RT Journal Article SR Electronic(1) A1 Hatada, Eriko A1 Takizawa, Takenori A1 Fukuda, RyujiYR 1992 T1 Specific binding of influenza A virus NS1 protein to the virus minus-sense RNA in vitro JF Journal of General Virology, VO 73 IS 1 SP 17 OP 25 DO https://doi.org/10.1099/0022-1317-73-1-17 PB Microbiology Society, SN 1465-2099, AB The non-structural protein NS1, encoded by genome segment 8 of influenza A virus, was expressed in Escherichia coli from cloned cDNA and purified. The NS1 protein had a specific RNA-binding activity, binding to influenza A virus minus-sense but not plus-sense RNA synthesized in vitro from cloned DNA using phage RNA polymerase. NS1 bound preferentially to the regions of RNA containing either 5′- or 3′-terminal common sequences of the genomic RNA. Binding was inhibited by virion RNA, but not by single-stranded minus-sense cDNA and oligo DNAs having the common sequences. In addition, binding was also inhibited by 28S rRNA but not 18S rRNA prepared from MDCK cells., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-73-1-17