The herpesvirus simian agent 8 (SA8) gene which corresponds to the herpes simplex virus (HSV) gene encoding glycoprotein B (gB) was localized, cloned and sequenced. Comparison of its deduced amino acid sequence with those of its counterparts in 12 other distinct herpesviruses was used to evaluate their homology and phylogenetic relationship. The results emphasized that SA8 gB is more closely related to those of HSV-1 and -2, and bovine herpesvirus 2 than to the homologous proteins of other herpesviruses. Furthermore, the alignment showed several regions of domains conserved in the closely related sequences, including four conserved in all the herpesvirus gB sequences examined. The conservation of 10 cysteine residues and most of the proline residues, as well as several potential N-glycosylation sites, suggested that the secondary and tertiary structures of these gBs were similar.
AlbrechtJ. C.,
FleckensteinB.1990; Structural organization of the conserved gene block of herpesvirus saimiri coding for DNA polymerase, glycoprotein B, and a major DNA-binding protein. Virology 174:533–542
BorchersK.,
ÖzelM.,
PauliG.,
GelderblomH.,
LudwigH.1990; Conserved epitopes of simian herpesvirus SA8 and bovine herpesvirus type 2. Archives of Virology 111:1–14
BrownF.1986; The classification and nomenclature of viruses: summary of results of meetings of the International Committee on Taxonomy of Viruses in Sendai, September (1984). Intervirology 25:141–143
BzikD. J.,
FoxB. A.,
DeLucaN. A.,
PersonS.1984; Nucleotide sequence specifying the glycoprotein gene, gB of herpes simplex virus type 1. Virology 133:301–314
BzikD. J.,
DebroyC.,
FoxB. A.,
PedersonN. E.,
PersonS.1986; The nucleotide sequence of the gB glycoprotein gene of HSV-2 and comparison with the corresponding gene of HSV-1. Virology 155:322–333
ConrathsF.,
PauliG.,
LudwigH.1988; Monoclonal antibodies directed against a 130K glycoprotein of bovine herpesvirus 2 cross-react with glycoprotein B of herpes simplex virus. Virus Research 10:53–64
CranageM. P.,
KouzaridesT.,
BankierA. T.,
SatchwellS.,
WestonK.,
TomlinsonP.,
BarrellB.,
HartH.,
BellS. E.,
MinsonA. C.,
SmithG. L.1986; Identification of the human cytomegalovirus glycoprotein B gene and induction of neutralizing antibodies via its expression in recombinant vaccinia virus. EMBO Journal 5:3057–3063
EberleR.,
BlackD.,
HilliardJ. K.1989; Relatedness of glycoproteins expressed on the surface of simian herpesvirus virions and infected cells to specific HSV glycoproteins. Archives of Virology 109:233–252
FengD. F.,
DoolittleR. F.1987; Progressive sequence alignment as a prerequisite to correct phylogenetic trees. Journal of Molecular Evolution 25:351–360
GriffinA. M.1991; The nucleotide sequence of the glycoprotein gB gene of infectious laryngotracheitis virus: analysis and evolutionary relationship to the homologous gene from other herpesviruses. Journal of General Virology 72:393–398
HammerschmidtW.,
ConrathsF.,
MankertzJ.,
PauliG.,
LudwigH.,
BuhkH.-J.1988; Conservation of a gene cluster including glycoprotein B in bovine herpesvirus type 2 (BHV-2) and herpes simplex virus type 1 (HSV-1). Virology 165:388–405
JamesonB. A.,
WolfH.1988; The antigenic index: A novel algorithm for predicting antigenic determinants. Computer Applications in the Biosciences 4:181–186
JohnsonD. C.,
WittlesM.,
SpearP. G.1984; Binding to cells of virosomes containing herpes simplex virus type 1 glycoproteins and evidence for fusion. Journal of Virology 52:238–247
KellerP. M.,
DavisonA. J.,
LoweR. S.,
BennetC. D.,
EllisR. W.1986; Identification and structure of the gene encoding gpII, a major glycoprotein of varicella-zoster virus. Virology 152:181–191
KousoulasK. G.,
PellettP. E.,
PereiraL.,
RoizmanB.1984; Mutations affecting conformation or sequence of neutralizing epitopes identified by reactivity of viable plaques segregate from syn and ts domains of HSV-1 (F) gB gene. Virology 153:379–394
LawmanM. J. P.,
CourtneyR. J.,
EberleR.,
SchafferP. A.,
O’HaraM. K.,
RouseB. T.1980; Cell-mediated immunity to herpes simplex virus: specificity of cytotoxic T cells. Infection and Immunity 30:451–461
LudwigH.,
PauliG.,
GelderblomH.,
DaraiG.,
KochH.-G.,
FlügelR. M.,
NorrildB.,
DanielM. D.1983; B virus (herpesvirus simiae). In The Herpesviruses vol 2 pp 385–428 Edited by
RoizmanB.
