1887

Abstract

In a previous study we have shown that a single infectious particle of Moloney murine leukaemia virus per cell is sufficient to facilitate chemical carcinogenesis in normal rat kidney cells. When these cells are exposed to the carcinogen after a low number of passages post-infection (p.i.), cell transformation becomes apparent only after many subsequent passages. On the other hand, when exposure is done after a high number of passages p.i., cell transformation can be detected in the treated culture or at the next passage. It is thus evident that whereas the carcinogenic effect is rapid, the viral effect becomes apparent only after a long period of latency. Here we provide evidence that this viral effect requires multiple proviruses and that the long latent period reflects the time needed for a sufficient accumulation of proviruses in some of the cells. This accumulation may result from multiple rounds of superinfection by virions released into the culture medium, although we cannot exclude other mechanisms of provirus amplification. Our data also suggest that this amplification enhances virus production.

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1991-09-01
2022-01-20
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References

  1. Ball J. K., McCarter J. A., Sunderland S. M. 1973; Evidence for helper independent murine sarcoma virus. I. Segregation of replication-defective and transformation-defective viruses. Virology 56:268–284
    [Google Scholar]
  2. Colombo M. P., Ferrari G., Parmiani G., Wettstein P. J. 1988; Retroviral heterogeneity in mouse lymphomas. Oncogene 2:1
    [Google Scholar]
  3. Cooper G. M., Neiman P. 1980; Transforming genes of neoplasms induced by avian lymphoid leukosis viruses. Nature, London 290:475–480
    [Google Scholar]
  4. Dickson C., Smith R., Brooks S., Peters G. 1984; Tumorigenesis by mouse mammary tumor virus: proviral activation of a cellular gene in the common integration region int-2. Cell 37:529–536
    [Google Scholar]
  5. Dudley J., Risser R. 1984; Amplification and novel locations of endogenous mouse mammary tumor virus genomes in mouse T-cell lymphomas. Journal of Virology 49:92–101
    [Google Scholar]
  6. Fan H., Jaenisch R. F., MacIssac P. 1978; Low-multiplicity infection of Moloney murine leukemia virus in mouse cells: effect on number of viral DNA copies and virus production in producer cells. Journal of Virology 28:802–809
    [Google Scholar]
  7. Fung Y. K. T., Crittenden L. B., Kung H. J. 1982; Orientation and position of avian leukosis virus DNA relative to the cellular oncogene c-myc in B-lymphoma tumors of highly susceptible 15I5X75 chickens. Journal of Virology 44:742–746
    [Google Scholar]
  8. Fung Y. K. T., Lewis W. G., Crittenden L. B., Kung H. J. 1983; Activation of cellular oncogene c-erb B by LTR insertion: molecular basis for induction of erythroblastosis by avian leukosis virus. Cell 33:357–368
    [Google Scholar]
  9. Guntaka R. V., Mahy B. W., Bishop J. M., Varmus H. E. 1975; Ethidium bromide inhibits appearence of closed circular viral DNA and integration of virus-specific DNA in duck cells infected with avian sarcoma virus. Nature, London 253:507–511
    [Google Scholar]
  10. Hassan Y., Huleihel M., Priel E., Wolfson M., Aboud M. 1985; Effect of mouse interferon on chemical carcinogenesis in normal rat kidney cells infected with Moloney murine leukemia virus. Carcinogenesis 6:1787–1790
    [Google Scholar]
  11. Hassan Y., Huleihel M., Priel E., Rosner K., Aboud M. 1985; Effect of Moloney murine leukemia virus on the carcinogenicity of 3-methyl-cholanthrene in normal rat kidney cells. Archives of Virology 90:63–71
    [Google Scholar]
  12. Hassan Y., Priel E., Huleihel M., Segal S., Aboud M. 1990; Chemical-retroviral carcinogenic cooperation and its molecular basis in NIH/3T3 cells. Carcinogenesis 11:2097–2102
