%0 Journal Article %A Quade, Kristina %A Saldanha, John %A Thomas, Howard %A Monjardino, John %T Integration of hepatitis B virus DNA through a mutational hot spot within the cohesive region in a case of hepatocellular carcinoma %D 1992 %J Journal of General Virology, %V 73 %N 1 %P 179-182 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-73-1-179 %I Microbiology Society, %X Integrated hepatitis B virus (HBV) DNA previously cloned from a hepatocellular carcinoma genomic library derived from a Japanese patient was characterized further. Sequence analysis of restriction fragments bearing the virus-host junctions defined 3125 nucleotides of essentially un-rearranged HBV DNA of the adr subtype with the right junction mapping within the cohesive region at position 1970 and the left within the pre-core at position 1886. The right viral-host junction contains a 7 bp repeat (TGTAGGC) and a possible 2 bp inversion. The integrated HBV DNA includes the complete open reading frames for core, pre-S, S and polymerase and a 3′ truncated X gene, and lacks most of the pre-core. Integration has occurred at a mutational hot spot of the viral genome and appears to be located in a region of semi-repetitive genomic DNA 3′ to the β-globin gene cluster. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-73-1-179