New York & London: Plenum Press;
PellettP. E.,
KousoulasK. G.,
PereiraL.,
RoizmanB.1985a; Anatomy of the herpes simplex virus 1 strain F glycoprotein B gene: primary sequence and predicted protein structure of the wild type and of monoclonal antibody-resistant mutants. Journal of Virology 53:242–253
PellettP. E.,
BigginM. D.,
BarrellB.,
RoizmanB.1985b; Epstein–Barr virus genome may encode a protein showing significant amino acid and predicted secondary structure homology with glycoprotein B of herpes simplex virus 1. Journal of Virology 56:807–813
ProudfootN. J.,
BrownleeG. G.1974; Sequence at the 3′ end of globin mRNA shows homology with immunglobulin light chain mRNA. Nature, London 252:359–362
RiggioM. P.,
CullinaneA. A.,
OnionsD. E.1989; Identification and nucleotide sequence of the glycoprotein gB gene of equine herpes virus 4. Journal of Virology 63:1123–1133
RobbinsA. K.,
DorneyD. J.,
WathenM. W.,
WhealyM. E.,
GoldC.,
WatsonR. J.,
HollandL. E.,
WeedS. D.,
LevineM.,
GloriosoJ. C.,
EnquistL. W.1987; The pseudorabies virus gII gene is closely related to the gB glycoprotein gene of herpes simplex virus. Journal of Virology 61:2691–2701
RossL. J. N.,
SandersonM.,
ScottS. D.,
BinnsM. M.,
DoelT.,
MilneB.1989; Nucleotide sequence and characterization of the Marek’s disease virus homologue of glycoprotein B of herpes simplex virus. Journal of General Virology 70:1789–1804
SangerF.,
NicklenS.,
CoulsonA. R.1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
SarmientoM.,
HaffeyM.,
SpearP. G.1979; Membrane proteins specified by herpes simplex viruses. III. Role of glycoprotein VP7 (B2) in virion infectivity. Journal of Virology 29:1149–1158
StevensD. A.,
PincusT.,
BurroughsM. A. K.,
HamparB.1968; Serologic relationship of a simian herpes virus (SA8) and herpes simplex virus: heterogeneity in the degree of reciprocal cross-reactivity shown by rabbit 7S and 19S antibodies. Journal of Immunology 101:979–983
StuveL. L.,
Brown-ShimmerS.,
PachlC.,
NajarianR.,
DinaD.,
BurkeR. L.1987; Structure and expression of the herpes simplex virus type 2 glycoprotein gB gene. Journal of Virology 61:326–335
WhalleyJ. M.,
RobertsonG. R.,
ScottN. A.,
HudsonG. C.,
BellC. W.,
WoodworthL. M.1989; Identification and nucleotide sequence of a gene in equine herpesvirus 1 analogous to the herpes simplex virus gene encoding the major envelope glycoprotein gB. Journal of General Virology 70:383–394
WhitbeckJ. C.,
BelloL. J.,
LawrenceW. C.1988; Comparison of the bovine herpesvirus 1 gI gene and the herpes simplex virus type 1 gB gene. Journal of Virology 62:3319–3327
WitmerL. A.,
RosenthalK. L.,
GrahamF. L.,
FriedmanH. M.,
YeeA.,
JohnsonD. C.1990; Cytotoxic T lymphocytes specific for herpes simplex virus (HSV) studied using adenovirus vectors expressing HSV glycoproteins. Journal of General Virology 71:387–396