@article{mbs:/content/journal/jgv/10.1099/0022-1317-73-1-179, author = "Quade, Kristina and Saldanha, John and Thomas, Howard and Monjardino, John", title = "Integration of hepatitis B virus DNA through a mutational hot spot within the cohesive region in a case of hepatocellular carcinoma", journal= "Journal of General Virology", year = "1992", volume = "73", number = "1", pages = "179-182", doi = "https://doi.org/10.1099/0022-1317-73-1-179", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-73-1-179", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Integrated hepatitis B virus (HBV) DNA previously cloned from a hepatocellular carcinoma genomic library derived from a Japanese patient was characterized further. Sequence analysis of restriction fragments bearing the virus-host junctions defined 3125 nucleotides of essentially un-rearranged HBV DNA of the adr subtype with the right junction mapping within the cohesive region at position 1970 and the left within the pre-core at position 1886. The right viral-host junction contains a 7 bp repeat (TGTAGGC) and a possible 2 bp inversion. The integrated HBV DNA includes the complete open reading frames for core, pre-S, S and polymerase and a 3′ truncated X gene, and lacks most of the pre-core. Integration has occurred at a mutational hot spot of the viral genome and appears to be located in a region of semi-repetitive genomic DNA 3′ to the β-globin gene cluster.", }