1887

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Volume 73, Issue 1

Translational modulation in hepatitis B virus open reading frame expression Free

Abstract

A series of hepatitis B virus open reading frame (ORF) variants, including mutants in which the relative order of the in-frame start codons (AUG1, AUG2 and AUG3) and nearby sequences had been altered, was expressed both (in HepG2 hepatoblastoma cells) and (by T7 promoter-driven transcription followed by translation in a rabbit reticulocyte lysate). The ratio of the synthesis of the large, middle (M) and major (S) proteins or their chimeric counterparts was analysed to study the translational regulation of ORF expression. As expected on the basis of the ribosome scanning model, the AUG sequence context was found to be a prominent factor in determining the different translational behaviour of the two -specific mRNAs of 2.4 kb (predominantly translated from AUG1) and 2.1 kb (which includes AUG2 and/or AUG3 and can be translated from either). Results from both experimental systems suggested that initiation at internal AUGs in the 2.4 kb RNA is possible. In experiments mutants bearing lesions in a region 5′ to AUG2, which has been implicated in AUG2/AUG3 cis repression, showed no increase in the utilization of internal AUGs. In addition, the chimeric envelope polypeptides produced in transfected HepG2 cells in this study were informative with respect to preS-mediated endoplasmic retention: replacement of the preS2 N terminus with that from preS1 generated a chimeric M protein that was glycosylated within the putative preS1 retention sequence ad was not secreted. Thus, the preS1 retention sequence most likely acts inside the lumen of endoplasmic reticulum and its function is insensitive to glycosylation. A similar element might be active at the N terminus of M protein.

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© Journal of General Virology 1992
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1992-01-01
2024-04-25
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Translational modulation in hepatitis B virus preS-S open reading frame expression
J. Gen. Virol. 73, 139 (1992); https://doi.org/10.1099/0022-1317-73-1-139
/content/journal/jgv/10.1099/0022-1317-73-1-139
/content/journal/jgv/10.1099/0022-1317-73-1-139
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