1887

Abstract

We introduced replicase genes of brome mosaic virus (BMV) to cv. Petit Habana (SR1) using two different types of transformation vectors containing cDNAs of BMV RNA 1 and RNA 2. One type (V type) contains cDNA from which complete viral RNAs are transcribed. These RNAs can function as templates for viral replicase. The other type (M type) contains cDNA from which viral RNAs without their 3′ non-coding regions are transcribed; these RNAs can only function as mRNA. Viral replicase expressed from the integrated cDNAs in both V and M type transgenic plants can complement an infection by BMV RNA 3.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-1-169
1992-01-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/1/JV0730010169.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-1-169&mimeType=html&fmt=ahah

References

  1. Ahlquist P., Bujarski J. J., Kaesberg P., Hall T. C. 1984; Localization of the replicase recognition site within brome mosaic virus RNA by hybrid-arrested RNA sysnthesis. Plant Molecular Biology 3:37–44
    [Google Scholar]
  2. Ahlquist P., French R., Bujarski J. 1987; Molecular studies of brome mosaic virus using infectious transcripts from cloned cDNA. Advances in Virus Research 32:215–242
    [Google Scholar]
  3. Ballas N., Zakai N., Loyter A. 1987; Transient expression of the plasmid pCaMVCAT in plant protoplasts following transformation with polyethylene glycol. Experimental Cell Research 170:228–234
    [Google Scholar]
  4. Bujarski J. J., Hardy S. F., Miller W. A., Hall T. C. 1982; Use of dodecyl-d-maltoside in the purification and stabilization of RNA polymerase from brome mosaic virus-infected barley. Virology 119:465–473
    [Google Scholar]
  5. Ditta G., Stanfield S., Corbin D., Helinski D. R. 1980; Broad range DNA cloning system for Gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proceedings of the National Academy of Sciences, U.S.A. 77:7347–7351
    [Google Scholar]
  6. French R., Janda M., Ahlquist P. 1986; Bacterial gene inserted in an engineered RNA virus: efficient expression in monocotyledonous plant cells. Science 231:1294–1297
    [Google Scholar]
  7. Hardy S. F., German T. L., Loesch-Fries L. S., Hall T. C. 1979; Highly active template-specific RNA-dependent RNA polymerase from barley leaves infected with brome mosaic virus. Proceedings of the National Academy of Sciences, U.S.A. 76:4956–4960
    [Google Scholar]
  8. Horsch R. B., Fry J. E., Hoffmann N. L., Eichholtz D., Rogers S. G., Fraley J. T. 1985; A simple and general method for transferring genes into plants. Science 227:1229–1231
    [Google Scholar]
  9. Kiberstis P. A., Loesch-Fries L. S., Hall T. C. 1981; Viral protein synthesis in barley protoplasts inoculated with native and fractionated brome mosaic virus RNA. Virology 112:804–808
    [Google Scholar]
  10. Lane L. C. 1981; Bromovirus. In Handbook of Plant Virus Infections and Comparative Diagnosis pp 333–376 Edited by Kurstak E. Amsterdam; Elsevier/North-Holland:
    [Google Scholar]
  11. Miller W. A., Dreher T. W., Hall T. C. 1985; Synthesis of brome mosaic virus subgenomic RNA in vitro by internal initiation on (–)-sense genomic RNA. Nature, London 313:68–72
    [Google Scholar]
  12. Mori M., Mise K., Kobayashi K., Okuno T., Furusawa I. 1991; Infectivity of plasmids containing brome mosaic virus cDNA linked to the cauliflower mosaic virus 35s RNA promoter. Journal of General Virology 72:243–246
    [Google Scholar]
  13. Okuno T., Furusawa I. 1979; RNA polymerase activity and protein synthesis in brome mosaic virus-infected protoplasts. Virology 99:218–225
    [Google Scholar]
  14. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U.S.A. 76:4350–4354
    [Google Scholar]
  15. Van Dun, C. M. P., van Vloten-Doting L., Bol J. F. 1988; Expression of alfalfa mosaic virus cDNAl and 2 in transgenic tobacco plants. Virology 163:572–578
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-1-169
Loading
/content/journal/jgv/10.1099/0022-1317-73-1-169
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error