1887

Abstract

The competence of non-hepatocytes to support hepatitis B virus (HBV) gene expression and replication was studied by transient transfection of various human cell lines with a head-to-tail dimer of HBV DNA. Independent of their neuroectodermal, mesenchymal or epithelial origin, all non-hepatocyte cell lines tested synthesized and secreted hepatitis B surface antigen (HBsAg) and hepatitis B core/e antigen (HBc/eAg). Further analyses of two of these cell lines (LS 180 and COLO 320) identified the two major HBV transcripts of 3.6 and 2.2/2.4 kb length, respectively. LS 180 cells were permissive for HBV and duck hepatitis B virus (DHBV) DNA replication and secretion of infectious virions. COLO 320 cells also supported HBV DNA replication, but did not appear to export complete viral particles. These findings provide direct evidence that both HBV and DHBV can replicate in non-hepatic tumour cell lines, one of which is shown also to produce infectious virions.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-1-173
1992-01-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/1/JV0730010173.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-1-173&mimeType=html&fmt=ahah

References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. 1990 Current Protocols in Molecular Biology New York: John Wiley & Sons;
    [Google Scholar]
  2. Blum H. E., Stowring L., Figus A., Montgomery C. K., Haase A. T., Vyas G. N. 1983; Detection of hepatitis B virus DNA in hepatocytes, bile duct epithelium, and vascular elements by in situ hybridization. Proceedings of the National Academy of Sciences, U.S.A. 80:6685–6688
    [Google Scholar]
  3. Blum H. E., Galun E., Liang T. J., von Weizsäcker F., Wands J. R. 1991a; Naturally occurring missense mutation in the polymerase gene terminating hepatitis B virus replication. Journal of Virology 65:1836–1842
    [Google Scholar]
  4. Blum H. E., Liang T. J., Galun E., Wands J. R. 1991b; Persistence of hepatitis B viral DNA after serological recovery from hepatitis B virus infection. Hepatology 14:56–62
    [Google Scholar]
  5. Cattaneo R., Will H., Schaller H. 1984; Hepatitis B virus transcription in the infected liver. EMBO Journal 3:2191–2196
    [Google Scholar]
  6. Chen C., Okayama H. 1987; High efficiency transformation of mammalian cells by plasmid DNA. Molecular and Cellular Biology 7:2745–2752
    [Google Scholar]
  7. Davis L. G., Dibner M. D., Battey J. F. 1986 Basic Methods in Molecular Biology New York: Elsevier;
    [Google Scholar]
  8. Fogh J., Fogh J. M., Orfeo T. 1977; One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. Journal of the National Cancer Institute 59:221–226
    [Google Scholar]
  9. Hirsch R., Colgrove R., Ganem D. 1988; Replication of duck hepatitis B virus in differentiated human hepatoma cell lines after transfection with cloned viral DNA. Virology 167:136–142
    [Google Scholar]
  10. Kaplan P. M., Greenman R. L., Gerin J. L., Purcell R. H., Robinson W. S. 1973; DNA polymerase associated with the human hepatitis B virus antigen. Journal of Virology 12:995–1005
    [Google Scholar]
  11. Kaplan P. M., Ford E. C., Purcell R. H., Gerin J. L. 1976; Demonstration of subpopulation of Dane particles. Journal of Virology 17:885–893
    [Google Scholar]
  12. Korba B. E., Cote P. J., Wells F. V., Baldwin B., Popper H., Purcell R. H., Tennant B. C., Gerin J. L. 1989; Lymphoid cells in the spleen of woodchuck hepatitis virus-infected woodchucks are a site of active viral replication. Journal of Virology 63:1360–1370
    [Google Scholar]
  13. Leibovitz A., Stinson J. C., McCombs W. B., McCoy C. E., Mazur K. C., Marby N. D. 1976; Classification of human colorectal adenocarcinoma cell lines. Cancer Research 36:4562–4569
    [Google Scholar]
