1887

Abstract

The non-structural protein NS1, encoded by genome segment 8 of influenza A virus, was expressed in from cloned cDNA and purified. The NS1 protein had a specific RNA-binding activity, binding to influenza A virus minus-sense but not plus-sense RNA synthesized from cloned DNA using phage RNA polymerase. NS1 bound preferentially to the regions of RNA containing either 5′- or 3′-terminal common sequences of the genomic RNA. Binding was inhibited by virion RNA, but not by single-stranded minus-sense cDNA and oligo DNAs having the common sequences. In addition, binding was also inhibited by 28S rRNA but not 18S rRNA prepared from MDCK cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-1-17
1992-01-01
2019-11-20
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-1-17
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error