    [Google Scholar]
  13. Hayward W. S., Neel B. G., Astrin S. M. 1981; Activation of a cellular oncogene by promoter insertion in ALV-induced lymphoid leukosis. Nature, London 290:475–480
    [Google Scholar]
  14. Heidmann T., Heidmann O., Nicolas J. F. 1988; An indicator gene to demonstrate intracellular transposition of defective retroviruses. Proceedings of the National Academy of Sciences, U.S.A 85:2219–2223
    [Google Scholar]
  15. Huleihel M., Aboud M. 1983; Inhibition of retrovirus DNA supercoiling in interferon treated cells. Journal of Virology 48:120–126
    [Google Scholar]
  16. Lane M. A., Neary D., Cooper G. M. 1982; Activation of a cellular transforming gene in tumours induced by Abelson murine leukaemia virus. Nature, London 300:659–661
    [Google Scholar]
  17. Lemay G., Jolicoeur P. 1984; Rearrangement of a DNA sequence homologous to a cell-virus junction fragment in several Moloney murine leukemia virus induced rat thymomas. Proceedings of the National Academy of Sciences, U.S.A 81:38–42
    [Google Scholar]
  18. Lenz J., Celander D., Crowther R. L., Patarca R., Perkins D. W., Haseltine W. A. 1984; Determination of the leukemogenicity of a murine retrovirus by sequences within the long terminal repeat. Nature, London 308:467–470
    [Google Scholar]
  19. Mishra K. N., Pant K. J., Thomas F. O., Price P. J. 1976; Chemical-viral co-carcinogenesis: requirement for leukaemia virus expression in accelerated transformation. International Journal of Cancer 18:852–858
    [Google Scholar]
  20. Neil J. C., Hughes D., McFarlane R., Wilkie N. M., Onions D. E., Lees G., Jarrett O. 1984; Transduction and rearrangement of the myc gene by feline leukaemia virus in naturally occurring T-cell leukaemias. Nature, London 308:814–820
    [Google Scholar]
  21. Nusse R., van Ooyen A., Cox D., Fung Y. K. T., Varmus H. E. 1984; Mode of proviral activation of a putative mammary oncogene (int-1) on mouse chromosome 15. Nature, London 307:131–136
    [Google Scholar]
  22. Payne G. S., Bishop J. M., Varmus H. E. 1982; Multiple arrangements of viral DNA and an activated host oncogene (c-myc) in bursal lymphomas. Nature, London 295:209–217
    [Google Scholar]
  23. Schubach W., Groundine M. 1984; Alteration of c-myc chromatin structure by avian leukosis virus integration. Nature, London 307:702–714
    [Google Scholar]
  24. Shen-Ong G. L. C., Cole M. D. 1984; Amplification of a specific set of intracisternal A-particle genes in mouse plasmocytoma. Journal of Virology 49:171–177
    [Google Scholar]
  25. Shen-Ong G. L. C., Potter M., Mushinski J. F., Lavu S., Reddy P. 1984; Activation of the c-myb locus by viral insertional mutagenesis in plasmacytoid lymphosarcomas. Science 226:1077–1080
    [Google Scholar]
  26. Steffen D. 1984; Proviruses are adjacent to c-myc in some murine leukemia virus-induced lymphomas. Proceedings of the National Academy of Sciences, U.S.A. 81:2097–2101
    [Google Scholar]
  27. Tennant R. W., Rascatti R. J. 1980; Mechanisms of carcinogenesis involving endogenous retroviruses. In Carcinogenesis 5. Modifiers of Chemical Carcinogenesis pp 185–204 Edited by Slaga T. J. New York: Raven Press;
    [Google Scholar]
  28. Tsichlis P. N., Strauss P. G., Hu L. F. 1983; A common region for proviral DNA integration in MoMuLV induced rat thymic lymphomas. Nature, London 302:445–449
    [Google Scholar]
  29. Varmus H. E., Shank P. R. 1976; Unintegrated viral DNA is synthesized in cytoplasm of sarcoma virus transformed duck cells by viral DNA polymerase. Journal of Virology 18:567–573
    [Google Scholar]
  30. Yoshimura F. K., Levine K. L. 1983; AKR thymic lymphoma involving mink cell focus-inducing murine leukemia viruses have a common region of provirus integration. Journal of Virology 45:576–584
    [Google Scholar]
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