  14. Mattes F., Tong K., Teubner K., Blum H. E. 1990; Complete nucleotide sequence of a German duck hepatitis B virus. Nucleic Acids Research 18:6140
    [Google Scholar]
  15. Morgenstern J. P., Land H. 1990; A series of mammalian expression vectors and characterization of their expression of a reporter gene in stably and transiently transfected cells. Nucleic Acids Research 18:1068
    [Google Scholar]
  16. Nakabayashi H., Taketa K., Miyano K., Yamane T., Sato J. 1982; Growth of human hepatoma cell lines with differentiated functions in chemically defined medium. Cancer Research 42:3858–3863
    [Google Scholar]
  17. Omata M. 1990; Significance of extrahepatic replication of hepatitis B virus. Hepatology 12:364–366
    [Google Scholar]
  18. Price J., Turner D., Cepko C. 1987; Lineage analysis in the vertebrate nervous system retrovirus-mediated gene transfer. Proceedings of the National Academy of Sciences, U S A 84:156–160
    [Google Scholar]
  19. Quinn L. A., Moore G. E., Morgan R. T., Woods L. K. 1979; Cell lines from human colon adenocarcinoma with unusual cell production, double minutes and homogeneously staining regions. Cancer Research 39:4914–4924
    [Google Scholar]
  20. Romet-Lemonne J.-L., McLane M. F., Elfassi E., Haseltine W. A., Azocar J., Essex M. 1983; Hepatitis B virus infection in cultured human lymphoblastoid cells. Science 221:667–669
    [Google Scholar]
  21. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  22. Seifer M., Heermann K. H., Gerlich W. H. 1990; Replication of hepatitis B virus in transfected nonhepatic cells. Virology 179:300–311
    [Google Scholar]
  23. Sells M. A., Chen M.-L., Acs G. 1987; Production of hepatitis virus particles in HepG2 cells transfected with cloned hepatitis B virus DNA. Proceedings of the National Academy of Sciences, U.S.A. 84:1005–1009
    [Google Scholar]
  24. Su T.-S., Lui W.-Y., Lin L.-H., Han S.-H., P’eng F.-K. 1989; Analysis of hepatitis B virus transcripts in infected human liver. Hepatology 9:180–185
    [Google Scholar]
  25. Tom B. H., Rutzky L. P., Jakstys M. M., Oyasu R., Kaye C. I., Kahan B. D. 1976; Human colon adenocarcinoma cells. I. Establishment and description of a new line. In Vitro 12:180–191
    [Google Scholar]
  26. Tong S., Diot C., Gripon P., Li J., Vitvitski L., Trepo C., Guguen-Guillouzo C. 1991; In vitro replication competence of a cloned hepatitis B virus variant with a nonsense mutation in the distal pre-c region. Virology 181:733–737
    [Google Scholar]
  27. Walter E., Blum H. E., Offensperger W.-B., Zeschnigk C., Offensperger S., Gerok W. 1987; Spot-blot hybridization assay for the detection of hepatitis B virus in serum: factors determining its sensitivity and specificity. Hepatology 7:557–562
    [Google Scholar]
  28. Walter E., Teubner K., Blum H. E., Offensperger W.-B., Offensperger S., Gerok W. 1991; Duck hepatitis B virus infection of non-hepatocytes. Liver 11:53–62
    [Google Scholar]
  29. Yaginuma K., Shirakata Y., Kobayashi M., Koike K. 1987; Hepatitis B virus (HBV) particles are produced in cell culture system by transient expression of transfected HBV DNA. Proceedings of the National Academy of Sciences, U.S.A. 84:2678–2682
    [Google Scholar]
  30. Yoffe B., Burns D. K., Bhatt H. S., Combes B. 1990; Extrahepatic hepatitis B virus sequences in patients with acute hepatitis B infection. Hepatology 12:187–192
    [Google Scholar]
  31. Zelent A. Z., Sells M. A., Shvartsman M., Price P. M., Acs G. 1987; Replicative intermediate of hepatitis B virus in transfected murine fibroblasts. Journal of Virology 61:2921–2923
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-1-173
Loading
/content/journal/jgv/10.1099/0022-1317-73-1-173
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error