RT Journal Article SR Electronic(1) A1 Quade, Kristina A1 Saldanha, John A1 Thomas, Howard A1 Monjardino, JohnYR 1992 T1 Integration of hepatitis B virus DNA through a mutational hot spot within the cohesive region in a case of hepatocellular carcinoma JF Journal of General Virology, VO 73 IS 1 SP 179 OP 182 DO https://doi.org/10.1099/0022-1317-73-1-179 PB Microbiology Society, SN 1465-2099, AB Integrated hepatitis B virus (HBV) DNA previously cloned from a hepatocellular carcinoma genomic library derived from a Japanese patient was characterized further. Sequence analysis of restriction fragments bearing the virus-host junctions defined 3125 nucleotides of essentially un-rearranged HBV DNA of the adr subtype with the right junction mapping within the cohesive region at position 1970 and the left within the pre-core at position 1886. The right viral-host junction contains a 7 bp repeat (TGTAGGC) and a possible 2 bp inversion. The integrated HBV DNA includes the complete open reading frames for core, pre-S, S and polymerase and a 3′ truncated X gene, and lacks most of the pre-core. Integration has occurred at a mutational hot spot of the viral genome and appears to be located in a region of semi-repetitive genomic DNA 3′ to the β-globin gene cluster., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-73-1-179