural proteins of the virion and identification of two major glycoproteins by using monoclonal antibodies. Journal of General Virology 70:1743–1753
    [Google Scholar]
  8. Dubuisson J., Thiry E., Bublot M., Thomas I., Van Bressem M. F., Coignoul F., Pastoret P. P. 1989c; Experimental infection of bulls with a genital isolate of bovine herpesvirus-4 and reactivation of latent virus with dexamethasone. Veterinary Microbiology 21:97–114
    [Google Scholar]
  9. Dubuisson J., Guillaume J., Boulanger D., Thiry E., Bublot M., Pastoret P.-P. 1990; Neutralization of bovine herpesvirus type 4 by pairs of monoclonal antibodies raised against two glycoproteins and identification of antigenic determinants involved in neutralization. Journal of General Virology 71:647–653
    [Google Scholar]
  10. Dubuisson J., Danyi S., Bublot M., Pastoret P. P., Thiry E. 1991a; Comparison of proteins of simian herpesvirus aotus type 2 and bovine herpesvirus type 4. Journal of General Virology 72:1145–1150
    [Google Scholar]
  11. Dubuisson J., Pastoret P.-P., Thiry E. 1991b; Temporal control of bovine herpesvirus type 4 glycoprotein synthesis. Journal of General Virology 72:1429–1434
    [Google Scholar]
  12. Fuller O. A., Spear P. G. 1985; Specificities of monoclonal and polyclonal antibodies that inhibit adsorption of herpes simplex virus to cells and lack of inhibition by potent neutralizing antibodies. Journal of Virology 55:475–482
    [Google Scholar]
  13. Hampl H., Ben-Porat T., Ehrlicher L., Habermehl K.-O., Kaplan A. S. 1984; Characterization of the envelope proteins of pseudorabies virus. Journal of Virology 52:583–590
    [Google Scholar]
  14. Harlow E., Lane D. 1988; Immunoaffinity purification. In Antibodies:A Laboratory Manual pp 511–552 New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  15. Honess R. W. 1984; Herpes simplex and ‘the herpes complex’: diverse observations and a unifying hypothesis. Journal of General Virology 65:2077–2107
    [Google Scholar]
  16. Israel B. A., Marshall R. L., Letchworth G. J. III 1988; Epitopes specified and protective efficacy of the bovine immune response to bovine herpesvirus-1 glycoprotein vaccines. Vaccine 6:349–356
    [Google Scholar]
  17. Johnson D. C., Wittels M., Spear P. G. 1984; Binding to cells of virosomes containing herpes simplex virus type 1 glycoproteins and evidence for fusion. Journal of Virology 52:238–247
    [Google Scholar]
  18. Kaplan A. S., Erickson J. S., Ben-Porat T. 1975; Synthesis of proteins in cells infected with herpesvirus. X. Proteins excreted by cells infected with herpes simplex virus, types 1 and 2. Virology 64:132–143
    [Google Scholar]
  19. Kuhn J. E., Kramer M. D., Willenbrecher W., Wieland U., Lorentzen E. A., Braun R. W. 1990; Identification of herpes simplex virus type 1 glycoproteins interacting with the cell surface. Journal of Virology 64:2491–2497
    [Google Scholar]
  20. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  21. Liang X., Babiuk L. A., van Drunen Littel-van den Hurk S., Fitzpatrick D. R., Zamb T. J. 1991; Bovine herpesvirus 1 attachment to permissive cells is mediated by its major glycoproteins gI, gIII and gIV. Journal of Virology 65:1124–1132
    [Google Scholar]
  22. Low G. L., Ferguson M. A. J., Futerman A. H., Silman I. 1986; Covalently attached phosphatidylinositol as a hydrophobic anchor for membrane proteins. Trends in Biochemical Sciences 11:212–215
    [Google Scholar]
  23. Ludwig H. 1983; Bovine herpesviruses. In The Herpesviruses vol 2 pp 135–214 Edited by Roizman B. New York & London: Plenum Press;
    [Google Scholar]
  24. Norrild B., Vestergaard B. F. 1979; Immunoelectrophoretic identification and purification of herpes simplex virus antigens released from infected cells in tissue culture. Intervirology 11:104–110
    [Google Scholar]
  25. Okazaki K., Honda E., Minetoma T., Kumagai T. 1987; Bovine herpesvirus type 1 gp87 mediates both attachment of virions to susceptible cells and hemagglutination. Archives of Virology 97:297–307
    [Google Scholar]
  26. Okuno T., Yamanishi K., Shiraki K., Takashi M. 1983; Synthesis and processing of glycoproteins of varicella-zoster virus (VZV) as studied with monoclonal antibodies. Virology 129:357–368
    [Google Scholar]
  27. Randall R. E., Honess R. W. 1980; Proteins of herpesvirus saimiri: identification of two virus polypeptides released into the culture medium of productively infected cells. Journal of General Virology 51:445–449
    [Google Scholar]
  28. Randall R. E., Killington R. A., Watson D. H. 1980; Glycoproteins with type common and type specific antigenic sites excreted from cells infected with herpes simplex virus types 1 and 2. Journal of General Virology 48:297–310
    [Google Scholar]
  29. Sabara M., Gilchrist J. E., Hudson G. R., Babiuk L. A. 1985; Preliminary characterization of an epitope involved in neutralization and cell attachment that is located on the major bovine rotavirus glycoprotein. Journal of Virology 53:58–66
    [Google Scholar]
  30. Staczek J. 1990; Animal cytomegaloviruses. Microbiological Reviews 54:245–265
    [Google Scholar]
  31. Storz J., Ehlers B., Todd W. J., Ludwig H. 1984; Bovine cytomegalovirus: identification and differential properties. Journal of General Virology 65:697–706
    [Google Scholar]
  32. Thiry E., Bublot M., Dubuisson J., Pastoret P.-P. 1989; Bovine herpesvirus-4 (BHV-4) infections of cattle. In Herpesvirus Diseases of Cattle, Horses and Pigs pp 96–115 Edited by Wittmann G. Norwell: Kluwer Academic Publishers;
    [Google Scholar]
  33. Thiry E., Dubuisson J., Bublot M., van Bressem M.-F., Pastoret P.-P. 1990; The biology of bovine herpesvirus-4 infection of cattle. Deutsche tierärztliche Wochenschrift 97:72–77
    [Google Scholar]
  34. Thiry E., Pastoret P.-P., Dessy-Doize C., Hanzen C., Calberg-Bacq C. M., Dagenais L., Vindevogel H., Ectors F. 1981; Reactivation d’un herpesvirus en culture de cellules testiculaires prelevees chez un taureau atteint d’orchite et d’azoospermie. Annales de Médecine Vétérinaire 125:207–214
    [Google Scholar]
  35. van Drunen Littel-van den Hurk S., van den Hurk J. V., Gilchrist J. E., Misra V., Babiuk L. A. 1984; Interactions of monoclonal antibodies and bovine herpesvirus type 1 (BHV-1) glycoproteins: characterization of their biochemical and immunological properties. Virology 135:466–479
    [Google Scholar]
  36. van Zaane D., Brinkhof J. M., Westenbrink F., Gielkens A. L. 1982; Molecular-biological characterization of Marek’s disease virus. 1. Identification of virus-specific polypeptides in infected cells. Virology 121:116–132
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-73-1-189
Loading
/content/journal/jgv/10.1099/0022-1317-73-1-189
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error